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Use of oval conidia as a tool to assess the genetic transfer among Colletotrichum sublineolum mutants BABT
Nunes,Maria Paula; Souza-Paccola,Ednéia Aparecida de; Andrade-Nóbrega,Gisele Maria; Paccola-Meirelles,Luzia Doretto.
The aim of this study was to assess oval conidia production in mutants for the reductase nitrate gene in C. sublineolum and verify the possibility of using them as a tool to transfer the genetic material in this species. The mutants used in the present study lost the ability to form falcate conidia but all produced oval conidia. The number of nuclei by conidium was evaluated. Oval conidia were efficient in the heterokaryon formation and these heterokaryons in liquid culture medium they also produced oval conidia in abundance. Recombinants were obtained and the genetic exchanges were confirmed by the RAPD analyses.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Genetic recombination; Nit mutants; Parameiosis.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000100021
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Vegetative compatibility and heterokaryon formation between different isolates of Colletotrichum lindemuthianum by using the nit mutant system BJM
Carvalho,Camila Rodrigues de; Mendes-Costa,Maria Cristina.
Colletotrichum lindemuthianum, the causative agent of bean anthracnose, is one of the most common pathogens leading to expressive damage to plants beyond presenting noticeable variability. The knowledge on vegetative compatibility groups (VCGs) is of particular interest in asexual fungi as they subdivide the population in groups that can exchange genetic information via heterokaryosis and the parasexual cycle. Among the techniques used in studies about vegetative compatibility groups, the obtainment of nit mutants is apparent. This paper is aimed at obtaining heterokaryons between different isolates of C. lindemuthianum, grouping them in VCGs and evaluating their genetic variability by using the nit mutants system. Nit mutants were obtained from 20 single...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Colletotrichum lindemuthianum; Anthracnose; Heterokaryosis; Nit mutants; VCG.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100044
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