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| Brogni,Cláudio Francisco; Ohlweiler,Lain Uriel; Klein,Norton; Mezzalira,Joana Claudia; Cristani,Jose; Mezzalira,Alceu. |
| ABSTRACT: The main cause of low efficiency of in vitro produced porcine embryos is the high polyspermic penetration rates at fertilization, which is aggravated in low quality oocytes. Experiment 1 evaluated the embryo development in high and low quality oocytes. Experiment 2 evaluated the embryo development and quality of low quality oocytes fertilized with sperm pre-incubated during 0h (control), 0.5h, 1h and 1.5h. Experiment 3 investigated fertilization and monospermic rates of the same groups of Experiment 2. Experiment 4 evaluated embryo development, cell density, fertilization and monospermic rates of high quality oocytes using semen pre incubated during the best time observed in the previous experiments. Cleavage and blastocyst rates were analyzed by... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pig IVF; Embryo IVP; Monospermy; Fertilization; Oocyte. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000601113 |
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