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Morphoquantitative Study of Rattus norvegicus Submucosal Plexus by Different Neuronal Evidentiation Histochemical Techniques International Journal of Morphology
Góis,Marcelo Biondaro; Hermes-Uliana,Catchia; Paltanin,Alessandra; Pontes,Willian; Araújo,Eduardo José De Almeida; Miranda Neto,Marcilio Hubner; Sant'Ana,Débora Mello Gonçales.
Enteric nervous plexuses have been the object of several studies, specially the myenteric plexus whose studies describe its organization, functions and alterations. On the other hand, the submucosal plexus has been less studied and still needs descriptive studies. To analyze morphologically and quantitatively submucosal neurons of the jejunum of 90-day-old healthy rats using different techniques for neuronal staining as a way to provide normality data to compare with future experimental studies. Whole mount preparations of the jejunum were submitted to Giemsa, NADH-diaphorase and NADPH-diaphorase techniques to stain the total neuronal population, more metabolically active subpopulation and subpopulation of nitrergic neurons, respectively. Neurons of the...
Tipo: Journal article Palavras-chave: Enteric nervous system; Meissner's plexus; Giemsa; NADH-diaphorase; NADPH-diaphorase.
Ano: 2016 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022016000400050
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Removal of the cortical projections alters expression of NOS in the different cell types of the superficial layers of the superior colliculus in rats Anais da ABC (AABC)
TENÓRIO,FRANK; GIRALDI-GUIMARÃES,ARTHUR; MENDEZ-OTERO,ROSALIA.
Nitric oxide has several biological roles and nitric oxide synthase (NOS) is expressed in the nervous system, and co-localizes with NADPH-diaphorase. The superficial layers of the superior colliculus (SC), which receive retinal and cortical inputs, present NADPH-d staining in a sub-population of neurons that include all cell types. We have previously shown, by NADPH-diaphorase, that eye enucleation alters the intracellular distribution of NOS. Here, we studied the effect of cortical ablation on NOS expression by neurons in collicular superficial layers. Our results show that cortical ablation alters the proportion of different NOS-positive cell types, but not the intracellular distribution of the enzyme.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Superior colliculus; Nitric oxide; Visual cortex; NADPH-diaphorase.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652002000400008
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Nitric oxide synthase activity in tissues of the blowfly Chrysomya megacephala: Fabricius, 1794 Biocell
Faraldo,A. C.; Sá-Nunes,A; Faccioli,L. H.; Del Bel,E. A.; Lello,E.
Although insects lack the adaptive immune response of the mammalians, they manifest effective innate immune responses, which include both cellular and humoral components. Cellular responses are mediated by hemocytes, and humoral responses include the activation of proteolytic cascades that initiate many events, including NO production. In mammals, nitric oxide synthases (NOSs) are also present in the endothelium, the brain, the adrenal glands, and the platelets. Studies on the distribution of NO-producing systems in invertebrates have revealed functional similarities between NOS in this group and vertebrates. We attempted to localize NOS activity in tissues of naïve (UIL), yeast-injected (YIL), and saline-injected (SIL) larvae of the blowfly Chrysomya...
Tipo: Info:eu-repo/semantics/article Palavras-chave: NO synthase; Nitric oxide; NADPH-diaphorase; Chrysomya megacephala; Blowfly; Tissues.
Ano: 2007 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452007000200002
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Axotomy increases NADPH-diaphorase activity in the dorsal root ganglia and lumbar spinal cord of the turtle Trachemys dorbigni BJMBR
Partata,W.A.; Krepsky,A.M.R.; Marques,M.; Achaval,M..
Seven days after transection of the sciatic nerve NADPH-diaphorase activity increased in the small and medium neurons of the dorsal root ganglia of the turtle. However, this increase was observed only in medium neurons for up to 90 days. At this time a bilateral increase of NADPH-diaphorase staining was observed in all areas and neuronal types of the dorsal horn, and in positive motoneurons in the lumbar spinal cord, ipsilateral to the lesion. A similar increase was also demonstrable in spinal glial and endothelial cells. These findings are discussed in relation to the role of nitric oxide in hyperalgesia and neuronal regeneration or degeneration.
Tipo: Info:eu-repo/semantics/article Palavras-chave: NADPH-diaphorase; Axotomy; Turtle.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000400018
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NADPH-diaphorase activity in area 17 of the squirrel monkey visual cortex: neuropil pattern, cell morphology and laminar distribution BJMBR
Franca,J.G.; do-Nascimento,J.L.M.; Picanço-Diniz,C.W.; Quaresma,J.A.S.; Silva,A.L.C..
We studied the distribution of NADPH-diaphorase activity in the visual cortex of normal adult New World monkeys (Saimiri sciureus) using the malic enzyme "indirect" method. NADPH-diaphorase neuropil activity had a heterogeneous distribution. In coronal sections, it had a clear laminar pattern that was coincident with Nissl-stained layers. In tangential sections, we observed blobs in supragranular layers of V1 and stripes throughout the entire V2. We quantified and compared the tangential distribution of NADPH-diaphorase and cytochrome oxidase blobs in adjacent sections of the supragranular layers of V1. Although their spatial distributions were rather similar, the two enzymes did not always overlap. The histochemical reaction also revealed two different...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Nitric oxide synthase; Neocortex; NADPH-diaphorase; Cytochrome oxidase; Primates.
Ano: 1997 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000900008
Registros recuperados: 5
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