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Isolement et caracterisation d'une proteine membranaire ovocytaire capable de fixer specifiquement la vitellogenine ArchiMer
Jugan, P; Vanherp, F.
After solubilization of ovocyte-membranes from the crayfish Orconectes limosus , a component with a molecular weight of 30 kDa and capable to fix vitellogenin specifically, could be characterized by SDS-PAGE and by electroblotting. Because this isolated component is sensible to the digestion of proteolytic enzymes such as pronase, it is assumed that it is a protein. A polyclonal antiserum is produced against this component and its tissue-specificity is verified by ELISA.
Tipo: Text Palavras-chave: Orconectes limosus; Malacostraca; Oogenesis; Biochemical analysis; Cell membranes.
Ano: 1987 URL: http://archimer.ifremer.fr/doc/1987/acte-1382.pdf
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Quelques aspects de la regulation du systeme neuroendocrine produisant la CHH et de la relation entre le rythme circadien et la glycemie ArchiMer
Kallen, J.
In crayfish, the hemolymph glucose level shows an endogenous circadian rhythmicity which is expressed by a nocturnal glucose rise. This study shows that injections of serotonin and dopamine result in hyperglycemia and that injections of met-enkephalin decrease hemolymph glucose level as well as the nocturnal circadian glucose peak. Using immunocytochemistry, these neurotransmitters have been located in the eyestalk and their relationship with the CHH cell system is described.
Tipo: Text Palavras-chave: Orconectes limosus; Astacus leptodactylus; Malacostraca; Cytochemistry; Hormones; Neurotransmitters; Circadian rhythms; Glucose.
Ano: 1987 URL: http://archimer.ifremer.fr/doc/1987/acte-1383.pdf
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