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Effect of the injection of an extract of Ascaris suum on macrophage activation during the early phase of Mycobacterium bovis BCG infection in C57Bl/6 mice BJMBR
Ferreira,A.P.; Aarestrup,F.M.; Bonecini-Almeida,M.G.; Souza,E.E.; Gomes,E.A.; Corrêa,J.O.A.; Teixeira,H.C..
Injection of an Ascaris suum extract (Asc) affects both the humoral and cellular immune responses to unrelated antigens when it is co-administered with these antigens. In the present study we evaluated the effect of Asc on macrophage activation in the early phase of Mycobacterium bovis BCG (Pasteur strain TMCC 1173) infection in C57Bl/6 mice. C57Bl/6 mice were injected intraperitoneally (ip) with 0.1 mg BCG (BCG group) or BCG plus 1 mg Asc (BCG + Asc group). The peritoneal exudates were obtained at 2, 7 and 14 days after infection. The numbers of IFN-<FONT FACE="Symbol">g</FONT>-secreting cells were assessed by the ELISPOT assay. Nitric oxide (NO) production was measured by the Griess method and by the evaluation of NADPH diaphorase activity in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mycobacterium bovis Ascaris suum macrophage activation TNF-<; FONT FACE=Symbol>; A<; /FONT>; NADPH diaphorase.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001100014
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