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| Registros recuperados: 28 | |
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| Galicia-García,Porfirio Raúl; Silva-Rojas,Hilda Victoria; Mendoza-Onofre,Leopoldo Ernesto; Zavaleta-Mancera,Hilda Araceli; Córdova-Téllez,Leobigildo; Espinosa-Calderón,Alejandro. |
| ABSTRACT Ustilago maydis is a basidiomycete known as the causative agent of 'common smut', worldwide disease of maize that is recognized by the galls it forms, which have considerable potential as a gourmet food. Results of infection are quite variable, even under optimal greenhouse conditions. In order to find pathogenic strains able to be used as a highly infective and stable inoculum for the successful production of galls either in greenhouses or in the field, ears with gall symptoms containing teliospores were recovered from maize plants. The teliospores were suspended in water and plated on nutrient-rich medium. Twenty-six colonies developed, containing three types of yeast-like colonies: saprotrophic, pathogenic, and solopathogenic. DAPI staining... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Basidiomycete; Maize galls; Mating types; Molecular diagnosis; Smut. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062016000400683 |
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| Cerqueira Melo, Rita de Cássia; Dorea Bragança, Carlos Augusto; Nogueira Pestana, Katia; Silva, Harllen Sandro Alves da; Ferreira, Claudia Fortes; Alves Santos de Oliveira, Saulo. |
| Modifications were made in the PCR conditions aiming to overcome the problem of non-amplification of the Xanthomonas phaseoli pv. manihotis (Xpm) fragment, using the primer pair XV / XK described in the literature. The objective of this study was to propose changes in the primers already described (XV / XK_MOD) and validate the use of these new primers in identifying Xpm. The validation procedure was carried out with the primer pair XV and XK_MOD, using different strains of Xpm, other plant pathogenic and endophytic bacteria genera and one isolate of X. phaseoli pv. passiflorae. As a control, additional reactions were conducted in multiplex with the universal primers for the 16S rRNA gene of the bacteria together with XV / XK and XV / XK_MOD. Using the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Xpm; Cassava bacterial blight; Molecular diagnosis; Resistance. |
| Ano: 2019 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/42708 |
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| Costa,R.L.; Vitari,G.L.V.; Silva,C.B.; Peckle,M.P.; Pires,M.S.; Brandolini,S.V.P.B.; Pinheiro,J.; Massard,C.L.; Santos,H.A.. |
| ABSTRACT In Brazil, some studies have indicated that Neorickettsia risticii circulates in horses, but it is unclear which are the possible intermediate vectors of this bacterium in the country. The aim of this study was to use molecular techniques in order to analyze the presence of N. risticii in snails and larval stages of trematodes in farms in a region with a history of seroreactive horses towards this bacterium, in Rio de Janeiro, Brazil. Convenience sampling was used in the studied region. The collected snails were exposed to incandescent light (60W) for 2-4 hours in order to investigate trematodes in larval forms. Deoxyribonucleic acid (DNA) was extracted from snail tissue and trematode. Real-time PCR (qPCR) technique was used to investigate the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Equine neorickettsiosis; Molecular diagnosis; QPCR; Intermediate hosts. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352016000601470 |
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| Thomaz-Soccol,Andréa; Mocellin,Marcos; Mulinari,Fabiane; Castro,Edilene Alcântara de; Queiroz-Telles,Flávio de; Alcântara,Fernanda de Souza; Bavaresco,Marcello Tortelli; Hennig,Luciane; Andraus,Anielo; Luz,Ennio; Thomaz-Soccol,Vanete. |
| The aim of the present work was to study the clinical aspects and relevance of molecular diagnosis in late mucocutaneous leishmaniasis patients in Parana, Brazil. Twenty one suspected cases of mucocutaneous leishmaniasis (MCL) in patients from the endemic areas of leishmaniasis were assessed. Different methods used in diagnosing the disease and the polymerase chain reaction (PCR) technique were compared in order to establish the sensitivity of each method. Out of the 21 patients analyzed, 14.3% presented other etiologies such as vasculitis, syphilis, and paracoccidioidomycosis, with all tests negative for leishmaniasis. Out of the remaining 15 patients, 6.7% cases were confirmed for leishmaniasis by direct examination; 46.67% were positive for culture,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mucocutaneous leishmaniasis; Molecular diagnosis; Leishmania (Viannia) braziliensis. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000300008 |
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| Quiles,Milene Gonçalves; Boettger,Bruno Cruz; Inoue,Fernanda Matsiko; Monteiro,Jussimara; Santos,Daniel Wagner; Ponzio,Vinicius; Carlesse,Fabianne; Cappellano,Paola; Carvalhaes,Cecilia Godoy; Pignatari,Antonio Carlos Campos. |
| ABSTRACT Bloodstream infections (BSIs) are serious infections associated with high rates of morbidity and mortality. Every hour delay in initiation of an effective antibiotic increases mortality due to sepsis by 7%. Turnaround time (TAT) for conventional blood cultures takes 48 h, forcing physicians to streamline therapy by exposing patients to broad-spectrum antimicrobials. Our objective was (1) to evaluate the accuracy and TAT of an optimized workflow combining direct matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) and in-house real-time polymerase chain reaction (PCR) for bacterial identification and antimicrobial resistance profiling directly from positive blood bottles for diagnosing bloodstream infections... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bloodstream infections; Molecular diagnosis; Molecular panels; PCR; Mass spectrometry. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702019000300164 |
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| Hung,Hung-Chang; Chan,Chi-Ho; Tsao,Shih-Ming; Chen,Shiuan-Chih; Wu,Tzu-Chin; Lu,Min-Chi; Wu,Der-Jinn; Huang,Shu-Ju; Lee,Yuan-Ti. |
| OBJECTIVE: The diagnostic efficacy of the BDProbeTEC ET Mycobacterium tuberculosis (MTB) complex direct detection assay (DTB) performed on bronchoalveolar lavage (BAL) specimens and sputum smears was compared with acid-fast bacilli (AFB) smear microscopy. METHOD: AFB smear microscopy, DTB and culture results of 286 patients with pulmonary tuberculosis were retrospectively reviewed. A total of 120 patients provided expectorated sputum samples, and 166 patients provided BAL specimens. Culture results and clinical diagnosis were used as gold standards. RESULTS: The sensitivity and specificity of the DTB assay in detecting MTB in sputum specimens was significantly higher compared to AFB smear microscopy (83.7% and 82.4%, vs. 75.6%, and 41.2%, respectively).... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bronchoalveolar lavage; Mycobacterium tuberculosis; Acid-fast bacilli staining; Molecular diagnosis; BDProbeTEC ET system. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702012000300005 |
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| Rivera,Vanessa; Gaviria,Marcela; Muñoz-Cadavid,Cesar; Cano,Luz; Naranjo,Tonny. |
| ABSTRACT The diagnosis of cryptococcosis is usually performed based on cultures of tissue or body fluids and isolation of the fungus, but this method may require several days. Direct microscopic examination, although rapid, is relatively insensitive. Biochemical and immunodiagnostic rapid tests are also used. However, all of these methods have limitations that may hinder final diagnosis. The increasing incidence of fungal infections has focused attention on tools for rapid and accurate diagnosis using molecular biological techniques. Currently, PCR-based methods, particularly nested, multiplex and real-time PCR, provide both high sensitivity and specificity. In the present study, we evaluated a nested PCR targeting the gene encoding the ITS-1 and ITS-2... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cryptococcus neoformans/Cryptococcus gattiicomplex; Nested PCR; Cryptococcosis; Molecular diagnosis. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702015000600563 |
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| Sepúlveda,Lya Madureira; Malirat,Viviana; Bergmann,Ingrid E.; Mantilla,Anibal; Nascimento,Elmiro Rosendo do. |
| Vesicular Stomatitis (VS) is a viral disease that has a great impact in animal health, as infected animals present marked decrease in meat and milk production. Its presence is a limiting factor for international animal trade. Besides the damage in the livestock productivity, such disease assumes an important role in animal health programs since it is clinically indistinguishable from Foot-and-Mouth Disease. The diagnosis of the VS has been made, mainly, through Complement Fixation, ELISA and Virus Neutralization tests, assays that allow not only for viral detection but also for differentiation of the two serotypes described for Vesicular Stomatitis Virus (VSV): New Jersey (NJ) and Indiana (Ind). In this work, a molecular diagnostic approach, the polymerase... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Polymerase Chain reaction; Vesicular Stomatitis; Molecular diagnosis. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000300022 |
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| Mares-Guia,Maria Angélica M.M.; Guterres,Alexandro; Rozental,Tatiana; Ferreira,Michelle dos Santos; Lemos,Elba R.S.. |
| ABSTRACT Q fever is a worldwide zoonosis caused by Coxiella burnetii—a small obligate intracellular Gram-negative bacterium found in a variety of animals. It is transmitted to humans by inhalation of contaminated aerosols from urine, feces, milk, amniotic fluid, placenta, abortion products, wool, and rarely by ingestion of raw milk from infected animals. Nested PCR can improve the sensitivity and specificity of testing while offering a suitable amplicon size for sequencing. Serial dilutions were performed tenfold to test the limit of detection, and the result was 10× detection of C. burnetti DNA with internal nested PCR primers relative to trans-PCR. Different biological samples were tested and identified only in nested PCR. This demonstrates the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Q fever; Coxiella burnetii; Molecular diagnosis; Nested PCR; IS1111. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000100138 |
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| Silva,S.E.L. da; Bonetti,A.M.; Petrocelli,A.; Ferrari,H.F.; Luvizotto,M.C.R.; Cardoso,T.C.. |
| A survey of Turkey Coronavirus (TCoV) and Astrovirus (TAstV-2) prevalence was carried out from February to December during 2006 year in semiarid region of Brazil, from a turkey producer area, localized in South Eastern of Brazil. To asses the risk factor related to clinical material, climatic condition and type of RT-PCR applied, cloacal swabs (CS), faeces, sera, bursa of Fabricius (BF), thymus (TH) and spleen (SP) and ileum-caeca region were collected from 30-day-old poults suffering of enteritis episode characterized as poult enteritis mortality syndrome (PEMS). The PEMS clinical features were characterized by watery to foamy faeces, light brown-yellow in colour and low mortality rate. Meteorological data (rainfall and relative humidity) observed during... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Turkey Astrovirus; Turkey Coronavirus; Molecular diagnosis; PEMS. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000200008 |
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| Cruz,Heidi Lacerda Alves da; Montenegro,Rosana de Albuquerque; Lima,Juliana Falcão de Araújo; Poroca,Diogo da Rocha; Lima,Juliana Figueirêdo da Costa; Montenegro,Lílian Maria Lapa; Crovella,Sergio; Schindler,Haiana Charifker. |
| The polymerase chain reaction (PCR) and its variations, such as the nested-PCR, have been described as promising techniques for rapid diagnosis of tuberculosis (TB). With the aim of evaluating the usefulness of a nested-PCR method on samples of blood and urine of patients suspected of tuberculosis we analyzed 192 clinical samples, using as a molecular target the insertion element IS6110 specific of M. tuberculosis genome. Nested-PCR method showed higher sensitivity in patients with extrapulmonary tuberculosis (47.8% and 52% in blood and urine) when compared to patients with the pulmonary form of the disease (sensitivity of 29% and 26.9% in blood and urine), regardless of the type of biological sample used. The nested-PCR is a rapid technique that, even if... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mycobacterium tuberculosis; Nested-PCR; Blood; Urine; Molecular diagnosis; IS6110 insertion sequence. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100041 |
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| Bianchi,Ronaldo Michel; Cecco,Bianca Santana de; Schwertz,Claiton Ismael; Panziera,Welden; Andrade,Caroline Pinto de; Spanamberg,Andreia; Ravazzolo,Ana Paula; Ferreiro,Laerte; Driemeier,David. |
| ABSTRACT: This report describes the clinical, pathological, and molecular aspects of a pneumonia by Cryptococcus neoformans in a goat in the Southern region of Brazil. A goat with a history of prolonged transportation presented dyspnea, nasal discharge and cough, and was subjected to necropsy, histopathology, and mycological evaluation. Grossly, cranio-ventral pulmonary consolidation was observed, characterized by firm and grayish areas interspersed with dark red foci. Histopathology of the lungs showed areas of parenchymal necrosis, containing blastoconidia with a slightly basophilic central cell, surrounded by an unstained capsule. It was associated with moderate granulomatous inflammatory infiltrate and peripheral fibrosis. The capsule and fungus cell... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Goats; Respiratory diseases; Pathology; Mycology; Molecular diagnosis. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018001000551 |
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| Bianchi,Ronaldo Michel; Vielmo,Andréia; Schwertz,Claiton Ismael; Cabreira,Adan; Rocha,Jéssica Goulart da; Siqueira,Franciele Maboni; Pavarini,Saulo Petinatti. |
| ABSTRACT: This study described the pathological and microbiological aspects of a fatal systemic Mortierella wolfii infection in a neonatal calf in southern Brazil. The calf was born clinically normal, but on the third day of life it presented apathy, unilateral hypopyon, and neurological signs, and in the next day it was euthanized. At necropsy, multiple soft, and white-yellow nodules were observed in the liver, spleen, kidneys, mesenteric lymph nodes, heart, and lungs. In the brain, reddish, and friable areas were found. In the eye, there were anterior and posterior synechiae, diffuse thickening of choroid, and anterior chamber filled by whitish and friable material. Microscopically, areas of necrosis, pyogranulomatous inflammation, vasculitis,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cattle diseases; Fungal diseases; Pathology; Molecular diagnosis. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782020000100551 |
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| Ferreira,Maiara Sanitá Tafner; Vogel,Fernanda Silveira Flores; Sangioni,Luis Antonio; Weber,Augusto; Bräunig,Patricia; Vaz,Marcos André Braz; Cezar,Alfredo Skrebsky. |
| ABSTRACT: Neosporosis is a parasitic disease caused by the protozoan Neospora caninum which results in major economic losses for cattle breeding due to abortion and other reproductive disorders. Gerbils (Meriones unguiculatus) are commonly used as experimental models for neosporosis due to their high susceptibility to N. caninum infection, both by oocysts ingestion as by tachyzoites/bradyzoites parenteral inoculation. However, the risk of transmission by tachyzoites ingestion is not fully elucidated. In this study, infection of neonate gerbils by N. caninum (NC-1 strain) tachyzoites inoculated by the oral route and the parasite distribution in gerbils' tissues were evaluated by protozoan DNA detection. Seventeen neonate gerbils, aged 4-5 days, were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Molecular diagnosis; Experimental model; Bovine neosporosis; Rodents. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000400654 |
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| Registros recuperados: 28 | |
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