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Carraschi, Silvia Patrícia; Garlich, Nathália; Souza-Pollo, Andressa; Pereira, Daniela Isabel Brayer; Cruz, Claudinei da; Ranzani-Paiva, Maria José Tavares. |
Successful disease treatment depends on molecular studies under indoor conditions with experimental infection protocols that facilitate understanding the disease and the drug`s efficacy. The internal transcribed spacer (ITS) region was sequenced from three isolates, which were identified as Saprolegnia aenigmatica. Subsequently, healthy fish were immunosuppressed with dexamethasone (1.2 mg kg-1) and descaled to the skin using a sharp scalpel. These individuals were isolated in individual aquariums maintained at 22°C. Individuals in one group were subcutaneously inoculated with 9,000 zoospores (DDZ treatment), a second group was exposed to oomycetes in water with three colonized baits (DDB), a third group was maintained in water without zoospores (DD), and... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: 5.06.03.00-0 oomycete; Zoosporangium; Oogonium; Saprolegniasis. Aquicultura. |
Ano: 2018 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/38186 |
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