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Biostimulation of inoculation with Glomus proliferum and application of humic acid in the in vitro growth of Lunularia cruciata Acta Botanica
Nobre,Camila Pinheiro; Huertas,Orlando Carlos Tavares; Tardin,José Rogério Figueira; Saggin Júnior,Orivaldo José; Fonseca,Henrique Manoel Apolónia Coutinho; Berbara,Ricardo Luis Louro.
In this study, we evaluated the growth of the liverwort Lunularia cruciata, inoculated or not with the arbuscular mycorrhizal fungi (AMF) Glomus proliferum (15 spores per Petri dish), in Strullu-Romand Variant (SRV) medium modified and enriched with humic acid (HA) at different concentrations (0, 20, 40 and 80 mg C L−1), as well as the sporulation of the AMF. We assessed the absolute growth rate (AGR) and relative growth rate (RGR) at inoculation, as well as at 7, 14, 21, 28, 43, 52 and 60 days after inoculation (DAI), whereas we assessed sporulation at 25, 43, 60 and 70 DAI. The main determinant of L. cruciata growth was the presence of AMF. With and without G. proliferum inoculation, respectively, the AGR peaked at 39 and 42 DAI, and the RGR was 0.0474...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Arbuscular mycorrhizal fungi; Liverworts; Monoxenic cultures; Humic substances.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062013000400017
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Dominance of Paris-type morphology on mycothallus of Lunularia cruciata colonised by Glomus proliferum BJM
Fonseca,Henrique M.A.C.; Ferreira,Joana I.L.; Berbara,Ricardo L.L.; Zatorre,Natalia P..
Microscopic evidence confirms that L. cruciata hosting G. proliferum shows major anatomical traits (arbuscules, coils, arbusculate coils and vesicles) generally associated arbuscular mycorrhizal roots and the anatomical morphology of intra-thalli mycelium is predominantly of the Paris-type. Colonised L. cruciata showed a reduction of biomass when compared with axenic plants suggesting a drain of resources towards the fungus and depletion of nutrients required for optimum plant growth. The behaviour of mycothalli regarding available KH2PO4 indicates that the nutritional stress threshold for phosphorus (P) is above the residual amount of P already present in PhytagelTM and in plant inoculum. These raise the possibility that in certain circumstances the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Arbuscular mycorrhizal fungi; Phosphorus; Arum-type; Liverwort; Monoxenic cultures.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000100016
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