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	Provedor de dados BJM (9) Colegio de Postgraduados (8) Trop. Plant Pathol. (3) BJID (2) Scientia Agricola (2) Genet. Mol. Biol. (2) Horticultura Brasileira (2) Agriscientia (Córdoba) (1) Agrociencia (1) Ciencia e Investigación Agraria (1) Acta Amazonica (1) ArchiMer (1) BABT (1) BJPS (1) Ciência Rural (1) PAB (1) Rev. Bras. Ciênc. Avic. (1) Mais... Autor Hernández Arenas, Marian Guadalupe (2) Jordan Aguilar, Bladimir (2) Morgado González, Aurora (2) Silveira,Wanderley Dias da (2) ALI,Fawad (1) Affara,Nabeel (1) Al-Ghanim,Khalid (1) Almeida,Márcia Rogéria de (1) Almeida-Pereira,Camila S (1) Alvares-Carvalho,Sheila V (1) Alves,Rodrigo P (1) Angelini,Michelle (1) Arisi,Ana Carolina Maisonnave (1) Arrigoni-Blank,Maria de Fatima (1) Arzate-Fernández,Amaury M. (1) Ashraf,Asma (1) Astolfi-Ferreira,CS (1) Audrey,Cilli (1) BALOCH,Faheem Shehzad (1) BARUT,Muzaffer (1) Mais... Palavra-chave Molecular characterization (38) Caracterización molecular (6) RT-PCR (4) Doctorado (3) Fitopatología (3) Maestría (3) Calidad de frutos (2) Caracterización molecular y morfológica (2) Cepas comerciales (2) Commercial strains (2) Conservación postcosecha (2) Cuyoaco (2) EDAR (2) Endophytes (2) Endófitos (2) Estrategias para el Desarrollo Agrícola Regional (2) Fruit quality (2) IBDV (2) Líneas estratégicas (2) Microsatellites (2) Mais... Tipo do documento Info:eu-repo/semantics/article (25) Info:eu-repo/semantics/other (3) Journal article (1) Text (1) Ano 2020 (1) 2019 (1) 2018 (3) 2017 (1) 2016 (1) 2015 (5) 2014 (1) 2012 (3) 2011 (6) 2010 (3) 2009 (3) 2008 (1) 2007 (2) 2006 (2) 2005 (1) 2004 (2) 2001 (2) Mais... País Brazil (26) Mexico (9) Argentina (1) Chile (1) France (1) Idioma Inglês (30) Espanhol (8)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 38 Primeira ... 1 2 ... Última Caracterización y selección de genotipos de cepas comerciales de "setas" (Pleurotus), como acción estratégica para la producción rural en Cuyoaco, Puebla. Colegio de Postgraduados Morgado González, Aurora. Existe una gran diversidad de cepas comerciales de Pleurotus (setas) que no han sido caracterizadas tanto molecular como morfológicamente en los ceparios del país, este material biológico almacenado, posee una amplia variedad de características deseables para la producción rural, así como para el almacenamiento y consumo. En la presente investigación se realizó la caracterización molecular y morfológica de 17 cepas comerciales. Así mismo, las cepas se sembraron en invierno (2009) y primavera (2010) para determinar su precocidad y productividad, así como la estimación de la vida de anaquel, y finalmente se realizó una prueba hedónica en el Colegio de Postgraduados Campus Puebla y otra en la ciudad de Libres, Puebla. En el análisis filogenético se obtuvieron... Palavras-chave: Cepas comerciales; Pleurotus spp; Caracterización molecular y morfológica; Selección de cepas; Cuyoaco; Commercial strains; Molecular characterization; Mushroom production; Puebla; Maestría; Estrategias para el Desarrollo Agrícola Regional; EDAR. Ano: 2011 URL: http://hdl.handle.net/10521/445 Contenidos de taninos y caracterización molecular de variedades de haba (Vicia faba L.) cultivadas por agricultores. Colegio de Postgraduados Jordan Aguilar, Bladimir. En México el haba (Vicia faba L.) es importante para la alimentación de los habitantes del medio rural y urbano, contiene un alto contenido nutritivo, es un alimento balanceado, asimismo en la actualidad los productores demandan variedades con mayor calidad de semilla. El rango en cuanto al contenido de taninos en las semillas de haba cultivadas por los agricultores fue determinado, se estimaron las relaciones entre las variedades de haba mediante la utilización de marcadores moleculares tipo RAPD y microsatélites y se propone una estrategia de aprovechamiento de las variedades por los productores de haba con base a los caracteres estudiados. El desarrollo de este estudio se realizó en dos etapas, la primera consistió en la determinación del contenido de... Palavras-chave: Taninos; Caracterización molecular; Variedades de haba; Líneas estratégicas; Tannin; Molecular characterization; Varieties of faba bean; Strategy lines. Ano: 2011 URL: http://hdl.handle.net/10521/438 Contenidos de taninos y caracterización molecular de variedades de haba (Vicia faba L.) cultivadas por agricultores. Colegio de Postgraduados Jordan Aguilar, Bladimir. En México el haba (Vicia faba L.) es importante para la alimentación de los habitantes del medio rural y urbano, contiene un alto contenido nutritivo, es un alimento balanceado, asimismo en la actualidad los productores demandan variedades con mayor calidad de semilla. El rango en cuanto al contenido de taninos en las semillas de haba cultivadas por los agricultores fue determinado, se estimaron las relaciones entre las variedades de haba mediante la utilización de marcadores moleculares tipo RAPD y microsatélites y se propone una estrategia de aprovechamiento de las variedades por los productores de haba con base a los caracteres estudiados. El desarrollo de este estudio se realizó en dos etapas, la primera consistió en la determinación del contenido de... Palavras-chave: Taninos; Caracterización molecular; Variedades de haba; Líneas estratégicas; Tannin; Molecular characterization; Varieties of faba bean; Strategy lines. Ano: 2011 URL: http://hdl.handle.net/10521/438 Nacobbus aberrans en cultivos hortícolas del Centro del Bajío, México : distribución, abundancia y caracterización molecular. Colegio de Postgraduados Cabrera Hidalgo, Anselmo de Jesús. Nacobbus aberrans es un fitoparásito con una alta capacidad reproductiva y amplia gama de hospedantes, lo que lo convierte en un patógeno importante y de difícil manejo. Presenta una gran variabilidad morfológica y patogénica, y se distribuye ampliamente en las regiones tropicales y templadas del continente Americano, parasitando a más de 84 especies cultivadas y no cultivadas pertenecientes a 18 familias botánicas. Los objetivos de esta investigación fueron analizar su distribución y abundancia en la región central del Bajío mexicano, y su asociación con las características fisicoquímicas del suelo. Adicionalmente, se evaluó su variabilidad genética y el efecto del fluensulfone sobre su movilidad en condiciones in vitro y en invernadero. El nematodo se... Palavras-chave: N. aberrans; Distribución espacial; Fluensulfone; PCR-RFLPs; Caracterización molecular; Spatial distribution; Molecular characterization; Fitopatología; Doctorado. Ano: 2014 URL: http://hdl.handle.net/10521/2241 Caracterización cualitativa de frutos de rambutan (Nephelium lappaceum L.), almacenamiento postcosecha y patógenos asociados. Colegio de Postgraduados Hernández Arenas, Marian Guadalupe. El rambutan (Nephelium lappaceum L.) es un frutal tropical originario de Asia e introducido a México. Es cultivado principalmente en el estado de Chiapas pero su comercialización es limitada por una corta vida en anaquel, oscurecimiento del pericarpio y enfermedades postcosecha. Los objetivos de este trabajo fueron: 1) caracterizar frutos de cinco selecciones de rambután procedentes del estado de Chiapas; 2) estudiar el efecto de una cera y dos atmósferas modificadas a temperatura ambiente y refrigeración sobre la calidad visual y vida en anaquel de los frutos y 3) identificar el agente causal de la mancha negra del rambutan en postcosecha. Los frutos fueron cosechados entre Junio y Agosto del 2007 y 2008 en Tuxtla Chico, Chiapas. Se realizaron estudios de... Palavras-chave: Calidad de frutos; Conservación postcosecha; Patógenos; Endófitos; Caracterización molecular; Fruit quality; Postharvest conservation; Pathogens; Endophytes; Molecular characterization; Doctorado; Fitopatología. Ano: 2010 URL: http://hdl.handle.net/10521/155 Caracterización cualitativa de frutos de rambutan (Nephelium lappaceum L.), almacenamiento postcosecha y patógenos asociados. Colegio de Postgraduados Hernández Arenas, Marian Guadalupe. El rambutan (Nephelium lappaceum L.) es un frutal tropical originario de Asia e introducido a México. Es cultivado principalmente en el estado de Chiapas pero su comercialización es limitada por una corta vida en anaquel, oscurecimiento del pericarpio y enfermedades postcosecha. Los objetivos de este trabajo fueron: 1) caracterizar frutos de cinco selecciones de rambután procedentes del estado de Chiapas; 2) estudiar el efecto de una cera y dos atmósferas modificadas a temperatura ambiente y refrigeración sobre la calidad visual y vida en anaquel de los frutos y 3) identificar el agente causal de la mancha negra del rambutan en postcosecha. Los frutos fueron cosechados entre Junio y Agosto del 2007 y 2008 en Tuxtla Chico, Chiapas. Se realizaron estudios de... Palavras-chave: Calidad de frutos; Conservación postcosecha; Patógenos; Endófitos; Caracterización molecular; Fruit quality; Postharvest conservation; Pathogens; Endophytes; Molecular characterization; Doctorado; Fitopatología. Ano: 2010 URL: http://hdl.handle.net/10521/155 Caracterización organísmica y genética en poblaciones de Morchella spp. en bosques de Abies religiosa del centro de México. Colegio de Postgraduados Hernández Hernández, Ismael. Se realizó la caracterización organísmica y genética en poblaciones de Morchella spp. de bosques puros de Abies religiosa de la región Central de México, con niveles contrastantes de contaminación atmosférica. El trabajo se desarrolló en dos fases: la primera en campo y la segunda en el laboratorio. En la fase de campo se emplearon sitios permanentes de tamaño variable en: (1) área de influencia del Parque Nacional Desierto de los Leones, D.F. (DDL); (2) Cerro Tláloc, Edo. Mex. (T); (3) área de influencia del Parque Nacional Izta-Popo, Puebla (IP) y (4) Reserva de la Biósfera de la Mariposa Monarca, Mich (MM); para el muestro de ascomas y suelo adyacente con raíces. La fase de laboratorio consistió en: (1) Pruebas de identificación ascomas-raíces de... Palavras-chave: Abies religiosa; Caracterización molecular; Incompatibilidad somática; Espacio transcrito interno; Morchella; Número de núcleos; Molecular characterization; Somatic incompatibility; Internal transcribed spacer; Number of nuclei; Genética; Maestría. Ano: 2012 URL: http://hdl.handle.net/10521/1724 Caracterización y selección de genotipos de cepas comerciales de "setas" (Pleurotus), como acción estratégica para la producción rural en Cuyoaco, Puebla. Colegio de Postgraduados Morgado González, Aurora. Existe una gran diversidad de cepas comerciales de Pleurotus (setas) que no han sido caracterizadas tanto molecular como morfológicamente en los ceparios del país, este material biológico almacenado, posee una amplia variedad de características deseables para la producción rural, así como para el almacenamiento y consumo. En la presente investigación se realizó la caracterización molecular y morfológica de 17 cepas comerciales. Así mismo, las cepas se sembraron en invierno (2009) y primavera (2010) para determinar su precocidad y productividad, así como la estimación de la vida de anaquel, y finalmente se realizó una prueba hedónica en el Colegio de Postgraduados Campus Puebla y otra en la ciudad de Libres, Puebla. En el análisis filogenético se obtuvieron... Palavras-chave: Cepas comerciales; Pleurotus spp; Caracterización molecular y morfológica; Selección de cepas; Cuyoaco; Commercial strains; Molecular characterization; Mushroom production; Puebla; Maestría; Estrategias para el Desarrollo Agrícola Regional; EDAR. Ano: 2011 URL: http://hdl.handle.net/10521/445 Cloning, mapping and molecular characterization of porcine progesterone receptor membrane component 2 (PGRMC2) gene Genet. Mol. Biol. Chen,Congying; Sargent,Carole; Quilter,Claire; Yang,Zhuqing; Ren,Jun; Affara,Nabeel; Brenig,Bertram; Huang,Lusheng. Progesterone plays an important role in sow reproduction by stimulating classic genomic pathways via nuclear receptors and non-genomic pathways via membrane receptors such a progesterone receptor membrane component 2 (PGRMC2). In this work, we used radiation hybrid mapping to assign PGRMC2 to pig chromosome 8 and observed that this receptor has two transcripts in pigs. The full-length cDNA of the large transcript is 1858 bp long and contains a 669-bp open reading frame (ORF) encoding a protein of 223 amino acids. The shorter transcript encodes a protein of 170 amino acids. The porcine PGRMC2 gene consists of three exons 446 bp, 156 bp and 1259 bp in length. The promoter sequence is GC-rich and lacks a typical TATA box. Several putative cis-regulatory DNA... Tipo: Info:eu-repo/semantics/article Palavras-chave: Expression profile; Molecular characterization; Physical mapping. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000300015 Microsatellite variability analysis in farmed catfish (Ictalurus punctatus) from Tamaulipas, Mexico Genet. Mol. Biol. Perales-Flores,Laura E.; Sifuentes-Rincón,Ana María; León,Francisco J. García de. Analysis of cultured catfish from six farms in Tamaulipas, Mexico was achieved using a combination of microsatellite PCR analysis and semiautomatic fluoresce-based detection, in order to provide a first assessment of the genetic variability on cultured catfish in Mexico. Five microsatellites showed extensive polymorphism with allele numbers ranging from 10 and 20. Overall observed heterozygosity at each locus ranged between 0.76 and 0.91 and the average polymorphic information content (PIC) for the five loci was 0.811, indicating that these loci can be used for studies of paternity identification, linkage and population genetics. On the basis of the F ST values (F ST = 0.03829; p = 0.00000) it appears that there was a small amount of genetic... Tipo: Info:eu-repo/semantics/other Palavras-chave: Ictaluridae; Molecular characterization; Genetic variability. Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400011 Genetic similarity between coriander genotypes using ISSR markers Horticultura Brasileira Melo,Roberto de A; Resende,Luciane V; Menezes,Dimas; Beck,Ana Paula A; Costa,José Carlos da; Coutinho,Alisson E; Nascimento,Ana Verônica S do. With the development of new cultivars, a precise genetic characterization is essential for improvement programs or for cultivar registration and protection. Molecular markers have been complementing the traditional morphological and agronomic characterization techniques because they are virtually unlimited, cover the whole genome and are not environmentally influenced. Genetic characterization constitutes the basis for studies involving estimates of genetic similarity. Therefore, the objective of the present study was to evaluate the genetic similarity between ten coriander genotypes (nine cultivars and one line) using ISSR markers. The cultivars used were: Americano, Asteca, Palmeira, Português, Santo, Supéria, Tabocas, Tapacurá, Verdão and the... Tipo: Info:eu-repo/semantics/article Palavras-chave: Coriandrum sativum L; Molecular characterization; Microsatellites; Condiments. Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362011000400014 Genetic divergence in basil cultivars and hybrids Horticultura Brasileira Alves,Rodrigo P; Silva,Ana Veruska C; Almeida-Pereira,Camila S; Costa,Tatiana S; Alvares-Carvalho,Sheila V; Arrigoni-Blank,Maria de Fatima; Blank,Arie F. ABSTRACT Basil is an aromatic herb that stands out for its economic importance. It is consumed in natura and used to obtain essential oil. The cultivation of this species in several regions of the world has allowed variations by natural crosses and euploidy, leading to the wide genetic variability found nowadays. Considering the importance of this species, we aimed to analyze the genetic diversity of 27 basil genotypes using ISSR molecular markers. Fourteen primers were employed for DNA amplification, resulting in 86% polymorphism. Based on the Jaccard’s dissimilarity index, the highest index (0.80) was observed between the individuals BAS001 and BAS012, while the lowest index (0.18) was detected between the genotypes BAS014 and BAS015. The genetic... Tipo: Info:eu-repo/semantics/article Palavras-chave: Ocimum basilicum; Diversity; Molecular characterization; Dominant marker. Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362019000200180 Microbial communities of recirculating aquaculture facilities: interaction between heterotrophic and autotrophic bacteria and the system itself ArchiMer Michaud, Luigi. Recirculating Aquaculture Systems (RAS) may be considered an alternative to pond aquaculture technology (by consuming lower amounts of water producing similar yields of crop) or can be integrated in a sort of "production chain" with net pen systems. The interest in RAS is due to their intrinsic advantages like the reduction of land and water requirements, the high degree of environmental control allowing year-round growth at optimum rates and the possibility to produce in close proximity to markets. Moreover, the high level of control improves risk management linked with bacterial and parasitic infections and predator pests. Finally, waste production (both particulate and soluble) can be controlled and minimized contributing to make these systems... Tipo: Text Palavras-chave: Conceptual model; Molecular characterization; Biological filter; Microbial ecology; Recirculating Aquaculture System; Modèle conceptuel; Bioactivateur; Caractérisation moléculaire; Carbone organique particulaire; Filtre biologique; Écologie microbienne; Aquaculture en circuit recirculé. Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/these-2682.pdf Rescue of a non-viable accession and rapd analysis of recovered plants of Arachis retusa PAB Gagliardi,Rachel Fatima; Pacheco,Georgia Pereira; Oliveira,Carlos Alberto; Carneiro,Leonardo Alves; Valls,José Francisco Montenegro; Vieira,Maria Lucia Carneiro; Mansur,Elisabeth. In vitro regeneration of Arachis retusa was examined for the purpose of germplasm renewal and conservation. Random amplified polymorphic DNA (RAPD) fingerprinting was used to evaluate the genetic stability of plants derived from embryo axes and apical segments. Ten arbitrary decamer primers were screened and five of them were selected. Ninety genomic regions were evaluated, with an average of 18 loci per clone. All amplified segments were monomorphic. The results indicate that recovered plants are genetically stable at the assessed genomic regions and that both regeneration processes are suitable for in vitro germplasm preservation of Arachis species. Tipo: Info:eu-repo/semantics/article Palavras-chave: Germplasm conservation; Micropropagation; Molecular characterization; Genetic stability. Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2004000200015 Detection by Rt-Pcr and Molecular Characterization of Tremovirus A Obtained from Clinical Cases of Avian Encephalomyelitis (AE) Outbreaks in Brazil Rev. Bras. Ciênc. Avic. Torre,D De la; Nuñez,LF; Parra,SHS; Astolfi-Ferreira,CS; Ferreira,AJP. ABSTRACT This study determined the presence of Tremovirus A as the possible agent related to Avian Encephalomyelitis in broiler chicks from the states of São Paulo (SP) Paraná (PR), Goiás (GO), Santa Catarina (SC) and Rio Grande do Sul (RS), between the years 2006 and 2015. Samples of the nervous, digestive, respiratory, immune, and renal systems, plus muscular organs from broiler chicks with neurological problems such as ataxia and muscle tremors, and four (4) commercial vaccines as positive control, were tested by reverse-transcriptase (RT-PCR) amplification and DNA sequencing. A highly conserved region (P1) of the viral genome, was used to amplify a segment which encodes a structural protein VP4. Out of 112 samples, 46 were positive (42%) for Tremovirus... Tipo: Info:eu-repo/semantics/article Palavras-chave: Chicken; Tremovirus A; Avian Encephalomyelitis; Detection; Molecular characterization. Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2018000300527 Development of an SSR-based identification key for Tunisian local almonds Scientia Agricola Gouta,Hassouna; Ksia,Elhem; Buhner-Zaharieva,Tatiana; Mliki,Ahmed; Gogorcena,Yolanda. Ten simple sequence repeat (SSR) loci were used to study polymorphism in 54 almond genotypes. All genotypes used in this study originated from almond-growing areas in Tunisia with different climatic conditions ranging from the sub-humid to the arid and are preserved in the national collection at Sidi Bouzid. Using ten SSR, 130 alleles and 250 genotypes were revealed. In order to develop an identification key for each accession, the data were analysed separately for each microsatellite marker. The most polymorphic microsatellite (CPDCT042) was used as a first marker. Two microsatellite loci (CPDCT042 and CPDCT025) were sufficient to discriminate among all accessions studied. Neighbour-joining clustering and principal coordinate analysis were performed to... Tipo: Info:eu-repo/semantics/article Palavras-chave: Prunus dulcis Mill; Genetic diversity; Genetic resources; Microsatellites; Molecular characterization. Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162012000200004 Genetic similarity of Brazilian hull-less and malting barley varieties evaluated by RAPD markers Scientia Agricola Kroth,Mariela Aparecida; Ramella,Micheline Sandra; Tagliari,Caroline; Francisco,Alicia de; Arisi,Ana Carolina Maisonnave. Barley (Hordeum vulgare L.) is widely used for brewing and animal feed. Recently, it has become desirable for human consumption due to its high nutritional significance, specially hull-less or naked barley. There are differences in nutritional and malting characteristics among barley varieties. RAPD procedure is able to separate barley varieties at various similarity levels. The aim of this work was the RAPD analysis of six Brazilian hull-less varieties and seven malting varieties. PCR reactions were performed with eleven random primers. A total of 34 RAPD fragments was obtained with five primers. A dendrogram was constructed based on the Jaccard similarity coefficient. Barley varieties displayed an average similarity coefficient of 0.53. Intravarietal... Tipo: Info:eu-repo/semantics/article Palavras-chave: Hordeum vulgare; Polymorphism; Molecular characterization. Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162005000100007 Sequence analysis of the capsid protein gene of an isolate of Apple stem grooving virus, and its survey in Southern Brazil Trop. Plant Pathol. NICKEL,OSMAR; FAJARDO,THOR V.M.; JELKMANN,WILHELM; KUHN,GILMAR B.. Apple stem grooving virus (ASGV) is one of the most important viruses infecting fruit trees. This study aimed at the molecular characterization of ASGV infecting apple (Malus domestica) plants in Santa Catarina (SC). RNA extracted from plants infected with isolate UV01 was used as a template for RT-PCR using specific primers. An amplified DNA fragment of 755 bp was sequenced. The coat protein gene of ASGV isolate UV01 contains 714 nucleotides, coding for a protein of 237 amino acids with a predicted Mr of approximately 27 kDa. The nucleotide and the deduced amino acid sequences of the coat protein gene showed identities of 90.9% and 97.9%, respectively, with a Japanese isolate of ASGV. Very high amino acid homologies (98.7%) were also found with Citrus... Tipo: Info:eu-repo/semantics/other Palavras-chave: Molecular characterization; Capillovirus; RT-PCR. Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582001000300014 Genetic divergence among and within Colletotrichum lindemuthianum races assessed by RAPD Trop. Plant Pathol. Talamini,Viviane; Souza,Elaine A.; Pozza,Edson A.; Silva,Gilvan F.; Ishikawa,Francine H.; Camargo Júnior,Osnil A.. Genetic divergence within and among races of Colletotrichum lindemuthianum was determined using RAPD markers. In addition to the different races of the fungus three isolates of the sexual stage of Colletotrichum lindemuthianum (Glomerella cingulata f.sp. phaseoli) were included in this study. The band patterns generated using 11 primers produced 133 polymorphic bands. The polymorphic bands were used to determine genetic divergence among and within the pathogen races. The isolates analyzed were divided into six groups with 0.75 relative similarity. Group VI, formed by three isolates of the sexual phase of Colletotrichum lindemuthianum, was the most divergent. Races previously determined using differential cultivars did not correlate with the results... Tipo: Info:eu-repo/semantics/article Palavras-chave: Phaseolus vulgaris; Common bean; DNA; Molecular characterization; Variability. Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582006000600002 Molecular and pathogenic variability of Drechslera isolates from oats Trop. Plant Pathol. MEHTA,Y. R.. Severe epidemics of leaf blotch and black leaf spot of oat (Avena sativa) caused by Drechslera avenae and Drechslera sp., respectively, are frequently observed in the State of Paraná, Brazil. Although some morphological differences between the isolates causing two different symptoms were noticed, the genetic relationship between them was not clear. Twenty-four isolates of D. avenae and Drechslera sp, collected between 1996-98, were assessed for the genetic variability by molecular and pathogenic analyses. The amplification products using primer pair ITS4/ITS5 showed a fragment length of approximately 600 bp for all the isolates except for one black spot isolate, where the fragment length was approximately 550 bp. Restriction enzymes Hinf I and Taq I, that... Tipo: Info:eu-repo/semantics/article Palavras-chave: Pyrenophora avenae; Avena sativa; Molecular characterization; Varietal resistance. Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582001000300002 Registros recuperados: 38 Primeira ... 1 2 ... Última

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