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| Rezende,G.L.; Logullo,C.; Meyer,L.; Machado,L.B.; Oliveira-Carvalho,A.L.; Zingali,R.B.; Cifuentes,D.; Galina,A.. |
| In mammals, hexokinase (HK) is strategically located at the outer membrane of mitochondria bound to the porin protein. The mitochondrial HK is a crucial modulator of apoptosis and reactive oxygen species generation. In plants, these properties related to HK are unknown. In order to better understand the physiological role of non-cytosolic hexokinase (NC-HK) in plants, we developed a purification strategy here described. Crude extract of 400 g of maize roots (230 mg protein) contained a specific activity of 0.042 µmol G6P min-1 mg PTN-1. After solubilization with detergent two fractions were obtained by DEAE column chromatography, NC-HK 1 (specific activity = 3.6 µmol G6P min-1 mg PTN-1 and protein recovered = 0.7 mg) and NC-HK 2. A major purification... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Hydrophobic interaction chromatography; Maize; Mitochondrial hexokinase; Non-cytosolic hexokinase; Purification. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000900003 |
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