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	Provedor de dados BJMBR (1) BJB (1) Autor COSTA,M. J. (1) Denapolis,P.M.A. (1) GUERRA,C. D. R. (1) Han,S.W. (1) KALININ,A. L. (1) Moraes,J.Z. (1) RANTIN,F. T. (1) SEVERI,W. (1) Sacramento,C.B. (1) Mais... Palavra-chave Hypoxia (2) Normoxia (2) Buccal and opercular volumes (1) Hypoxia-responsive element (1) Ischemic diseases (1) Piaractus mesopotamicus (1) SV40-DTS (1) Transfection efficiency (1) Ventilatory parameters (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2010 (1) 2000 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Ventilatory flow relative to intrabuccal and intraopercular volumes in the serrasalmid fish Piaractus mesopotamicus during normoxia and exposed to graded hypoxia BJB KALININ,A. L.; SEVERI,W.; GUERRA,C. D. R.; COSTA,M. J.; RANTIN,F. T.. Ventilation volume (<img src="http:/img/fbpe/rbbio/v60n2/a08vg.gif" alt="a08vg.gif (265 bytes)" align="absmiddle"> – mlH2O.min–1 ), respiratory frequency (fR – breaths.min–1) and tidal volume (V T – mlH2O.breath–1 ) were measured in a group of Piaractus mesopotamicus (650.4 ± 204.7 g; n = 10) during normoxia and in response to graded hypoxia. The fR was maintained constant, around 100 breaths.min–1, from normoxia until the O2 tension of the inspired water (PiO2) of 53 mmHg, below which it increased progressively, reaching maximum values (157.6 ± 6.3 breaths.min–1) at 10 mmHg. The V T rose from 1.8 ± 0.1 to 6.0 ± 0.5 and 5.7 ± 0.4 mlH2O. breath–1 in the PiO2 of 16 and 10 mmHg, respectively. The <img src="http:/img/fbpe/rbbio/v60n2/a08vg.gif"... Tipo: Info:eu-repo/semantics/article Palavras-chave: Hypoxia; Normoxia; Ventilatory parameters; Buccal and opercular volumes; Piaractus mesopotamicus. Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0034-71082000000200008 Gene expression promoted by the SV40 DNA targeting sequence and the hypoxia-responsive element under normoxia and hypoxia BJMBR Sacramento,C.B.; Moraes,J.Z.; Denapolis,P.M.A.; Han,S.W.. The main objective of the present study was to find suitable DNA-targeting sequences (DTS) for the construction of plasmid vectors to be used to treat ischemic diseases. The well-known Simian virus 40 nuclear DTS (SV40-DTS) and hypoxia-responsive element (HRE) sequences were used to construct plasmid vectors to express the human vascular endothelial growth factor gene (hVEGF). The rate of plasmid nuclear transport and consequent gene expression under normoxia (20% O2) and hypoxia (less than 5% O2) were determined. Plasmids containing the SV40-DTS or HRE sequences were constructed and used to transfect the A293T cell line (a human embryonic kidney cell line) in vitro and mouse skeletal muscle cells in vivo. Plasmid transport to the nucleus was monitored by... Tipo: Info:eu-repo/semantics/article Palavras-chave: SV40-DTS; Hypoxia-responsive element; Transfection efficiency; Normoxia; Hypoxia; Ischemic diseases. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000800005 Registros recuperados: 2 Primeira ... 1 ... Última

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