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| Saponji,Svetlana; Kneevi-Jugovi,Zorica D; Bezbradica,Dejan I; Zuza,Milena G; Saied,Omar Ali; Boskovi-Vragolovi,Nevenka; Mijin,Dusan Z. |
| Lipase from Candida rugosa was covalently immobilized on Sepabeads EC-EP for application for amyl caprylate synthesis in an organic solvent system. Several solvents were tested in terms of biocatalyst stability and the best result was obtained with isooctane. The lipase-catalyzed esterification in the selected system was performed in batch and fluidized bed reactor systems. The influence of several important reaction parameters including temperature, initial water content, enzyme loading, acid/alcohol molar ratio, and time of addition of molecular sieves is carefully analyzed by means of an experimental design. Almost complete conversion (> 99%) of the substrate to ester could be performed in a batch reactor system, using lipase loading as low as 37 mg... |
| Tipo: Journal article |
Palavras-chave: Bioreactors; Covalent immobilization; Ester; Non-aqueous system; Optimization technique. |
| Ano: 2010 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000600012 |
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