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| Registros recuperados: 457 | |
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| Alexandra Chittka; Ursula Grazini. |
| During cortical development, neural stem cells (NSCs) switch from proliferative to neuron-generating asymmetric divisions. Here we investigated the role of Schwann cell factor 1 (SC1/PRDM4), a transcriptional repressor highly expressed in the developing nervous system, during NSCs development. We found that SC1 protein levels were down-regulated in newly differentiating neurons, while remaining high in undifferentiated NSCs, suggesting an asymmetric inheritance of SC1. Knockdown of SC1 in the NSCs led to precocious differentiation of neurons and its overexpression led to an increase in Nestin-expressing precursors. We found that SC1, through its amino-terminus, recruits the chromatin modifier PRMT5, a histone arginine methyltransferase that catalyses... |
| Tipo: Poster |
Palavras-chave: Molecular Cell Biology; Neuroscience. |
| Ano: 2010 |
URL: http://precedings.nature.com/documents/4926/version/1 |
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| Heng Ma; Jingying Wang; Erin Lane; Lin Leng; Wenkui Wang; Jun Ren; Richard Bucala; Ji Li. |
| An important role for a macrophage migration inhibitory factor (MIF)-AMP-activated protein kinase (AMPK) signaling pathway in ameliorating myocardial damage following ischemia/reperfusion has been described. An aging-associated reduction in AMPK activity may be associated with a decline in the ability of cardiac cells to activate the MIF-AMPK cascade, thereby resulting in reduced tolerance to ischemic insults. To test this hypothesis, _in vivo_ regional ischemia was induced by occlusion of the left anterior descending (LAD) coronary artery in young (4-6 months) and aged (24-26 months) mice. The ischemic AMPK activation response was impaired in aged hearts compared to young ones (p<0.01). Notably, cardiac MIF expression in aged hearts was lower... |
| Tipo: Manuscript |
Palavras-chave: Molecular Cell Biology; Pharmacology. |
| Ano: 2008 |
URL: http://precedings.nature.com/documents/2466/version/1 |
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| Cheng-Ta Yang; Jhy-Ming Li; Hsu-Huei Weng; Ya-Chin Li; Miao-Fen Chen. |
| Expression of survivin has been reported to be correlated with shorter survival in patients with non-small cell lung cancer (NSCLC), and overexpression of survivin may lead to radioresistance in various human cancers. In this study, we inhibited survivin expression by using an adenoviral vector (AdsiSurvivin)-mediated RNAi to elucidate the combined effect of survivin-targeting gene therapy and radiotherapy on the NSCLC cells. Our data revealed that AdsiSurvivin exerted survivin gene silencing, induced apoptosis, and significantly attenuated the growth potential in NSCLC cells within 72 hours after infection. The combined treatment modalities with AdsiSurvivin infection and radiation were significantly more potent on cell-growth inhibition than monotherapy.... |
| Tipo: Manuscript |
Palavras-chave: Cancer; Molecular Cell Biology. |
| Ano: 2008 |
URL: http://precedings.nature.com/documents/2589/version/1 |
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| Yuriy V. Pershin; Steven La Fontaine; Massimiliano Di Ventra. |
| Recently, behavioural intelligence of the plasmodia of the true slime mold has been demonstrated. It was shown that a large amoeba-like cell Physarum polycephalum subject to a pattern of periodic environmental changes learns and changes its behaviour in anticipation of the next stimulus to come. Currently, it is not known what specific mechanisms are responsible for such behaviour. Here, we show that such behaviour can be mapped into the response of a simple electronic circuit consisting of an LC contour and a memory-resistor (a memristor) to a train of voltage pulses that mimic environment changes. We identify a possible microscopic origin of the memristive behaviour in the Physarum polycephalum, which together with the naturally occurring biological... |
| Tipo: Manuscript |
Palavras-chave: Ecology; Molecular Cell Biology; Bioinformatics. |
| Ano: 2008 |
URL: http://precedings.nature.com/documents/2431/version/1 |
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| Stephen Cooper; Kerby Shedden; Dang Vu-Phan. |
| It is widely accepted that numerous genes are expressed in a cell-cycle dependent manner, with cycle-specific variations in mRNA content or peaks of protein content during the cell cycle. These proposed variations raise the problem of how varying cell cycle gene expression is regulated. This is the "infinite regression" problem or Russian Doll problem where postulating a cell-cycle specific control element merely pushes the explanation of cell-cycle variation back one step to the problem of how that control element itself appears and disappears at particular times during the cell cycle. We present evidence that cyclin mRNA content is invariant during the cell cycle and calculations reveal that mRNA variation does not account for observed... |
| Tipo: Manuscript |
Palavras-chave: Molecular Cell Biology. |
| Ano: 2007 |
URL: http://precedings.nature.com/documents/1218/version/1 |
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| Jian-Ping Lu; Laura Beatty; Jehonathan Pinthus. |
| Identification of novel protein interactions and their mediators is fundamental in understanding cellular processes and is necessary for protein-targeted therapy. Evidently high throughput formatting of these applications in living cells would be beneficial, however no adequate system exists. We present a novel platform technology for the high throughput screening and verification of protein interactions in living cells. The platform’s series of Dual Expression Recombinase Based (DERB) destiny vectors individually encode two sets of recombinase recognizable sequences for inserting the protein open reading frame (ORF) of interest, two sets of promoters and reporter tags in frame with the ORFs for detecting interactions. Introduction into living... |
| Tipo: Manuscript |
Palavras-chave: Biotechnology; Molecular Cell Biology. |
| Ano: 2008 |
URL: http://precedings.nature.com/documents/1550/version/2 |
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| Roberto Serra; Villani Marco; Alessia Barbieri. |
| Cell differentiation is a complex phenomenon whereby a stem cell becomes progressively more specialized and eventually gives rise to a specific cell type. Differentiation can be either stochastic or, when appropriate signals are present, it can be driven to take a specific route. Induced pluripotency has also been recently obtained by overexpressing some genes in a differentiated cell. Here we show that a stochastic dynamical model of genetic networks can satisfactorily describe all these important features of differentiation, and others. The model is based on the emergent properties of generic genetic networks, it does not refer to specific control circuits and it can therefore hold for a wide class of lineages. The model points to a peculiar role of... |
| Tipo: Manuscript |
Palavras-chave: Molecular Cell Biology; Bioinformatics. |
| Ano: 2010 |
URL: http://precedings.nature.com/documents/4741/version/1 |
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| Alfonso E. Islas-Rodríguez; Luz A. García-Madrid. |
| _Trichophyton rubrum_ is the main agent causing dermatophytosis (1). Keratinocytes are
considered to be the first physical barrier of defense against pathogens (2). But not
only a physical barrier. They recognize antigens through Toll like receptors (TLR) (3).
The activation of this TLR, present on the surface of the keratinocytes, induce the
expression of different pro-inflammatory cytokines, co-stimulatory molecules and
antimicrobial peptides such as [beta]-defensins (4).
The main objective of this work is to determine if lipopolysaccharides of G – bacteria
(LPS), lipotheichoic acid from G+ bacteria (LTA), and conidias,... |
| Tipo: Manuscript |
Palavras-chave: Immunology; Microbiology; Molecular Cell Biology. |
| Ano: 2007 |
URL: http://precedings.nature.com/documents/930/version/1 |
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| Lawrence J. Herskowitz; Anthony L. Salvagno; Brian P. Josey; Andy Maloney; Steven J. Koch. |
| Kinesin-1 (conventional kinesin) is a homodimeric motor protein important for axonal transport. It has been well studied through ensemble and single-molecule assays. However, the enzymatic stepping cycle is complex, with many rate constants that are modulated by interaction of the two motor domains. This makes it difficult to predict how changes in a given rate constant may affect observable properties such as processivity, velocity, or stall force. We have written a simulation of kinesin walking using a Stochastic Simulation Algorithm. The model allows for interactions between the heads, and includes states that are not considered part of the normal cycle. This adds to the complexity of the model but also allows for probing rare events, such as those that... |
| Tipo: Poster |
Palavras-chave: Molecular Cell Biology. |
| Ano: 2010 |
URL: http://precedings.nature.com/documents/4468/version/1 |
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| Theodor Ackbarow; Markus J. Buehler. |
| Proteins constitute the elementary building blocks of a vast variety of biological materials such as cellular protein networks, spider silk or bone, where they create extremely robust, multi-functional materials by self-organization of structures over many length- and time scales, from nano to macro. Some of the structural features are commonly found in a many different tissues, that is, they are highly conserved. Examples of such universal building blocks include alpha-helices, beta-sheets or tropocollagen molecules. In contrast, other features are highly specific to tissue types, such as particular filament assemblies, beta-sheet nanocrystals in spider silk or tendon fascicles. These examples illustrate that the coexistence of universality and... |
| Tipo: Manuscript |
Palavras-chave: Biotechnology; Chemistry; Ecology; Molecular Cell Biology. |
| Ano: 2007 |
URL: http://precedings.nature.com/documents/826/version/1 |
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| Saeid Hadi; Hamid Mobasheri. |
| OmpF an outer-membrane porin channel of _E. Coli_ posses beta-barrel structure, whose conductivity and gating phenomena are affected by changes in pH, salt concentration and so on. The distribution of amino acids in OmpF induces a non homologous electric potential in channel lumen. The intrinsic charge distribution caused be amino acid may be affected by lipid bilayer, extra and intra cellular ion concentration, and other physico-chemical condition of environment including, pH, temperature, polyelectrolytes etc. Nucleotides are considered as one of most important metabolite in the cell that have pass through membrane whenever necessary. The experimental study of translocation of such a molecules through channels have provided invaluable information... |
| Tipo: Poster |
Palavras-chave: Molecular Cell Biology. |
| Ano: 2010 |
URL: http://precedings.nature.com/documents/5101/version/1 |
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| Suaib Luqman; Suchita Srivastava. |
| (-)-α-bisabolol is a sesquiterpene alcohol found as a major component of essential oil of chamomile (Matricaria recutita L., Chamomilla recutita L., Matricaria chamomilla L.; Family Asteraceae). Chamomile, one of the most ancient and widely recognized herbs to mankind, has been used traditionally for centuries as an anti-inflammatory, antispasmodic, carminative, mild astringent and healing medicine. It is also known to be very helpful as an external agent for encouraging the rapid healing of ulcers and burns without infection, as well as persistent skin problems such as eczema and psoriasis. Since clinical trials and human studies are limited, we have investigated the effect of (-)-α-bisabolol on markers of oxidative stress in human... |
| Tipo: Poster |
Palavras-chave: Molecular Cell Biology; Pharmacology. |
| Ano: 2012 |
URL: http://precedings.nature.com/documents/6903/version/1 |
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| Jiehua Zhou; Haitang Li; Shirley Li; John Zaia; John Rossi. |
| The successful use of small interfering RNAs (siRNAs) for therapeutic purposes requires safe and efficient delivery to specific cells and tissues. Here we demonstrate cell type-specific delivery of anti-HIV siRNAs via fusion to an anti-gp120 aptamer. The envelope glycoprotein is expressed on the surface of HIV-1 infected cells, allowing binding and interalization of the aptamer-siRNA chimeric molecules. We demonstrate that the anti-gp120 aptamer-siRNA chimera is specifically taken up by cells expressing HIV-1 gp120, and the appended siRNA is processed by Dicer, releasing an anti-tat/rev siRNA which in turn inhibits HIV replication. We show for the first time a dual functioning aptamer-siRNA chimera in which both the aptamer and the siRNA portions have... |
| Tipo: Manuscript |
Palavras-chave: Biotechnology; Molecular Cell Biology. |
| Ano: 2007 |
URL: http://precedings.nature.com/documents/1299/version/1 |
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| Elena N. Mokhova. |
| Some animals are able to survive for a long time under conditions of drastically reduced oxidative metabolism, called metabolic depression. The most investigated type of metabolic depression is hibernation. Research into the basic properties of liver mitochondria energetics during hibernation is essential for fundamental biology and medicine. However, the absence of a suitable hepatocyte culture makes it impossible to study the characteristic features of mitochondrial metabolic states in living cells during hibernation. We proposed that under selected conditions, the unicellular eukaryote Tetrahymena pyriformis resembles hepatocytes under hibernation, as (i) both cell types survive under condition of restricted food supply, hypoxia, and accumulation of... |
| Tipo: Manuscript |
Palavras-chave: Molecular Cell Biology; Evolutionary Biology. |
| Ano: 2011 |
URL: http://precedings.nature.com/documents/4521/version/4 |
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| Anne-Sophie Frojmark; Jitendra Badhai; Joakim Klar; Maria Thuveson; Jens Schuster; Niklas Dahl. |
| Diamond Blackfan anemia (DBA) is a bone marrow failure syndrome associated with heterozygous mutations in the ribosomal protein S19 (RPS19) gene in a subgroup of patients. One of the interacting partners with RPS19 is the oncoprotein PIM-1 kinase. We intercrossed Rps19+/- and Pim-1-/- mice strains to study the effect from the disruption of both genes. The double mutant (Rps19+/-Pim-1-/-) mice display normal growth with increased peripheral white- and red blood cell counts when compared to the w.t. mice (Rps19+/+Pim-1+/+). Molecular analysis of bone marrow cells in Rps19+/-Pim-1-/- mice revealed up-regulated levels of c-Myc and the anti-apoptotic factors Bcl2, BclXL and Mcl-1. This is associated with a reduction of the apoptotic factors Bak and Caspase 3 as... |
| Tipo: Manuscript |
Palavras-chave: Molecular Cell Biology. |
| Ano: 2009 |
URL: http://precedings.nature.com/documents/2882/version/1 |
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| Jayapal J. Manikandan; Renji Reghunathan; Alirio Jose Melendez; Peter Natesan Pushparaj. |
| Receptors for the constant (Fc) region of immunoglobulin G, play an important role in linking the humoral and cellular arms of immune response. Three different classes of receptors have been defined, FcγRI (CD64), FcγRII (CD 32), and FcγRIII (CD16). We have previously shown that in a human monocyte model (IFNγ-primed U937 cells), FcγRI and FcγRII stimulation trigger two very distinct intracellular signalling pathways. Here we show for the first time, the use of oligonucleotide microarrays to investigate the overall gene-expression profile of human monocytes triggered with FcγRI or FcγRII. Our data indicate that several interesting genes are differentially expressed in response... |
| Tipo: Poster |
Palavras-chave: Immunology; Molecular Cell Biology; Bioinformatics. |
| Ano: 2007 |
URL: http://precedings.nature.com/documents/1149/version/1 |
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| Registros recuperados: 457 | |
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