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| Registros recuperados: 11 | |
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| Ullmann,Leila Sabrina; Dias,Ramiro das Neves; Cagnini,Didier Quevedo; Yamatogi,Ricardo Seiti; Oliveira,Jose Paes; Nemer,Viviane; Teixeira,Rodrigo Hidalgo Friciello; Biondo,Alexander Welker; Araújo Jr.,João Pessoa. |
| Abstract Background: Mycobacterium is an important zoonotic agent with companion, livestock and wildlife animals reportedly playing a role as reservoirs. Although its association with reptiles has been described, the disease cycle remains to be fully established, particularly in snakes. Accordingly, this study aimed to report the occurrence of mycobacteriosis with clinical pneumonia in one exotic python snake (Python molurus) and one native green snake (Philodryas olfersii) from the Sorocaba Zoo, São Paulo state, Brazil. Methods: Diagnosis was based on necropsy, histopathological examination, Ziehl-Neelsen stain and immunohistochemistry. Results: Using a nested PCR followed by DNA sequencing and bioinformatics analysis, the causative Mycobacterium... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Captive snakes; Mycobacterium; Public health. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992016000100320 |
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| Sircili,Marcelo Palma; Roxo,Eliana; Leão,Sylvia Cardoso. |
| Mycobacterium avium complex (MAC) species cannot be discriminated by the usual methods of biochemical identification of mycobacteria. This study showed that amplification by PCR of DT1 and DT6, two single copy sequences identified in the genome of M. avium serotype 2, the insertion sequence IS1245, found to be consistently present in M. avium strains and the heat-shock protein gene hsp65, followed by restriction polymorphism analysis, are rapid and accurate tests for the differentiation of the species M. avium, M. intracellulare, and M. scrofulaceum. |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Amplification; PCR; MAC; Mycobacterium; Identification. |
| Ano: 1999 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000200011 |
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| PARDO,Renata Bonini; LANGONI,Hélio; MENDONÇA,Lia Jeanne Pereira; CHI,Kung Dahr. |
| This study was performed considering the public health hazards related to the elimination of mycobacteria through milk of dairy cows suspected or positive for tuberculosis presenting no clinical alterations. A total of 780 milk samples from 52 animals, positive or suspected for tuberculosis, according to Stormont's test, were analysed to detect Mycobacterium spp. The samples consisted of 300 ml/cow, collected in the first milking of the day, during 15 days. Frozen samples were sent to the laboratory, inoculated in Löwenstein-Jensen with reduced glicerol (0.5%) and Stonebrink media and kept under 37ºC for at least 90 days. The genus of each observed colony was initially confirmed by Ziehl-Neelsen and auramin staining methods. The isolation of Mycobacterium... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Tuberculosis; Cattle; Milk; Public health; Mycobacterium. |
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962001000600007 |
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| Basílio-de-Oliveira,Carlos Alberto; Eyer-Silva,Walter A.; Valle,Heliomar de Azevedo; Rodrigues,Ana Lúcia; Pimentel,Ana Luisa Pinheiro; Morais-de-Sá,Carlos Alberto. |
| Mycobacterial pseudotumor (MP) is a rare pathologic presentation of both Mycobacterium tuberculosis and non-tuberculous mycobacterial disease, hitherto reported to occur only in immunosuppressed patients with or without human immunodeficiency virus infection. This lesion shares close pathologic resemblance to certain mesenchymal neoplasms, particularly Kaposi's sarcoma (KS), from which it must be properly differentiated due to distinct prognosis and therapy. We report a case of MP obliterating the lumen of the appendix vermiformis in a 34-year-old patient who died of complications of AIDS at our hospital in Rio de Janeiro. A total of 24 cases of MP (including our patient) have been described in the literature. MP has been found especially in lymph nodes,... |
| Tipo: Info:eu-repo/semantics/report |
Palavras-chave: HIV infection; Kaposi's sarcoma; Mycobacterium; Pseudotumor; Spindle cell. |
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702001000200008 |
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| Lilenbaum,W.; Marassi,C.D.; Oelemann,W.M.R.. |
| Paratuberculosis is a chronic enteritis that affects ruminants and is caused by Mycobacterium avium paratuberculosis (Map). The disease is worldwide spread and causes important economic losses. In Brazil, the bacillus was isolated in the south and northeast regions of the country and in Rio de Janeiro, but there are no enough epidemiological studies about its occurrence. Isolation of Map from tissues or fecal samples is 100% specific, but Map shows the most fastidious growth of all mycobacteria. Incubation lasts 8 -12 weeks, with a dependency on exogenous mycobactin J. Diagnostic tests based on specific DNA sequences allow fast and secure identification, and PCR has been used to confirm positive culture results and to identify Map in feces, milk and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Paratuberculosis; Bovine; Mycobacterium; Cattle; Map. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000400001 |
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| Moraes,Paulo Ricardo de Souza; Chimara,Erica; Telles,Maria Alice da Silva; Ueki,Suely Yoko Misuka; Cunha,Eunice Atsuko Totumi; Honer,Michael Robin; Leão,Sylvia Cardoso. |
| Non-tuberculous mycobacteria isolated at the Central Public Health Laboratory from Mato Grosso do Sul in 2003 and 2004 were identified by conventional phenotypic methods (TI) and by PCR-Restriction Enzyme Analysis (PRA) using the hsp65 gene as target (PRA-hsp65). With 15 of the 32 analysed isolates, results of both methods were concordant, being 8 Mycobacterium avium, 3 M. fortutium, 1 M. kansasii, 1 M. flavescens, 1 M. peregrinum and 1 Nocardia brasiliensis. TI of 12 isolates was inconclusive. Novel PRA-hsp65 patterns were observed with 11 isolates. Medical data were evaluated for inference of clinical relevance of these isolates. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mycobacterium; PCR; Identification. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000200013 |
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| Sgarioni,Sônia Aparecida; Hirata,Rosario Dominguez Crespo; Hiroyuki Hirata,Mario; Leite,Clarice Queico Fujimura; Prince,KarinaAndrade de; Leite,Sergio Roberto de Andrade; Vedovello Filho,Dirceu; Siqueira,Vera Lucia Dias; Caleffi-Ferracioli,Katiany Rizzieri; Cardoso,Rosilene Fressatti. |
| Milk is widely consumed in Brazil and can be the vehicle of agent transmission. In this study, was evaluated the occurrence of Mycobacterium bovis and non-tuberculous mycobacteria (NTM) in raw and pasteurized milk consumed in the northwestern region of Paraná, Brazil. Fifty-two milk samples (20 pasteurized and 32 raw) from dairy farms near the municipality of Maringa, Parana State, Brazil were collected. Milk samples were decontaminated using 5% oxalic acid method and cultured on Lowenstein-Jensen and Stonebrink media at 35 °C and 30 °C, with and without 5-10% CO2. Mycobacteria isolates were identified by morphological features, PCR-Restriction Fragment Length Polymorphism Analysis (PCR-PRA) and Mycolic acids analysis. Thirteen (25%) raw and 2 (4%)... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Non-tuberculous mycobacteria; Milk; Mycobacterium; PCR-PRA; Mycolic acids analysis. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200046 |
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| Registros recuperados: 11 | |
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