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| Breuil, Gilles. |
| The sea bass, Dicentrarchus labrax, has been subjected to intensive aquaculture on the Mediterranean cost for ten years. Marine fish hatcheries are key factors in the aquaculture field, and face different pathologies, especially one of viral origin (nodavirus) that could challenge their feasibility. Consequently, we have tried to provide some data on larvae and spawning stocks. Firstly, we have studied the immunoglobulins, components of the specific humoral immunity, and their role as antibodies. On adult basses, these immunoglobulins are tetrameric molecules with high molecular weight (850 kD), made of heavy (H) - 80 kD and light (L) - 26 kD chains. In the eggs, simplest forms such as monomeric or dimeric are brought to the fore; they are located under... |
| Tipo: Text |
Palavras-chave: Egg; Cytometry; Immunity system; Larvae; Immunoglobulin; Nodavirus; Dicentrarchus labrax; Hatchery; Ontogenese; Cytometrie; Immunoglobuline; Nodavirus; Oeuf; Larve; Nurserie; Dicentrarchus labrax. |
| Ano: 1997 |
URL: http://archimer.ifremer.fr/doc/1997/these-1265.pdf |
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| Breuil, Gilles; Mouchel, Olivier; Fauvel, Christian; Pepin, Jean-francois. |
| Reproduction of nodavirus disease was performed by experimental infection of sea bass eggs during fertilization or at larval stage 4 with 2 genetically distinguishable nodavirus strains (Sb1 and Sb2) isolated from sea bass collected along the Atlantic and Mediterranean French coast. The pathogenicity of the virus strains was assigned after detection of the virus by ELISA and immunohistochemistry (IHC). The Atlantic (Sb1) strain was more pathogenic than the Mediterranean (Sb2) strain during the fertilization step whilst both strains were pathogenic following experimental exposure of 4 d old larvae. Virus lesions developed in the brain 4 to 6 d following experimental exposure. Experimental ELISA proved very sensitive for detecting the nodavirus in Sb1 or Sb2... |
| Tipo: Text |
Palavras-chave: ELISA; Nodavirus; Fish encephalopathy. |
| Ano: 2001 |
URL: http://archimer.ifremer.fr/doc/2001/publication-814.pdf |
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| Coeurdacier, Jean-luc; Dutto, Gilbert; Gasset, Eric; Blancheton, Jean-paul. |
| Reared sea bass were submitted to six stressful situations: hyperoxia with or without hypercapnia, increased stocking density in an open flow or recirculating system, transfer to another tank and nodavirus injection. The potentially negative impact of these factors on the lives of sea bass was investigated by measuring 9 water parameters and 19 fish parameters including total serum protein (TSP). TSP has already been used to evaluate stress. The present study investigates data of previous and new experiments, concentrating on the potential use of TSP as a routine indicator to assess welfare in sea bass reared on fish farms. In the current experiment, oxygen and carbon dioxide were seen to affect levels of TSP, but alterations were too erratic to enable... |
| Tipo: Text |
Palavras-chave: Fish stress; Fish welfare; Protein; Serum; Fish transfer; Nodavirus; Sea bass. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00043/15382/12740.pdf |
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