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| Marques,K.B.; Scorisa,J.M.; Zanon,R.; Freria,C.M.; Santos,L.M.B.; Damasceno,B.P.; Oliveira,A.L.R.. |
| The immunomodulador glatiramer acetate (GA) has been shown to significantly reduce the severity of symptoms during the course of multiple sclerosis and in its animal model - experimental autoimmune encephalomyelitis (EAE). Since GA may influence the response of non-neuronal cells in the spinal cord, it is possible that, to some extent, this drug affects the synaptic changes induced during the exacerbation of EAE. In the present study, we investigated whether GA has a positive influence on the loss of inputs to the motoneurons during the course of EAE in rats. Lewis rats were subjected to EAE associated with GA or placebo treatment. The animals were sacrificed after 15 days of treatment and the spinal cords processed for immunohistochemical analysis and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Experimental autoimmune encephalomyelitis; Spinal cord; Synapse elimination; Motoneuron; Immunomodulator; Glatiramer acetate. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000200006 |
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| Rezende,A.C.S.; Vieira,A.S.; Rogério,F.; Rezende,L.F.; Boschero,A.C.; Negro,A.; Langone,F.. |
| Ciliary neurotrophic factor (CNTF) is a cytokine that plays a neuroprotective role in relation to axotomized motoneurons. We determined the effect of daily subcutaneous doses of CNTF (1.2 µg/g for 5 days; N = 13) or PBS (N = 13) on the levels of mRNA for Bcl-2 and Bax, as well as the expression and inter-association of Bcl-2 and Bax proteins, and the survival of motoneurons in the spinal cord lumbar enlargement of 2-day-old Wistar rats after sciatic nerve transection. Five days after transection, the effects were evaluated on histological and molecular levels using Nissl staining, immunoprecipitation, Western blot analysis, and reverse transcriptase-polymerase chain reaction. The motoneuron survival ratio, defined as the ratio between the number of... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Ciliary neurotrophic factor; Bax; Neonatal rats; Axotomy; Motoneuron; Immunoprecipitation. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008001100013 |
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