|
|
|
|
|
| Grijalba,Pablo E; Zapata,Raul L; Palmucci,Hemilse E; Baron,Claudio. |
| En raíces de plantas adultas de tomate se observó una lesión castaña de aspecto húmedo, que se extendía 2-4 cm por encima del suelo. Las plantas atacadas se marchitaron y murieron. El objetivo del trabajo fue aislar e identificar al organismo causal de esta sintomatología. Se usaron medios de cultivos generales y selectivos. A las 24 horas desarrollaron colonias con micelio blanco algodonoso. Se evaluó la morfología de la colonia y la producción, morfología y dimensiones de estructuras reproductivas. El rDNA del micelio de un aislamiento fue extraído de cultivos puros con 7-10 días de crecimiento en medios agarizados. La región ITS se amplificó usando primers ITS4 e ITS5, se secuenció y comparó en banco de genes del servidor BLAST- NCBI para verificar su... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Oomycete; Pythium; Tomate. |
| Ano: 2015 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-23722015000100002 |
| |
|
| |
|
|
| Bala, K.; Robideau, G.P.; Désaulniers, N.; Cock, A.W.A.M. de; Lévesque, C.A.. |
| Three new species of Pythium, namely, P. oopapillum, P. emineosum and P. camurandrum are presented in this paper based on morphological descriptions and molecular phylogenetic characterisation. These new species were isolated from various ecological regions in Canada. They have unique morphological features in the genus Pythium, and form distinct clades in maximum parsimony analyses, which are also supported by maximum likelihood phylogeny using general time reversible model (GTR), and Bayesian inference (BI) phylogeny using Markov Chain Monte Carlo (MCMC) analysis methods. A comparative study of the new species with closely related taxa, their clade positions, and morphological features are described in this paper. |
| Tipo: Article / Letter to the editor |
Palavras-chave: COI; ITS rDNA; LSU rDNA; Oomycete; Peronosporales; Pythium; Taxonomy. |
| Ano: 2010 |
URL: http://www.repository.naturalis.nl/record/531810 |
| |
|
|
| COSTA,JAQUELINE FIGUEREDO DE OLIVEIRA; ASSUNÇÃO,IRAÍLDES PEREIRA; LIMA,GAUS SILVESTRE DE ANDRADE; MUNIZ,MARIA DE FÁTIMA SILVA; LUZ,EDNA DORA MARTINS NEWMAN. |
| ABSTRACT In 2013, soursop trees showing symptoms of root rot were observed in a field in Maceió, state of Alagoas, Brazil. It was isolated Phytophthora sp. which pathogenicity was confirmed in the host seedlings. Morphological and physiological characteristics in carrot-agar modified medium were consistent with Phytophthora nicotianae description. The PCR sequences products obtained with ITS1/ITS4 primers were compared to sequences of ribosomal DNA of Phytophthora species from the GenBank database observing high identity with other P. nicotianae isolates. A phylogenetic tree was performed to compare the isolate with other sequences of P. nicotianae, which clustering has been verified with 99% of bootstrap, confirming the morphophysiological studies. This... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Annona muricata; Phytophthora wilt; Oomycete; Etiology. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452016000300405 |
| |
|
|
|
