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| Registros recuperados: 38 | |
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| GUIZADO,Stalin Juan Vasquez; NADEEM,Muhammad Azhar; ALI,Fawad; BARUT,Muzaffer; HABYARIMANA,Ephrem; GÓMEZ,Tedi Pacheco; SANTILLAN,Jhon Antoni Vargas; CANALES,Esperanza Torres; GÓMEZ,Juan Carlos Castro; CHUNG,Gyuhwa; BALOCH,Faheem Shehzad. |
| ABSTRACT Rosewood, Aniba rosaeodora is an endangered species in Amazon forests and its natural stands have been heavily depleted due to over-exploitation for the cosmetic industry. This study aimed to investigate the genetic diversity and population structure of 90 rosewood accessions from eight localities in the Peruvian Amazon through 11 Inter Simple Sequence Repeats (ISSR) primers. The ISSR primers produced a sum of 378 bands, of which 375 (99.2%) were polymorphic, with an average polymorphism information content (PIC) value of 0.774. The mean effective number of alleles (Ne), Shannon informative index (I), gene diversity (He) and total gene diversity (Ht) were 1.485, 0.294, 0.453 and 0.252, respectively. Analysis of molecular variance (AMOVA) showed... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aniba rosaeodora; Endangered species; Gene flow; Germplasm; Molecular characterization. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672020000300204 |
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| de Breuil,S; La Rossa,F.R; Giudici,A; Wulff,A; Bejerman,N; Giolitti,F; Lenardon,S. |
| Groundnut ringspot virus (GRSV), genus Tospovirus, is a thrips-transmitted virus infecting peanuts (Arachis hypogaea L.) in Córdoba province, Argentina. Fourteen viral isolates were recovered from Tospovirus-like symptomatic plants from different peanut fields. Viral isolates as GRSV were identified by serological and molecular tests. Nucleotide and derived amino acid sequence analyses of the nucleocapsid (N) gene indicated a high degree of identity between the GRSV peanut isolates, indicating that there is no molecular variability in the N gene of the GRSV that infects peanuts in the cropping area of Córdoba. In this study, we determined the presence of thrips species in the crop, which can potentially transmit the virus. Thrips were observed in all the... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Arachis hypogaea; Tospovirus; Molecular characterization; Frankliniella schultzei. |
| Ano: 2015 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1668-298X2015000100007 |
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| Cury,Gisele Gentile; Mobilon,Cristiane; Stehling,Eliana Guedes; Lancellotti,Marcelo; Ramos,Marcelo de Carvalho; Martinez,Roberto; Brocchi,Marcelo; Silveira,Wanderley Dias da. |
| One hundred and fifty-one methicillin-resistant z (MRSA) strains have been isolated from patients admitted in tertiary care hospitals in two metropolitan areas (Campinas City and Ribeirão Preto City) in the southeast region of Brazil and analyzed through PCR-based techniques [(PCR amplification of spa, coa, and housekeeping genes (arcC, aroE, gmk, pta, tpi, yqiL)] and further restriction fragment typing of coa and of housekeeping genes. The heterogeneity of spa gene was determined directly by agarose gel electrophoresis migration. The results obtained indicate the existence of three (A, B, C) main clusters. Since the strain distribution in these three clusters is much characteristic, it denotes the existence of three main clones. All strains isolated in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: MRSA; Nosocomial; Molecular characterization; PCR. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702009000300002 |
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| Angelini,Michelle; Silveira,Wanderley Dias da; Campos,Tatiana Amabile de; Cai,Silvana; Castro,Antonio Fernando Pestana de. |
| Streptococcus ssp are important components of the dental biofilm and Streptococcus crista is considered to be an interesting model of bacterial interactions taking place in this biofilm. In the present work, S. crista strains were isolated from the dental biofilm of Brazilian individuals and studied with respect to their biological characteristics and their molecular profile by means of AP-PCR techniques, using the RR2, 434, OPR2, OPR8, and OPR13 primers. Results allowed us to build a similarity dendrogram. Analysis of the similarity dendrogram allowed the separation of the studied strains into similarity groups. All isolates presented fibril tufts by Transmission Electron Microscopy (TEM). These isolates were able to bind to salivary amylase and to adhere... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Biological characterization; Molecular characterization; Dendrogram analysis; Biofilm. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000200005 |
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| Puentes,R; Eliopulos,N; Pérez,R; Franco,G; Sosa,K; Bianchi,P; Furtado,A; Hübner,S.O.; Esteves,P.A.. |
| Canine parvovirus type 2 (CPV-2) is a leading cause of diarrhea in puppies in several parts of the world. In this study CPV-2 was detected and recovered from puppies showing clinical disease from Montevideo, Uruguay. Samples were processed and used to infect CRFK and MDCK cells in order to isolate the virus. Out of twelve, two samples were positive for CPV-2. A genomic region of 583 bp was amplified and the molecular characterization was performed by sequencing, phylogenetic analysis and Restriction Fragment Length Polymorphism (RFLP). Two isolated viruses (UY1 and UY2) were CPV-2c-like viruses. The comparison between the cytophatic effect (CPE) of CPV-2 (vaccinal virus) and CPV-2c (isolated virus) on primary canine cells cultures and on CRFK line cells,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Canine Parvovirus 2c; Isolation; Molecular characterization; South America. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300022 |
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| Oliveira,Rosângela C.; Yamaguti,Maurício; Marques,Lucas M.; Buzinhani,Melissa; Rizzo,Huber; Meira-Junior,Enoch Brandão de Souza; Guimarães,Ana Márcia S.; Gregory,Lilian; Timenetsky,Jorge. |
| Abstract Ovine/caprine ureaplasmas have not yet been assigned a species designation, but they have been classified into nine serotypes. Herein ureaplasmas were searched for in 120 samples of vulvo vaginal mucous from sheep and 98 samples from goats at 17 farms. In addition, semen samples were collected from 11 sheep and 23 goats. The recovered ureaplasma were from sheep and goats from animals without any reproductive disorder symptoms, but not all animals presented positive cultures. In sheep, 17 (68%) cultures of vulvovaginal mucous were positive for ureaplasma and 11 (27%) samples of semen presented positive cultures in animals with clinical signs of orchitis, balanoposthitis or low sperm motility. In goats four ureaplasma isolates were obtained from... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ureaplasma; Ovine; Caprine; Molecular characterization. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000300560 |
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| Morillo,Simone Guadagnucci; Audrey,Cilli; Carmona,Rita de Cássia Compagnoli; Timenetsky,Maria do Carmo Sampaio Tavares. |
| Norovirus (NoV), previously called Norwalk-like virus, represents an important group of human pathogens associated with outbreaks of nonbacterial gastroenteritis. Epidemiological surveys of outbreaks have shown that the most important routes of transmission are person-to-person contacts and contaminated food and water, with the virus affecting adults and children. NoV is classified into genogroups, being genogroups GI, GII and GIV found in humans. In view of the high genetic diversity of NoV and the lack of information about this virus in Brazil, the aim of the present study was the molecular characterization of NoV isolated from diarrheic stool samples of patients from São Paulo State. In this study, 204 stool specimens collected during diarrhea outbreaks... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Norovirus; Genogroup GII; Molecular characterization. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000400004 |
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| Hou,Xiang-hua; Song,Xiu-yu; Ma,Xiao-bo; Zhang,Shi-yang; Zhang,Jia-qin. |
| Klebsiella pneumoniae is an important cause of healthcare-associated infections worldwide. Selective pressure, the extensive use of antibiotics, and the conjugational transmission of antibiotic resistance genes across bacterial species and genera facilitate the emergence of multidrug-resistant (MDR) K. pneumoniae. Here, we examined the occurrence, phenotypes and genetic features of MDR K. pneumoniae isolated from patients in intensive care units (ICUs) at the First Affiliated Hospital of Xiamen University in Xiamen, China, from January to December 2011. Thirty-eight MDR K. pneumoniae strains were collected. These MDR K. pneumoniae isolates possessed at least seven antibiotic resistance determinants, which contribute to the high-level resistance of these... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Klebsiella pneumoniae; Multidrug resistance; Molecular characterization. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000300759 |
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| Paula,Márcia Beatriz Cardoso de; Yokosawa,Jonny; Coutinho,Márcio Danilo Botrel; Silva,Paulo Lourenço; Ferraz,Rogério Avelar; Oliveira,Thelma Fátima Mattos; Queiróz,Divina Aparecida Oliveira. |
| In order to identify and characterize the agent of a suggestive clinical case of Gumboro disease (GD) that affected a 34-day-old broiler flock in Buriti Alegre (Goias State, Midwestern Brazil) in the year 2001, we carried out a combination of classic and modern virological methods. Histopathological analysis of the bursa revealed necrosis, presence of depleted follicles, some infiltration of heterophils, edema and formation of cystic cavities that are compatible with lesions observed in GD. Inoculation of embryonated eggs of specific pathogen-free (SPF) chickens with macerated bursa suspension resulted in embryo mortality and lesions which were also compatible with those caused by IBDV. A sample of bursa was submitted to a nested reverse... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chicken broiler flock; Gumboro disease; Infectious bursal disease virus; IBDV; RT-PCR; Molecular characterization. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000300016 |
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| Pereira,Joylson de Jesus; Baumworcel,Natasha; Fioretti,Júlia Monassa; Domingues,Cinthya Fonseca; Moraes,Laís Fernandes de; Marinho,Robson dos Santos Souza; Vieira,Maria Clara Rodrigues; Pinto,Ana Maria Viana; de Castro,Tatiana Xavier. |
| ABSTRACT The aim of this study was to perform the molecular characterization of conserved and variable regions of feline calicivirus capsid genome in order to investigate the molecular diversity of variants in Brazilian cat population. Twenty-six conjunctival samples from cats living in five public short-term animal shelters and three multicat life-long households were analyzed. Fifteen cats had conjunctivitis, three had oral ulceration, eight had respiratory signs (cough, sneeze and nasal discharge) and nine were asymptomatic. Feline calicivirus were isolated in CRFK cells and characterized by reverse transcription PCR target to both conserved and variable regions of open reading frame 2. The amplicons obtained were sequenced. A phylogenetic analysis... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Conjunctivitis; FCV genogroup; Household; Molecular characterization; Shelter. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000400777 |
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| Caleffi-Ferracioli,Katiany Rizzieri; Baldin,Vanessa Pietrowski; Noguti,Érika Noda; Siqueira,Vera Lucia Dias; Scodro,Regiane Bertin Lima; Pavan,Fernando Rogério; Hirata,Mario Hiroyuki; Cardoso,Rosilene Fressatti. |
| Tuberculosis (TB) is an infectious disease in which the molecular typing methods allow to have important information about the dynamics of transmission and to assist properly in disease control. Although the ERIC-PCR (Enterobacterial repetitive intergenic consensus-PCR) assay is fast and easy to perform, scarce studies have reported its use in epidemiological studies in TB outbreaks. In this study, we aimed to genotype Mycobacterium tuberculosis and M. bovis isolates by ERIC-PCR and compare its discriminatory power with two other classically used methods: 12 loci-MIRU (Mycobacterial Interspersed Repetitive Units) and Spoligotyping. The M. tuberculosis isolates studied were from northwestern and southwestern and M. bovis from northwestern Parana, Brazil.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Tuberculosis; Molecular characterization; ERIC-PCR; Epidemiology. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000300616 |
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| Besoain,Ximena; Bertolini,Edson; Camps,Rocío; Ramella,Fiona; Gorris,María Teresa; Torres,Margarita; Cambra,Mariano. |
| Citrus tristeza virus (CTV) was reported in the 1960's to affect Meyer lemon trees in Chile, but no field symptoms were observed. This study performed a complete biological, serological and molecular characterization of one hundred CTV isolates obtained from different hosts and geographical origins. Decline symptoms (DI) were not found on trees grafted onto sour orange. In Pica Oasis in northern Chile, stem pitting (SP) was found to affect grapefruit and Mexican lime trees. Most isolates present in the central area were considered mild (MCA-13-negative), while in the northern area aggressive isolates were observed and detected. Some of these isolates were capable of causing SP on grapefruit under field and greenhouse conditions and on sweet orange under... |
| Tipo: Journal article |
Palavras-chave: Biological indexing; CTV; Molecular characterization; Stem pitting; Seedling yellows. |
| Ano: 2015 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202015000200011 |
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| Sudré,Cláudia Pombo; Rodrigues,Rosana; Gonçalves,Leandro Simões Azeredo; Martins,Ernane Ronie; Pereira,Messias Gonzaga; Santos,Marilene Hilma dos. |
| The genus Dimorphandra has distinguish relevance considering either medicinal or biodiversity aspects because it includes two species that are economically important flavonoids sources for pharmachemical industry (D. mollis Benth. and D. gardneriana Tul.), and species endemic to Brazil, such as D. jorgei Silva and D. wilsonii Rizz., threatened by extinction. In order to evaluate variability among accessions of D. mollis (fava-d'anta), D. gardneriana and D. wilsonii, it was collected fruit from individual plants from three Brazilian states in a total of 57 accessions, which were analyzed with RAPD markers. It was used 20 seeds per progeny; the DNA was extracted from fully-formed young leaves, which were collected in bulk. The data were analyzed using a... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Fava-d'ant; Molecular characterization; Plant genetic resource; Germoplasm. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782011000400010 |
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| Jordan Aguilar, Bladimir. |
| En México el haba (Vicia faba L.) es importante para la alimentación de los habitantes del medio rural y urbano, contiene un alto contenido nutritivo, es un alimento balanceado, asimismo en la actualidad los productores demandan variedades con mayor calidad de semilla. El rango en cuanto al contenido de taninos en las semillas de haba cultivadas por los agricultores fue determinado, se estimaron las relaciones entre las variedades de haba mediante la utilización de marcadores moleculares tipo RAPD y microsatélites y se propone una estrategia de aprovechamiento de las variedades por los productores de haba con base a los caracteres estudiados. El desarrollo de este estudio se realizó en dos etapas, la primera consistió en la determinación del contenido de... |
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Palavras-chave: Taninos; Caracterización molecular; Variedades de haba; Líneas estratégicas; Tannin; Molecular characterization; Varieties of faba bean; Strategy lines. |
| Ano: 2011 |
URL: http://hdl.handle.net/10521/438 |
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| Registros recuperados: 38 | |
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