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| Colihueque,Nelson; Corrales,Olga; Parraguez,Margarita. |
| Cytogenetic analysis of Trichomycterus areolatus, collected from the Tijeral and Huilma Rivers in southern Chile has shown a diploid chromosome number of 2n = 54, a fundamental number of FN = 106, and a karyotypic formula of 44m + 8sm + 2st. Intra-individual polymorphism of chromosome number (2n = 54, 55 and 56) in specimens from the Huilma River has also been documented, providing further evidence of the occurrence of this phenomenon in Trichomycterus. The karyotype exhibited large chromosome pairs: metacentric pairs 1 (relative length 7.54%), 2 (5.75%) and 3 (5.09%), submetacentric pair 23 (5.25%), and subtelocentic pair 27 (5.28%). Nuclear DNA content analysis showed an average value of 5.04 ± 1.09 pg/nucleus. This DNA content is higher than the mean... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Karyotype; Nuclear DNA content; Trichomycterus; Siluriformes. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200014 |
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| GALLARDO-ESCÁRATE,CRISTIAN; ÁLVAREZ-BORREGO,JOSUÉ; VON BRAND,ELISABETH; DUPRÉ,ENRIQUE; DEL RÍO-PORTILLA,MIGUEL ÁNGEL. |
| In observations by confocal or conventional fluorescence microscopy, important factors should be considered in order to obtain accurate images. One of them, such as the fluorescence bleaching from highest intensity to lowest signal of fluorescence is a common problem with several DNA fluorochromes and especially for DAPI stain. The fluorescence of DAPI fades rapidly when it is exposed to UV light, under optimal conditions of observation. Although the fading process can be retarded using a mounting medium with antifading reagents, the photochemical process underlying the fluorescence decay has not yet been fully explained. In addition, no relationship between fluorescence fading and nuclear DNA content has been tested. In order to test this relationship, we... |
| Tipo: Journal article |
Palavras-chave: Nuclear DNA content; Fluorescence fading; Image analysis; DAPI. |
| Ano: 2007 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000100004 |
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| Timbó,Ana Luiza de Oliveira; Pereira,Roselaine Cristina; Santos,Vanderley Borges dos; Souza Sobrinho,Fausto; Davide,Lisete Chamma. |
| Flow cytometry allows to estimate the DNA content of a large number of plants quickly. However, inadequate protocols can compromise the reliability of these estimates leading to variations in the values of DNA content the same species. The objective of this study was to propose an efficient protocol to estimate the DNA content of Brachiaria spp. genotypes with different ploidy levels using flow cytometry. We evaluated four genotypes (B. ruziziensis diploid and artificially tetraploidized; a tetraploid B. brizantha and a natural triploid hybrid), three buffer solutions (MgSO4, Galbraith and Tris-HCl) and three species as internal reference standards (Raphanus sativus, Solanum lycopersicum e Pisum sativum). The variables measured were: histogram score (1-5),... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Brachiaria ruziziensis; Brachiaria brizantha; Colchicine; Nuclear DNA content. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542012000600001 |
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| Couto,Evellyn Giselly de Oliveira; Davide,Livia Maria Chamma; Bustamante,Fernanda de Oliveira; Pinho,Renzo Garcia Von; Silva,Tallyta Nayara. |
| The development of homozygous breeding lines in maize may be accelerated through the use of haploids. Thus, the obtaining and prior identification of haploids generated by the haploid inducer lines is an important factor. The purpose of this study was to identify haploids by flow cytometry and to correlate the nuclear DNA content to the morphological and morphometric traits of the seeds that gave rise to them. In addition, molecular markers were used to confirm the androgenetic nature of the haploid. The seeds obtained were derived from the cross between the inbred line W23 and the commercial hybrid P30F90. Among these seeds, a group was selected, putative haploids, whose embryo was white and the pericarp purplish. This group, consisting of 330 seeds, was... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Zea mays; Nuclear DNA content; Androgenetic inheritance; R-navajo; Microsatellites. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542013000100003 |
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