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Novel Antifungal Compounds, Spermine-Like and Short Cyclic Polylactates, Produced by Lactobacillus harbinensis K.V9.3.1Np in Yogurt ArchiMer
Mosbah, Amor; Delavenne, Emilie; Souissi, Yasmine; Mahjoubi, Mouna; Jehan, Philippe; Le Yondre, Nicolas; Cherif, Ameur; Bondon, Arnaud; Mounier, Jerome; Baudy-floc'H, Michele; Le Blay, Gwenaelle.
Lactobacillus harbinensis K.V9.3.1Np was described as endowed with high antifungal activity. Most of the studies associated this activity to the produced organic acids, i.e., lactic acid, acetic acid, and hexanoic acid. The aim of this study was to purify and identify, other not yet described, antifungal molecules produced by L. harbinensis K.V9.3.1 Np when used in yogurt fermentation. Active compounds were extracted through several extraction processes using organic solvents and protein precipitation. The fractions of interest were purified using flash chromatography and preparative HPLC for specific characterization. The bioactive compounds identification was performed using Nuclear Magnetic Resonance and Mass Spectrometry. Activity tests against...
Tipo: Text Palavras-chave: Spermine; Polylactates; Lactobacillus; Extraction; Purification; Ms; Nmr.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00616/72773/72221.pdf
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