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| Picazo,María G; García-Olmo,Dolores C. |
| Background Genotyping of mice is a common procedure in animal facilities. The aim of this study was to compare the quantity and quality of DNA extracted from samples obtained from young mice (YM; 10 d old) and adult mice (AM; 12 weeks old). We collected samples from the tail and ear of YM and AM. We also sampled blood, check cells (via buccal swabs), hair and fecal pellets of AM, and biopsied distal phalanx of YM. We isolated DNA using commercial kits and determined concentrations and purity by spectrophotometry. The integrity of DNA was evaluated by agarose-gel electrophoresis and polymerase chain reaction (PCR). Results DNA in all samples was amplified successfully but the intensities of bands after electrophoresis was heterogeneous. In general, tissues... |
| Tipo: Journal article |
Palavras-chave: DNA extraction; Genotyping; Mouse. |
| Ano: 2015 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000200004 |
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| Bakare,Adekunle A.; Okunola,Alabi A.; Adetunji,Olusanmi A.; Jenmi,Hafeez B.. |
| Pharmaceutical industries are among the major contributors to industrial waste. Their effluents when wrongly handled and disposed of endanger both human and environmental health. In this study, we investigated the potential genotoxicity of a pharmaceutical effluent, by using the Allium cepa, mouse- sperm morphology, bone marrow chromosome aberration (CA) and micronucleus (MN) assays. Some of the physico-chemical properties of the effluent were also determined. The A. cepa and the animal assays were respectively carried out at concentrations of 0.5, 1, 2.5, 5 and 10%; and 1, 5, 10, 25 and 50% of the effluent. There was a statistically different (p < 0.05), concentration-dependent inhibition of onion root growth and mitotic index, and induction of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Genotoxicity; Pharmaceutical effluent; Mouse; Allium cepa; Chromosome; Spermatozoa; Micronucleus. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200026 |
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| Bustos-Obregón,Eduardo; Poblete,Daniel; Catriao,Roberto; Fernandes,Fábio Henrique. |
| Arsenic is a testicular environmental toxic. Melatonin (Me), being a potent antioxidant, may reduce the damage caused by arsenic in male fertility. The effects of daily oral exposure of Sodium Arsenite (As; 7.0 mg/kg/bw); Melatonin (Me, 10.0 mg/kg/bw); Me (10.0 mg/kg/bw) plus As (7.0 mg/kg/bw), and Negative Control (NaCl 0.9%) in male CF-1 adult mice were assessed in acute (8.3 days), chronic (33.2 days) and recovery (66,4 days) of testicular damage. We evaluated changes in testicular weight and histopathological, morphometric measurements, expression of COX-2 and Androgen Receptor (AR) antigens and lipid peroxidation levels. Treatment resulted in decreased tubular diameter and AR expression, and increased: interstitial area, luminal diameter, COX-2... |
| Tipo: Journal article |
Palavras-chave: Mouse; Arsenic; COX-2; Androgen receptor; Oxidative stress; Melatonin. |
| Ano: 2013 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000300012 |
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| Catena,M; Teruel,M; Morán,P; Chiapparrone,M; Echevarria,H; Soto,P. |
| Bovine campylobacteriosis caused by Campylobacter fetus is associated with reproductive losses. The knowledge about the mechanisms of bacterial pathogenesis is limited, then a murine experimental model is proposed. BALB/c females and males were used. Two-cell embryos were cultured in Ham-F10 as control group (CG). Treatment groups were constituted by the addition of Cfv 1 and 3, or Cff 2 and 5. Morulae were placed in Ham-F10 (CG); treatment groups were constituted by the addition of Cfv27, CFF (cell-free filtrate) and Brucella broth (BB). Blastocysts were cultured in MEM (CG); challenge group were constituted by the addition of Cfv 27. Differentiation, hatching, hatched, adhesion and expansion were evaluated. Results were analyzed by Chi2 test. In two-cell... |
| Tipo: Journal article |
Palavras-chave: Campylobacteriosis; Blastocyst; Morulae; In vitro; Mouse. |
| Ano: 2013 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000400004 |
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| Márquez-Orozco,María Cristina; Gasca-Ramírez,María Verónica; de la Fuente-Juárez,Graciela; Márquez-Orozco,Amalia. |
| Diazepam (DZ) is a benzodiazepine that belongs to the group of minor tranquilizers with myo-relaxing and anticonvulsant properties. DZ and its metabolites cross the placental barrier in human, monkey, hamster, and mouse, and accumulate in the placenta. Our aim was to investigate, through histological techniques, and semifine sections if DZ induces morphological changes in the placenta. Twenty female mice of the ICR strain were distributed randomly in two groups. One group (DZ) was treated from days 6 to 17 of gestation with a single daily subcutaneous (sc) dose of DZ of 2.7 mg/kg/ (bw); the second, control group (C) was treated with saline solution. All females (10 DZ and 10 C) were killed by decapitation. Placentas were extracted and fixed in... |
| Tipo: Journal article |
Palavras-chave: Benzodiazepine; Diazepam; Mouse; Placenta; Histology. |
| Ano: 2014 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022014000200007 |
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| Martins,M. R. F. B; Silva,P. J. R; Martins,B. B; Zoca,S. M; Freitas,M. A; Batista,T. C. |
| In order to study the morphological changes that occur in cells of the testes of isogenic black mouse C57BL/6/Uni into three periods during spermatogenetic used 15 mice divided into 3 groups of 5 animals with 40, 50 and 60 days of age. The mice were sacrificed and weighed. Then weighed and measured the testicles, to be processed finalmete histologically and stained with HE, PAS and Masson Massom-H and evaluated under light microscopy. Was observed in group I with 40 days of age in the seminiferous tubules had a lumen with sparse small amount of interstitial tubular cells. In the seminiferous epithelium were identified type A spermatogonia, intermediate and B, which occupied the compartment adbasal and intermingled with these cells was observed in... |
| Tipo: Journal article |
Palavras-chave: Spermatogenesis; Mouse; Testes. |
| Ano: 2012 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000100052 |
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| Bustos-Obregón,Eduardo; Olivares,Christopher. |
| It has been reported that boron causes changes in various systems, including the male reproductive system. Residents in some towns in northern Chile were consuming a few years ago in the drinking water 20 times more than the amount established as permissible limit by WHO. This study evaluates the effects in an animal model of high intake of boron on the testis. Boron was administered in the drinking water. Twenty male mice (Mus domesticus), sexually mature, were used, divided into 2 groups: the experimental group was given Boron at a dose of 12 mg/L, and the control group 0.6 mg/L, for 42 days. Sections of testis were obtained for: HE staining (Morphometry and Histopathology), Immunohistochemistry (Cox-2), Mallory and Picrosirious stain (evaluation of... |
| Tipo: Journal article |
Palavras-chave: Boron; Mouse; Testicular structure; Collagen I/III; Infertility. |
| Ano: 2012 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000300057 |
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| Freitas,E. S.; Leite,E. D.; Silva,A. E.; Ocarino,N. M.; Ferreira,E.; Gomes,M. G.; Cassali,G. D.; Serakides,R. |
| The main purpose of this study was to investigate the effect of thyroxine and PTU in ascitic Ehrlich tumor cells. Tumor was implanted in 30 female mice distributed in three groups: treated with PTU, treated with thyroxine and control. Each group received an intraperitoneal injection of neoplastic cells, pre-incubated with sterile solutions of PTU, thyroxine and distilled water, respectively. On the fifth and seventh days after inoculation, animals received an intraperitoneal injection of the respective solutions. On the tenth day after inoculation, animals were sacrificed. Volume of ascitic liquid, number of neoplastic cells/ml and percentage of viable cells were determined. Ascitic liquid smears were carried out for tumor cytological evaluation. There was... |
| Tipo: Journal article |
Palavras-chave: Cytology; Ehrlich tumor; Mouse; NORs; Propylthiouracil; Thyroxine. |
| Ano: 2006 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022006000500024 |
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| Espinoza-Navarro,O; Vilaxa,A; Granifo,L; Rojas,S; Rodríguez,H. |
| Boric acid and borate are abundant in the nature and widely used in industrial, agricultural and cosmetology applications. However it is informed as toxic in a high dose. Residents of Arica-Chile with boron-rich geology deposits are regularly exposed to higher levels in drinking water in concentrations of 6. 0 and 12.0 mg/L. The objective of this study was to analyze the damage in the masculine reproductive apparatus of mouse CF1, exposed to boric acid in concentrations of 0.54; 2.0-6.0 and 12.0 mg/L, after 60 days of treatment. Male gonad segments were fixed in Bouin's solution for 6 hours and embedded in paraffin, longitudinal sections (5 mm) were processed for histology using the hematoxyline/eosin method. The results observed in treated animals... |
| Tipo: Journal article |
Palavras-chave: Boric acid; Spermatogenesis; Testicular atrophy; Mouse. |
| Ano: 2007 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022007000200017 |
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| Martins,M. R. F. B; Silva,P. J. R; Martins,B. B; Zoca,S. M; Freitas,M. A; Batista,T. C. |
| In order to study the morphological changes that occur in cells of the testes of isogenic black mouse C57BL/6/Uni into three periods during spermatogenetic used 15 mice divided into 3 groups of 5 animals with 40, 50 and 60 days of age. The mice were sacrificed and weighed. Testicles were weighed and measured, and histologically processed and stained with HE, PAS and Masson Massom-H and evaluated under light microscopy. It was observed that group I with 40 days of age in the seminiferous tubules had a lumen with sparse small amount of interstitial tubular cells. In the seminiferous epithelium type A spermatogonia, intermediate and B were identified, which occupied the compartment adbasal and intermingled with these cells in spermatocytes I in Pachytene and... |
| Tipo: Journal article |
Palavras-chave: Spermatogenesis; Mouse; Testes. |
| Ano: 2012 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000200022 |
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| Teruel,M; Catalano,R; Salomón,L. |
| The aim of this work was to evaluate the effect of albendazole sulphoxide (ABZSO) administered to Balb C mice prior to mating on fertilization rate and preimplantational embryo development. Twenty four female mice 5-8 weeks of age were superovulated by intraperitoneal injection of 7.5 UI of equine chorionic gonadotropin (eCG, Novormon®, Laboratorios Syntex S.A., Argentina); 48 h later they received 10 IU of human chorionic gonadotropin (hCG, Profasi®, Laboratorios Serono, Méjico) and were paired with males of proven fertility. Females received 100 mg/kg or 200 mg/kg of ABZSO orally at the time of hCG administration, prior to mating. The control group received carboxymethylcellulose, vehicle used to prepare the drug suspension. Pregnant females were killed... |
| Tipo: Journal article |
Palavras-chave: Albendazole Sulphoxide; Reproductive toxicity; Embryo development; Mouse. |
| Ano: 2011 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022011000300024 |
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| Bustos-Obregón,Eduardo; Esveile,Christian; Contreras,Julio; Maurer,Inge; Sarabia,Luis. |
| Reduction of O2 delivery to tissues damage them, including the seminiferous epithelium. Recently, population working in high altitude has increased, so that the study of hypobaric hypoxia on spermatogenesis becomes of interest. In this study we used two groups of male, sexually mature mice Control (C) (540 meters above sea level (masl)) and chronic simulated hypobaric hypoxia (CSHH) (4,600 masl) exposed during 8, 16, 24 or 33 days. Hematocrit; reticulocytosis; testicular, epididymal and seminal vesicle weight; seminiferous epithelium height, tubular diameter, sperm count and morphology and testicular parenchyme and spermatozoa membranes lipoperoxidation were measured. Weight of testis, epididymis and seminal vesicle were reduced but they recuperate at 33... |
| Tipo: Journal article |
Palavras-chave: Hypoxia; Lipoperoxidation; Mouse; Testis. |
| Ano: 2006 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022006000400030 |
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| Bustos-Obregón,Eduardo; Poblete,Daniel; Catriao,Roberto; del Sol,Mariano; Fernandes,Fábio Henrique. |
| We evaluated the sperm parameters such as cauda epididymis weight, sperm count, sperm morphology and sperm DNA stability of adult CF-1 male mice treated daily (oral exposure) with the toxic sodium arsenite (As, 7.0 mg/kg/body weight); Melatonin (Me, 10.0 mg/kg/bw), Me (10.0 mg/kg/bw) plus As (7.0 mg/kg/bw) and Negative Control (NaCl 0.9%) to assess acute (8.3 days), chronic (33.2 days) and recovery of testicular damage (66.4 days). Arsenic decreases the number of sperm from chronic treatment (33.2 days) and this effect continued until 66.4 days of treatment. The toxic effect of As also altered the morphology of spermatozoa in all treatment periods when compared to the negative control group. However, Metalonin induced protective effects in periods of 33.2... |
| Tipo: Journal article |
Palavras-chave: Mouse; Melatonin; Arsenic; Sperm; DNA stability. |
| Ano: 2013 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000400017 |
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| Bustos-Obregón,Eduardo; Esponda,Pedro; Sarabia,Luis. |
| The spermatogenesis is regulated by the hypotalamus-hypophysis gonadal axis, and the androgens play a fundamental role in their last stages. The administration of the antiandrogen flutamide interferes with the function of androgen dependent organs (Prostate (P) and Seminal Vesicle (VS)). In this study flutamide (10mg/Kg b.w.) was injected to 10 mice and vehicle to the controls (n=6). The mice were sacrificed at 24 (n=5) and 72 (n=5) hours. In testicular sections the tubular diameter (TD) and height of seminiferous epithelium (EH) were measured. P and VS were macerated, and concentrations of fructose (VS) and zinc (P) were determined. No significant difference exists in the DT among the groups. However, the 72 hrs group showed EH higher than the control (p... |
| Tipo: Journal article |
Palavras-chave: Flutamide; Mouse; Spermatogenesis; Seminal vesicles; Prostate. |
| Ano: 2006 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022006000300008 |
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| Penna-Videau,S; Bustos-Obregón,E; Cermeño-Vivas,J. R; Chirino,D. |
| The restriction of the mechanisms of cell proliferation in murine seminiferous epithelium, in terms of induction of programmed cell death until recently has not been fully analyzed. The aim of this work was to assess the effect of Malathion (MP) on testicular morphology and function in mouse spermatogenesis. For the experiments, male albino mice of strain NMRI-IVIC, weighing between 30-40 g were used, and divided into control and experimental groups of 5 each. The animals of the experimental groups were injected with a single dose of MP: 241mg/kg weight (1/12 LD 50 ) resuspended in 0.9% saline, intraperitoneally. Animals were sacrificed at 8.3, 16.6 and 33.2 days post-injection (first, second and third spermatogenic cycles). Testicular samples were... |
| Tipo: Journal article |
Palavras-chave: Malathion; Testis; Mouse; Apoptosis; Morphology; P53; Proliferation. |
| Ano: 2012 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000400023 |
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| Furuoka, Hidefumi; Yabuzoe, Atushi; Horiuchi, Motohiro; Tagawa, Yuichi; Yokoyama, Takashi; Yamakawa, Yoshio; Shinagawa, Morikazu; Sata, Tetsutaro. |
| Monoclonal antibodies to the prion protein (PrP) have been of critical importance in the neuropathological characterization of PrP-related disease in men and animals. To determine the influence of species-specific amino-acid substitutions recognized by monoclonal antibodies, and to investigate the immunohistochemical reactivity of the latter, analyses were carried out on brain sections of cattle with bovine spongiform encephalopathy, sheep with scrapie, mice infected with scrapie, and human beings with Creutzfeldt-Jakob disease (CJD) or Gerstmann-Sträussler-Sheinker disease (GSS). Immunoreactivity varied between the antibodies, probably as the result of differences in the amino-acid sequence of the prion protein in the various species. Some monoclonal... |
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Palavras-chave: BSE; Cattle; CJD; GSS disease; Man; Mouse; Scrapie; Sheep. |
| Ano: 2007 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1054 |
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| MAEDA, Kanami; LEE, Dong-Soo; YANAGIMOTO UETA, Yoshiko; SUZUKI, Hiroshi; 鈴木, 宏志. |
| Rat uterine sensitization-associated gene-1 (USAG-1) mRNA is expressed in the uterus during the peri-implantation period, and its mRNA expression in uterine epithelial cells is highest on day 5 of pregnancy. On the other hand, since changes in USAG-1 mRNA expression in the mouse uterus are not seen during the estrous cycle, USAG-1 expression might be specifically regulated by embryonic factors rather than by the maternal environment. However, the expression pattern and function of USAG-1 in the mouse uterus have not been determined. Thus, we examined the tissue-specific USAG-1 mRNA expression in the uteri of ICR mice during peri-implantation using real-time quantitative PCR. Uterine tissues, such as the myometrium, luminal epithelium, and stroma, were... |
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Palavras-chave: Implantation; Mouse; MRNA; Uterine sensitization-associated gene-1 (USAG-1); Uterus. |
| Ano: 2007 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3024 |
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