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Detection of toxins A/B and isolation of Clostridium difficile and Clostridium perfringens from dogs in Minas Gerais, Brazil BJM
Silva,Rodrigo Otávio Silveira; Santos,Renata Lara Resende; Pires,Prhiscylla Sadanã; Pereira,Luiz Carlos; Pereira,Silvia Trindade; Duarte,Marina Carvalho; Assis,Ronnie Antunes de; Lobato,Francisco Carlos Faria.
The objective of this study was to detect C. difficileA/B toxins and to isolate strains of C. perfringensand C. difficile from diarrheic and non-diarrheic dogs in Brazil. Stool samples were collected from 57 dogs, 35 of which were apparently healthy, and 22 of which were diarrheic. C. difficileA/B toxins were detected by ELISA, and C. perfringensand C. difficilewere identified by multiplex PCR. C. difficileA/B toxins were detected in 21 samples (36.8%). Of these, 16 (76.2%) were from diarrheic dogs, and five (23.8%) were from non-diarrheic dogs. Twelve C. difficile strains (21.1%) were isolated, of which ten were A+B+and two were A-B-. All non-toxigenic strains were isolated from non-diarrheic animals. The binary toxin gene cdtBwas found in one strain,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Nosocomial diarrhea; Small animals; Colitis; Enteritis; Canine.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000100019
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First confirmed case of Clostridium difficile-associated diarrhea in foals in Brazil Ciência Rural
Silva,Rodrigo Otávio Silveira; Moreira,Felipe Moraghi; Rezende,Jackeline Viana; Pires,Prhiscylla Sadanã; Maranhão,Renata de Pino Albuquerque; Palhares,Maristela Silveira; Lobato,Francisco Carlos Faria.
Despite of the substantial role of Clostridium difficile in causing diarrhea and colitis in foals, there have been no confirmed diagnoses of disease caused by this bacteria in Brazil. In this paper, we describe confirmed cases of colitis caused by C. difficile in two foals in Brazil. Two five-month-old foals with a five-day history of diarrhea after antibiotic treatment for a respiratory disease were treated at the Veterinary Hospital of the Universidade Federal de Minas Gerais. C. difficile A/B toxins were detected, and toxigenic strains of C. difficile were isolated from the foals' feces. The treatment was based on fluid therapy and antibiotics (metronidazole and ceftiofur), and the animals experienced a gradual recovery. The association between the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Colitis; Equine; Nosocomial diarrhea.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782012000300018
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Antimicrobial susceptibility of Clostridium difficile isolated from animals and humans in Brazil Ciência Rural
Silva,Rodrigo Otávio Silveira; Oliveira Junior,Carlos Augusto; Diniz,Amanda Nádia; Alves,Guilherme Guerra; Guedes,Roberto Maurício Carvalho; Vilela,Eduardo Garcia; Lobato,Francisco Carlos Faria.
The objective of this study was to evaluate antimicrobial susceptibility in Clostridium difficile strains isolated from animals and humans in Brazil. The 54 C. difficile strains used were isolated from stool samples from piglets (n=16), dogs (n=13), humans (n=13), foals (n=8) calves (n=2), an ocelot (n=1) and a maned wolf (n=1). Antimicrobial susceptibility was determined using the serial plate agar dilution method for penicillin, florfenicol, oxytetracycline, erythromycin, vancomycin, metronidazole and tylosin. The C. difficile strains assessed were susceptible to metronidazole and vancomycin. Florfenicol resistance was rarely observed; 52 (96.4%) strains were sensitive to this antimicrobial. Five (9.3%), five (9.3%), 14 (25.9%) and 20 (37.0%) strains...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Nosocomial diarrhea; Pseudomembranous colitis; Resistance.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782014000500013
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