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Experimental and bioinformatic approaches for analyzing and visualizing cyanobacterial nitrogen and hydrogen metabolism Electron. J. Biotechnol.
Wünschiers,Röbbe; Axelsson,Rikard; Vellguth,Martin; Lindblad,Peter.
Many cyanobacteria are capable of utilizing light energy for nitrogen fixation. As a by-product of this nitrogenase mediated catalysis, hydrogen gas is produced. Several approaches to increase hydrogen production from cyanobacteria exist. Usually, these approaches are non-targeted. Here we exemplify how DNA-microarray based gene-expression analysis and bioinformatic visualization techniques can be used to analyze nitrogen and hydrogen metabolism from the filamentous, heterocyst forming cyanobacterium Nostoc PCC 7120. We analyzed the expression of 1249 genes from major metabolic categories under nitrogen fixing and non-nitrogen fixing growth. Of the selected genes, 494 show a more than 2-fold expression difference in the two conditions analyzed. Under...
Tipo: Journal article Palavras-chave: Bioinformatics; Cyanobacteria; DNA-microarrays; Gene expression; Metabolic pathways; Nitrogen availability; Nitrogen fixation.
Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000400008
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Effects of Nitrate and Labile Carbon on Denitrification of Southern Temperate Forest Soils Chilean J. Agric. Res.
Pérez,Cecilia A; Carmona,Martín R.; Fariña,José M.; Armesto,Juan J..
The pressure for anthropogenic land use changes and logging of temperate forests in southern Chile is rapidly increasing, with its potentially high impacts on the capacity of soils to retain important limiting elements. We tested the hypotheses that logging increases the denitrification rates and nitrate and C limitation of denitrifiers activity would be higher in soils of unlogged, old-growth forests than in soils of logged forests. Potential denitrification rates were estimated by the acetylene inhibition assay in intact soil cores in laboratory short-term aerobic incubations using the following treatments: 0.7 mmol NO3-N addition, the same nitrate addition plus 23.3 mmol C-glucose, and controls (no additions) with and without 10% v/v acetylene. Forest...
Tipo: Journal article Palavras-chave: Acetylene inhibition assay; Lowland evergreen forests; Selective logging; Nitrogen availability.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392010000200008
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