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	Provedor de dados BJMBR (2) Autor Passaglia,L.M.P. (2) Potrich,D.P. (2) Schrank,I.S. (2) Bressel,T.A. (1) Palavra-chave Nif genes (2) 16S rDNA (1) Azospirillum amazonense (1) Azospirillum brasilense (1) GlnB (1) NifENX operon (1) Promoter activity (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2001 (2) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Partial characterization of nif genes from the bacterium Azospirillum amazonense BJMBR Potrich,D.P.; Passaglia,L.M.P.; Schrank,I.S.. Azospirillum amazonense revealed genomic organization patterns of the nitrogen fixation genes similar to those of the distantly related species A. brasilense. Our work suggests that A. brasilense nifHDK, nifENX, fixABC operons and nifA and glnB genes may be structurally homologous to the counterpart genes of A. amazonense. This is the first analysis revealing homology between A. brasilense nif genes and the A. amazonense genome. Sequence analysis of PCR amplification products revealed similarities between the amino acid sequences of the highly conserved nifD and glnB genes of A. amazonense and related genes of A. brasilense and other bacteria. However, the A. amazonense non-coding regions (the upstream activator sequence region and the region between the... Tipo: Info:eu-repo/semantics/article Palavras-chave: Azospirillum amazonense; Nif genes; GlnB; 16S rDNA. Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000900002 Sequencing and promoter analysis of the nifENXorf3orf5fdxAnifQ operon from Azospirillum brasilense Sp7 BJMBR Potrich,D.P.; Bressel,T.A.; Schrank,I.S.; Passaglia,L.M.P.. A 40-kb DNA region containing the major cluster of nif genes has been isolated from the Azospirillum brasilense Sp7 genome. In this region three nif operons have been identified: nifHDKorf1Y, nifENXorf3orf5fdxAnifQ and orf2nifUSVorf4. The operons containing nifENX and nifUSV genes are separated from the structural nifHDKorf1Y operon by about 5 kb and 10 kb, respectively. The present study shows the sequence analysis of the 6045-bp DNA region containing the nifENX genes. The deduced amino acid sequences from the open reading frames were compared to the nif gene products of other diazotrophic bacteria and indicate the presence of seven ORFs, all reading in the same direction as that of the nifHDKorf1Y operon. Consensus sigma54 and NifA-binding sites are... Tipo: Info:eu-repo/semantics/article Palavras-chave: Azospirillum brasilense; Nif genes; NifENX operon; Promoter activity. Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001100003 Registros recuperados: 2 Primeira ... 1 ... Última

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