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| Portugal,M.E.G.; Souza,E.M.; Pedrosa,F.O.; Benelli,E.M.. |
| Streptococcus mutans is a Gram-positive bacterium present in the oral cavity, and is considered to be one of the leading causes of dental caries. S. mutans has a glnK gene, which codes for a PII-like protein that is possibly involved in the integration of carbon, nitrogen and energy metabolism in several organisms. To characterize the GlnK protein of S. mutans, the glnK gene was amplified by PCR, and cloned into the expression vectors pET29a(+) and pET28b(+). The native GlnK-Sm was purified by anion exchange (Q-Sepharose) and affinity (Hi-Trap Heparin) chromatography. The GlnK-His-Sm protein was purified using a Hi-Trap Chelating-Ni2+ column. The molecular mass of the GlnK-His-Sm proteins was 85 kDa as determined by gel filtration, indicating that this... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: GlnK; Streptococcus mutans; Nitrogen metabolism. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000500003 |
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| Sánchez,E.; Ávila-Quezada,G.; Gardea,A.A.; Muñoz,E.; Ruiz,J.M.; Romero,L.. |
| The objective of this work was to determine the effect of different P doses on nitrogen assimilation in roots and leaves of green beans plants (Phaseolus vulgaris L. cv. Strike). Phosphorus was applied in the nutrient solution as H3PO4, at the nutrient doses of: P1 = 0.40 mM; P2 = 0.80 mM; P3 = 1.60 mM; P4 = 3.20 mM; P5 = 4.80 mM, and P6 = 6.40 mM P. Our results indicate that both P toxicity and deficiency gave similar responses to N assimilation. Phosphorus and NO3 - interacted on the absorption and translocation processes affecting N assimilation. The deficiency (P1), and toxicity (P6) treatments, diminished root absorption of NO3- in 15% and 36%, respectively, respect to the optimum dose (P3), thus reducing nutrient availability for assimilation. This... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Phaseolus vulgaris L.; Nitrogen metabolism; Green bean; Phosphorus; Deficiency; Toxicity. |
| Ano: 2009 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572009000100003 |
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| Gandra,Jefferson Rodrigues; Takiya,Caio Seiti; Oliveira,Euclides Reuter de; Paiva,Pablo Gomes de; Goes,Rafael Henrique de Tonissi e Buschinelli de; Gandra,Érika Rosendo de Sena; Araki,Hayne Mayumi Cariolano. |
| ABSTRACT This study was undertaken to determine the effects of chitosan and whole raw soybean on nutrient intake and total tract digestion, nitrogen utilization, microbial protein synthesis, blood metabolites, and energy balance of dairy heifers. Twelve Jersey heifers (6±0.5 months of age and 139.50±25.56 kg of live weight; mean ± standard deviation) were randomly assigned to a replicated Latin square design with a 2 × 2 factorial arrangement. The experimental period consisted of 14 days of adaptation to diets, six days of sampling, and five days of washout. The experimental diets were: control (CO); chitosan (CHI, inclusion of 2.0 g kg−1 DM of chitosan); whole raw soybean (WS, 163.0 g kg−1 of WS on diet DM basis); and chitosan + whole raw soybean... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Antimicrobial; Nitrogen metabolism; Oilseed; Rumen modulator. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982016000300130 |
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| Zhao,Yumei; Tian,Gang; Chen,Daiwen; Zheng,Ping; Yu,Jie; He,Jun; Mao,Xiangbing; Yu,Bing. |
| ABSTRACT This experiment was conducted to evaluate the effects of dietary protein and net energy (NE) levels on growth performance, nutrient digestibility, nitrogen metabolism, and faecal microbiota of growing-finishing pigs. Eighteen crossed barrows were randomly allocated into one of three dietary treatments: high protein + high NE diet, low protein + high NE diet, and low protein + low NE diet. The whole experiment lasted 90 days and was divided into three phases (phase I: 25-50 kg; phase II: 50-75 kg; phase III: 75-105 kg). All pigs were individually housed in a metabolism cage and subjected to four-day total faeces and urine collection period at the end of each phase. There was no significant difference in growth performance, nutrient digestibility,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Faecal microbiota; Nitrogen metabolism; Nutrient digestibility; Pigs. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982019000100516 |
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| Nardi,Serenella; Pizzeghello,Diego; Schiavon,Michela; Ertani,Andrea. |
| ABSTRACT In recent years, the use of biostimulants in sustainable agriculture has been growing. Biostimulants can be obtained from different organic materials and include humic substances (HS), complex organic materials, beneficial chemical elements, peptides and amino acids, inorganic salts, seaweed extracts, chitin and chitosan derivatives, antitranspirants, amino acids and other N-containing substances. The application of biostimulants to plants leads to higher content of nutrients in their tissue and positive metabolic changes. For these reasons, the development of new biostimulants has become a focus of scientific interest. Among their different functions, biostimulants influence plant growth and nitrogen metabolism, especially because of their... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Hormones; Nitrogen metabolism; Carbon metabolism; Phenylpropanoid pathway; Stress. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162016000100018 |
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