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| Pinheiro, Marcos Vinícius Marques; Universidade Federal de Viçosa; Martins, Fabrina Bolzan; Universidade Federal de Itajubá; Cruz, Ana Claudia Ferreira da; Universidade Federal de Viçosa; Carvalho, Ana Cristina Portugal Pinto de; Embrapa Agroindústria Tropical; Oliveira, Evelyn Jardim de; Universidade Federal de Viçosa; Otoni, Wagner Campos; Universidade Federal de Viçosa. |
| This study establishes a protocol for the induction of somatic embryogenesis in Anthurium andraeanum cv. Eidibel. The experiment was arranged in a completely randomized 5 x 5 x 5 factorial design using five explant types (whole leaves, half leaves; petiole; nodal segments and root segments) from in vitro plantlets; five auxins: indole-3-acetic acid (IAA), α-naphthaleneacetic acid (NAA), indole-3-butyric acid (IBA), 2,4-dichlorophenoxyacetic acid (2,4-D), and 4-amino-3,5,6-trichloropicolinic acid (Picloram); at five concentrations (0, 2.5, 5, 7.5 and 10 µM), with five replications using five Petri dishes. The cultures were maintained in a growth room at 25 ± 2ºC in the dark. The explant type was investigated for the induction of somatic embryogenesis in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: 5.00.00.00-4 auxins; Nodal segments; Somatic embryos Ciências Agrárias. |
| Ano: 2014 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/16557 |
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| Sá,Aline de Jesus; Lédo,Ana da Silva; Lédo,Carlos Alberto da Silva; Pasqual,Moacir; Silva,Ana Veruska Cruz da; Silva Junior,Josué Francisco da. |
| In micropropagation, especially for mangaba tree botanical variety of Northeastern Brazil, limiting aspects such as ethylene accumulation in the cultivation flask and loss of vigor in subcultures have been observed. This study was aimed at assessing the technical and scientific knowledge of the in vitro propagation of botanical mangaba tree variety and at improving the micropropagation protocol, establishing the in vitro cultivation time, the best type of flask sealing and explant at different micropropagation stages. For the establishment phase and for the first and second subcultures, the MS medium with 3% sucrose and 0.6% agar, supplemented with 1 mg L-1 IAA and 1 mg L-1 BA was used. Evaluations were performed at 30, 50 and 65 days of in vitro... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Hancornia speciosa; Tissue culture; In vitro ethylene; Nodal segments. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542012000400004 |
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