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	Provedor de dados Biol. Res. (1) BJM (1) Autor Amorim,Rosa Valéria da Silva (1) Campos-Takaki,Galba Maria de (1) Fukushima,Kazutaka (1) JABERI,ELHAM (1) SEPEHRIZADEH,ZARGHAM (1) SETAYESH,NED A (1) Souza,Wanderley de (1) YAZDI,MOJTABA TABATABAEI (1) Mais... Palavra-chave Mucor racemosus (2) Chitosan (1) Cunninghamella elegans (1) NADH-cytochrome b s reductase (1) Soluble isoform (1) Submerged culture (1) Mais... Tipo do documento Info:eu-repo/semantics/article (1) Journal article (1) Ano 2009 (1) 2001 (1) País Brazil (1) Chile (1) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Cloning, molecular characterization and expression of a cDNA encoding a functional NADH-cytochrome b5 reductase from Mucor racemosus PTCC 5305 in E. coli Biol. Res. SETAYESH,NED A; SEPEHRIZADEH,ZARGHAM; JABERI,ELHAM; YAZDI,MOJTABA TABATABAEI. The present work aims to study a new NADH-cytochrome b5 reductase (cb5r) from Mucor racemosus PTCC 5305. A cDNA coding for cb s r was isolated from a Mucor racemosus PTCC 5305 cDNA library. The nucleotide sequence of the cDNA including coding and sequences flanking regions was determined. The open reading frame starting from ATG and ending with TAG stop codon encoded 228 amino acids and displayed the closest similarity (73%) with Mortierella alpina cb s r. Lack of hydrophobic residues in the N-terminal sequence was apparent, suggesting that the enzyme is a soluble isoform. The coding sequence was then cloned in the pET16b transcription vector carrying an N-terminal-linked His-Tag® sequence and expressed in Escherichia coli BL21 (DE3). The enzyme was then... Tipo: Journal article Palavras-chave: Mucor racemosus; NADH-cytochrome b s reductase; Soluble isoform. Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000200001 Faster chitosan production by mucoralean strains in submerged culture BJM Amorim,Rosa Valéria da Silva; Souza,Wanderley de; Fukushima,Kazutaka; Campos-Takaki,Galba Maria de. Production of chitosan was conducted using two Mucoralean strains, Mucor racemosus and Cunninghamella elegans. Chitosan was extracted from mycelia of M. racemosus and C. elegans at different growth phases on YPD medium. In both fungi, chitosan was rapidly produced, while highest yield of extractable chitosan was found in 24h of cultivation in submerged culture. The yield of chitosan isolated from dry mycelia of M. racemosus was about 40% higher than from C. elegans. The degree of N-acetylation of chitosan was 49% in M. racemosus and 20% in C. elegans, and the D-glucosamine contents were about 48% and 90%, respectively. Tipo: Info:eu-repo/semantics/article Palavras-chave: Chitosan; Submerged culture; Mucor racemosus; Cunninghamella elegans. Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000100005 Registros recuperados: 2 Primeira ... 1 ... Última

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