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| Moura,L.; Dohms,J.; Almeida,J.M.; Ferreira,P.S.; Biffi,C.P.; Backes,R.G.. |
| Adhesion proteins from Mycoplasma gallisepticum (MG) encoded by cytadhesion genes mgc1 and mgc2 were cloned into plasmid vectors and transformed into E. coli. Seventeen groups of specific-pathogen free (SPF), birds at four weeks of age were used to inoculate these two proteins (MGC1 and MGC2) mixed into an oil emulsion creating a novel MG vaccine. Six different protein concentrations (50, 100, 200, 400, 800, and 1000µg/bird) were tested with two equal concentration doses at four and seven weeks of age. In addition, many control groups were needed such as bacterin, membrane, no vaccine or challenge, oil emulsion alone, and no vaccine but challenged. Three weeks following the second vaccination, 50% of the birds in each treatment group were challenged with... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Evaluation; Development; Mycoplasmas; Poultry; Vaccine. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352012000600024 |
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| Santos,Mônica de Oliveira; Castro,Nadya da Silva; Pereira,Maristela; Soares,Célia Maria de Almeida. |
| This is a report on the analysis of genes involved in translation of the complete genomes of Mycoplasma hyopneumoniae strain J and 7448 and Mycoplasma synoviae. In both genomes 31 ORFs encoding large ribosomal subunit proteins and 19 ORFs encoding small ribosomal subunit proteins were found. Ten ribosomal protein gene clusters encoding 42 ribosomal proteins were found in M. synoviae, while 8 clusters encoding 39 ribosomal proteins were found in both M. hyopneumoniae strains. The L33 gene of the M. hyopneumoniae strain 7448 presented two copies in different locations. The genes encoding initiation factors (IF-1, IF-2 and IF-3), elongation factors (EF-G, EF-Tu, EF-Ts and EF-P), and the genes encoding the ribosome recycling factor (frr) and one polypeptide... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mycoplasmas; Mycoplasma synoviae; Mycoplasma hyopneumoniae; Translation; Genome. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000200010 |
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