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| Luz,Viviane Kopp da; Silveira,Solange Ferreira da Silveira; Fonseca,Gabriela Magalhães da; Groli,Eder Licieri; Figueiredo,Ricardo Garcia; Baretta,Diego; Kopp,Mauricio Marini; Magalhães Junior,Ariano Martins de; Maia,Luciano Carlos da; Oliveira,Antonio Costa de. |
| ABSTRACT The increase of yield potential in new rice (Oryza sativa L.) varieties has been a major challenge for genetic improvement. The generation of mutants, followed by their characterization, constitutes a great possibility to isolate and select genes and genotypes that present agronomic traits of interest. This study aimed to evaluate the effect of chemical mutagen ethyl methane sulphonate (EMS) on agronomic traits in 340 M3 families of rice derived from BRS Querência cultivar. Seeds from each family and the original genotype BRS Querência were sown in the experimental field, and the characters main panicle length, main panicle weight, main panicle grain weight, flag leaf width and plant height were evaluated. Data were subjected to analysis of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Oryza sativa; Ethyl methane sulphonate; Mutagenic; Variability. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052016000100041 |
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| Oliveira,R.J.; Mantovani,M.S.; da Silva,A.F.; Pesarini,J.R.; Mauro,M.O.; Ribeiro,L.R.. |
| The compounds 6-dimethylaminopurine and cycloheximide promote the successful production of cloned mammals and have been used in the development of embryos produced by somatic cell nuclear transfer. This study investigated the effects of 6-dimethylaminopurine and cycloheximide in vitro, using the thiazolyl blue tetrazolium bromide colorimetric assay to assess cytotoxicity, the trypan blue exclusion assay to assess cell viability, the comet assay to assess genotoxicity, and the micronucleus test with cytokinesis block to test mutagenicity. In addition, the comet assay and the micronucleus test were also performed on peripheral blood cells of 54 male Swiss mice, 35 g each, to assess the effects of the compounds in vivo. The results indicated that both... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Somatic cell nuclear transfer; 6-Dimethylaminopurine; Cycloheximide; Genotoxic; Mutagenic. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000400287 |
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