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| Bertassoli,Bruno Machado; Costa,Emanuela Silva; Sousa,Cristiane Aparecida; Albergaria,Juliano Douglas Silva; Maltos,Kátia L. Melo; Goes,Alfredo Miranda; Matins,Thais Maria da Mata; Silva,Gerluza Aparecida Borges; Jorge,Erika Cristina. |
| ABSTRACT: Dental pulp stem cells (DPSC) have been showing a considerable potential for regenerative medicine. Pulps were collected from lower incisors (n=2) through direct access of the tooth pulp chamber. The isolated cells were cultured in alfa-MEM 10% FBS, in standard culture conditions. At the third passage, DPSC were characterized by flow cytometry (MHCI, CD54, CD73, CD90, CD45, CD11 and CD34); RT-PCR for Nanog gene; and their differentiation capacity in osteogenic, adipogenic and chondrogenic cell lines. Isolated cells exhibited adhesion capacity to plastic; fusiform morphology, and 80% confluence reached in approximately 3 days. These cells have also revealed positive expression for CD54, CD73 and CD90 markers; and negative expression for CD11, CD34... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mesenchymal stem cells; Dental pulp; Murine; Phenotypic characterization; Cellular differentiation. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100335 |
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| Gonçalves,Paulo Bayard; Graves,Charles. |
| The effect of fetuin, an a1-glycoprotein that has protease inhibitor activity, on the biock to potyspermy was determined. Cumulus-oocyte complexes from eCG primed mice were matured in vitro in the presence of 0, 0.01, 0.1, l and 10mg/ml of fetuin in modifica TCM 199. Both in vivo and in vitro matured oocytes were fertilized in the presence of fetuin and incubated for 6 and 24h. Fetuin present in a concentration of 1 mg/ml in the ferlilization but not in the maturation medium was able to induce polyspermy in 52.4% of the eggs. There was a positive relationship between concentration of fetuin in the fertilization medium and the proportion of polyspermic eggs (p<0.05). A significant interaclion between 0.1 and l mg/ml of fetuin during maturation and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Murine; Block to polyspermy; Fetuin; Supernumerary sperm. |
| Ano: 1997 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84781997000100022 |
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