|
|
|
|
|
Martinelli,José A.; Bocchese,Carla A.C.; Xie,Weiping; O'Donnell,Kerry; Kistler,Harold C.. |
Surveys of soybean (Glycine max) seed grown in South Brazil revealed infection with Fusarium graminearum. To determine if members of this complex were pathogenic to soybean, six strains derived from soybean were added to soil at a rate of 10³ macroconidia/ ml or individual pods were inoculated with 10(4) macroconidia/ml. Seedlings grown in infested soil developed small necrotic lesions in the crown and upper roots. Pods inoculated with conidia developed large (>1 cm), dark brown, necrotic lesions. Younger pods inoculated with the fungus blighted and dropped from the plant. Strains of the F. graminearum complex recovered from lesions on the crown, roots and pods of soybean plants were identified as lineage 1, 2 or 8 by obtaining the DNA sequence from the... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Glycine max; Gibberella zeae; Pathogenicity; Deoxinivalenol; Nivalenol; Compatibility groups. |
Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582004000500004 |
| |
|
| |
|
|
Venkataramana,M.; Shilpa,P.; Balakrishna,K.; Murali,H.S.; Batra,H.V.. |
Hundred Fusarium culmorum strains, isolated from freshly harvested maize grain samples from Southern parts of India, were incubated in czapek-dox medium and analyzed for trichothecene (DON/NIV) production. The mPCR assay was standardized targeting trichothecene metabolic pathway genes viz., Tri6, Tri7, Tri13 for detection of trichothecene (DON/NIV) chemotypes and rDNA gene for specific detection of F. culmorum species. Primers for targeted genes were designed and used to predict whether these isolates could produce deoxynivalenol/nivalenol, 94 isolates were able to produce DON/NIV by mPCR assay. Chemical analysis of DON/NIV was carried out for mPCR positive isolates by high performance-thin layer chromatography (HPTLC). To check the practical usefulness of... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Deoxynivalenol; Nivalenol; Multiplex PCR assay; HPTLC. |
Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200009 |
| |
|
|
|