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| Hipolito,M; Catroxo,M. H. B; Martins,A. M. C. R. P. F; Melo,N.A; Pituco,E. M; Galleti,N.T.C; Ranzani-Paiva,M. J. T; Mouriño,J.L.P; Ferreira,C.M. |
| In this study thirty shrimp samples from commercial marine shrimp (L. vannamei) farms of southern region of Brazil were obtained. Hepatopancreas and shell scrapings fragments collected in these animals were processed by transmission electron microscopy using negative staining (rapid preparation), immunoelectron microscopy and immunocytochemistry (immunolabelling with colloidal gold particles) techniques. On the transmission electron microscopy a great number of white spot virus particles, ovoid or bacilliform-to-ellipsoid, measured 230-290 nm in length and 80-160 nm in diameter with intra-nuclear projections were visualized by the negative staining technique in 27 (90%) out of 30 samples examined. Using immunoelectron microscopy technique, the anti-VP 664... |
| Tipo: Journal article |
Palavras-chave: White spot syndrome virus; Negative staining; Immunoelectron microscopy; Immunocytochemistry; Marine shrimp; Litopenaeus vannamei. |
| Ano: 2012 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000200067 |
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