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| VARELA,NELSON M; QUIÑONES,LUIS A; ORELLANA,MYRIAM; PONIACHIK,JAIME; CSENDES,ATTILA; SMOK,GLADYS; RODRIGO,RAMÓN; CÁCERES,DANTE D; VIDELA,LUIS A. |
| CYP2E1 enzyme is related to nonalcoholic steatohepatitis (NASH) due to its ability for reactive oxygen species production, which can be influenced by polymorphisms in the gene. The aim of this study was to investigate hepatic levels, activity, and polymorphisms of the CYP2E1 gene to correlate it with clinical and histological features in 48 female obese NASH patients. Subjects were divided into three groups: (i) normal; (ii) steatosis; and (iii) steatohepatitis. CYP2E1 protein level was assayed in microsomes from liver biopsies, and in vivo chlorzoxazone hydroxylation was determined by HPLC. Genomic DNA was isolated for genotype analysis through PCR. The results showed that liver CYP2E1 content was significantly higher in the steatohepatitis (45%; p=0.024)... |
| Tipo: Journal article |
Palavras-chave: Steatohepatitis; NASH; CYP2E1; Obesity; Genotype; Chlorzoxazone. |
| Ano: 2008 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000100010 |
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| Stefano,J.T.; Pereira,I.V.A.; Torres,M.M.; Bida,P.M.; Coelho,A.M.M.; Xerfan,M.P.; Cogliati,B.; Barbeiro,D.F.; Mazo,D.F.C.; Kubrusly,M.S.; D'Albuquerque,L.A.C.; Souza,H.P.; Carrilho,F.J.; Oliveira,C.P.. |
| Liver fibrosis occurring as an outcome of non-alcoholic steatohepatitis (NASH) can precede the development of cirrhosis. We investigated the effects of sorafenib in preventing liver fibrosis in a rodent model of NASH. Adult Sprague-Dawley rats were fed a choline-deficient high-fat diet and exposed to diethylnitrosamine for 6 weeks. The NASH group (n=10) received vehicle and the sorafenib group (n=10) received 2.5 mg·kg-1·day-1 by gavage. A control group (n=4) received only standard diet and vehicle. Following treatment, animals were sacrificed and liver tissue was collected for histologic examination, mRNA isolation, and analysis of mitochondrial function. Genes related to fibrosis (MMP9, TIMP1, TIMP2), oxidative stress (HSP60, HSP90, GST), and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: NASH; Fibrosis; Mitochondrial dysfunction; Sorafenib. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000500408 |
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| Soto,Marianela; González,Lien; Peralta,Esther Lilia; Pérez,R. |
| El viroide del enanismo del lúpulo (HSVd) es el agente causal de la cachexia de los cítricos, enfermedad que puede ocasionar pérdidas importantes en este cultivo. En Cuba se ha informado la presencia del HSVd en cítricos en un alto porcentaje de plantas y se dispone de métodos de detección basados en ensayos biológicos en plantas y su combinación con el análisis de los ácidos nucleicos mediante electroforesis en geles de poliacrilamida e hibridación de ácidos nucleicos. Estos métodos involucran un paso previo de inoculación en un amplificador biológico cítrico, a partir del cual se analizan los ácidos nucleicos, lo cual impone como limitante el requisito de al menos tres meses de incubación. El objetivo del presente trabajo ha sido desarrollar una... |
| Tipo: Journal article |
Palavras-chave: HSVd; Viroide; Detección; NASH; Cítricos. |
| Ano: 2008 |
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1010-27522008000100001 |
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