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| Herman,Braden A.; Ferguson,Kaylee M.; Fernandez,Jared V.B.; Kauffman,Samantha; Spicher,Jason T.; King,Rachel J.; Halterman,Julia A.. |
| Abstract Current diets contain an increasing amount of salt and high fructose corn syrup, but it remains unclear as to how dietary salt and fructose affect organ function at the molecular level. This study aimed to test the hypothesis that consumption of high salt and fructose diets would increase tissue-specific expression of two critical osmotically-regulated genes, nuclear factor of activated T-cells 5 (NFAT5) and aldose reductase (AR). Fifty Sprague-Dawley rats were placed on a control, 4% NaCl, 8% NaCl, or 64% fructose diet for eight weeks. Fourteen different tissue samples were harvested and snap-frozen, followed by RNA purification, cDNA synthesis, and NFAT5 and AR gene expression quantification by real-time PCR.Our findings demonstrate that NFAT5... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: NFAT5; Aldose reductase; Salt; Fructose; Real-time PCR. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000300452 |
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