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	Provedor de dados BABT (1) Rev. Bras. Frutic. (1) Autor Alves,Paulo César Martins (1) Amaral,Paulo de Paiva Rosa (1) Bouktila,Dhia (1) Capdeville,Guy de (1) Cherif,Mejda (1) Gharsallah,Charfeddine (1) Habachi-Houimli,Yosra (1) Makni,Mohamed (1) Martins,Natália Florêncio (1) Silva,Felipe Rodrigues da (1) Souza Júnior,Manoel Teixeira (1) Sébéi,Abdennour (1) Mais... Palavra-chave Nucleotide-binding site (NBS) (2) C. papaya (1) Caricaceae (1) Hordeum vulgare (1) Leucine-rich repeat (LRR) (1) Papaya (1) QRT-PCR (1) Spot form net blotch (SFNB) (1) V. cauliflora (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2019 (1) 2006 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Identification and characterization of a resistance gene analog (RGA) from the Caricaceae Dumort family Rev. Bras. Frutic. Amaral,Paulo de Paiva Rosa; Alves,Paulo César Martins; Martins,Natália Florêncio; Silva,Felipe Rodrigues da; Capdeville,Guy de; Souza Júnior,Manoel Teixeira. The majority of cloned resistance (R) genes characterized so far contain a nucleotide-binding site (NBS) and a leucine-rich repeat (LRR) domain, where highly conserved motifs are found. Resistance genes analogs (RGAs) are genetic markers obtained by a PCR-based strategy using degenerated oligonucleotide primers drawn from these highly conserved "motifs". This strategy has the advantage of the high degree of structural and amino acid sequence conservation that is observed in R genes. The objective of the present study was to search for RGAs in Carica papaya L. and Vasconcellea cauliflora Jacq. A. DC. Out of three combinations of primers tested, only one resulted in amplification. The amplified product was cloned in pCR2.1TOPO and than sequenced using M13... Tipo: Info:eu-repo/semantics/article Palavras-chave: Caricaceae; Nucleotide-binding site (NBS); V. cauliflora; C. papaya; Papaya. Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452006000300026 Expression Analysis of Pyrenophora teres f. maculata-Responsive Loci in Hordeum vulgare BABT Habachi-Houimli,Yosra; Cherif,Mejda; Gharsallah,Charfeddine; Sébéi,Abdennour; Makni,Mohamed; Bouktila,Dhia. Abstract Pyrenophora teres f. maculata is the causal agent of barley spot form net blotch (SFNB), a major stubble-borne disease in many barley-growing areas worldwide. In plants, the Nucleotide-Binding Site-Leucine-Rich Repeat (NBS-LRR) gene family functions in immunity against a variety of pathogens and pests. From a pre-established set of NBS-type resistance gene candidates, we have selected three candidate genes, HvNBS10, HvNBS72 and HvNBS85, to analyze their possible involvement in P. teres f. maculata resistance. The studied genes were mapped on chromosomes 5H and 7H. Expression profiles using qRT-PCR, 48 hours after infection by P. teres. f. maculata, revealed that the transcription of all genes acted in the same direction (down-regulation) in both... Tipo: Info:eu-repo/semantics/article Palavras-chave: Spot form net blotch (SFNB); Nucleotide-binding site (NBS); Leucine-rich repeat (LRR); QRT-PCR; Hordeum vulgare. Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100425 Registros recuperados: 2 Primeira ... 1 ... Última

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