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	Provedor de dados Anais da ABC (AABC) (2) BJM (2) BJPS (1) Electron. J. Biotechnol. (1) Autor AQUINO,TATIANE DE (1) Aragón,Diana Marcela (1) Barberis,Sonia (1) ELLWANGER,JOEL H. (1) Ghori,M. I (1) Grazinoli-Garrido,Rodrigo (1) Hameed,A (1) Iqbal,M. J (1) Mahajan,Vineet (1) Marchese,José (1) Martínez,Fleming (1) PRÁ,DANIEL (1) Prakash,S. (1) Priolo,Nora (1) Quiroga,Evelina (1) RIEGER,ALEXANDRE (1) Rosas,Jaiver Eduardo (1) Shouche,Yogesh S. (1) Sola-Penna,Mauro (1) Sreeramulu,K. (1) Mais... Palavra-chave Organic solvents (6) Alcohol (1) Araujia hortorum Fourn (1) Bacillus sp (1) Chromohalobacter sp. TVSP101 (1) DNA damage (1) Enzyme (1) Halothermophilic protease (1) Ibuprofen (1) Kinetic study (1) Lipases (1) Micronucleus (1) Occupational health (1) Osmolytes (1) P-Nitrophenyl laurate (1) Plant protease (1) Purification (1) Solubility (1) Solution thermodynamics (1) Substrate preferences (1) Mais... Tipo do documento Info:eu-repo/semantics/article (5) Journal article (1) Ano 2016 (1) 2011 (1) 2010 (1) 2009 (1) 2006 (1) 2004 (1) Mais... País Brazil (5) Chile (1) Idioma Inglês (6)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 6 Primeira ... 1 ... Última Inactivation of yeast inorganic pyrophosphatase by organic solvents Anais da ABC (AABC) Grazinoli-Garrido,Rodrigo; Sola-Penna,Mauro. A number of application for enzymes in organic solvents have been developed in chemical processing, food related conversions and analyses. The only unsolved problem related to nonaqueous enzymology is the notion that enzymes in organic solvent are mostly far less active than in water. Therefore, studies concerning the mechanisms by which enzymes are inactivated by organic solvents would reveal a clear understanding of the structure-function relationship of this phenomenon. Here we analyzed the effects of a series of alcohols (methanol, ethanol, 1-propanol and 2-propanol) and acetone on the activity of yeast inorganic pyrophosphatase. We observed that solvents inactivated the enzyme in a dose-dependent manner. This inactivation is also dependent on the... Tipo: Info:eu-repo/semantics/article Palavras-chave: Organic solvents; Enzyme; Yeast inorganic pyrophosphatase; Alcohol. Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652004000400006 DNA damage and cytotoxicity in pathology laboratory technicians exposed to organic solvents Anais da ABC (AABC) AQUINO,TATIANE DE; ZENKNER,FERNANDA F.; ELLWANGER,JOEL H.; PRÁ,DANIEL; RIEGER,ALEXANDRE. The aim of this study was to evaluate potential DNA damage and cytotoxicity in pathology laboratory technicians exposed to organic solvents, mainly xylene. Peripheral blood and buccal cells samples were collected from 18 technicians occupationally exposed to organic solvents and 11 non-exposed individuals. The technicians were sampled at two moments: Monday and Friday. DNA damage and cytotoxicity were evaluated using the Comet Assay and the Buccal Micronucleus Cytome assay. Fifteen subjects (83.5%) of the exposed group to solvents complained about some symptom probably related to contact with vapours of organic solvents. DNA damage in the exposed group to solvents was nearly 2-fold higher on Friday than on Monday, and in both moments the individuals of... Tipo: Info:eu-repo/semantics/article Palavras-chave: DNA damage; Micronucleus; Toxicology; Occupational health; Organic solvents; Xylene. Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000100227 Purification and characterization of an extreme halothermophilic protease from a halophilic bacterium Chromohalobacter sp. TVSP101 BJM Vidyasagar,Malashetty; Prakash,S.; Mahajan,Vineet; Shouche,Yogesh S.; Sreeramulu,K.. An extreme halophilic bacterium was isolated from solar saltern samples and identified based on biochemical tests and 16S r RNA sequencing as Chromohalobacter sp. strain TVSP101. The halophilic protease was purified using ultrafiltration, ethanol precipitation, hydrophobic interaction column chromatography and gel permeation chromatography to 180 fold with 22% yield. The molecular mass of the protease determined by SDS PAGE was 66 kDa. The purified enzyme was salt dependent for its activity and stability with an optimum of 4.5 M NaCl. The optimum temperature for maximum protease activity was 75°C. The protease was optimally active at pH 8 and retained more than 80% of its activity in the range of pH 7-10. Sucrose and glycine at 10% (w/v) were the most... Tipo: Info:eu-repo/semantics/article Palavras-chave: Chromohalobacter sp. TVSP101; Halothermophilic protease; Purification; Organic solvents; Osmolytes. Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000100002 Characterization of a novel lipase from Bacillus sp. isolated from tannery wastes BJM Ghori,M. I; Iqbal,M. J; Hameed,A. Kinetics of a lipase isolated from Bacillus sp. was studied. The enzyme showed maximum activity at pH 9 and temperature 60ºC. The Michaelis constant (K M 0.31 µM) obtained from three different plots i.e., Lineweaver-Burk, Hanes-Wolf and Hofstee, was found to be lower than already reported lipases that confirmed higher affinity of the enzyme for its substrate p-NPL (p-nitrophenyl laurate). Vmax of the enzyme was found to be 7.6 µM/mL/min. Energy of activation calculated from Arrhenius plot was found to be 20.607 kJmol-1. Activation enthalpy (ΔH*) had negative trend and the value for the hydrolysis of p-NPL by the enzyme at optimum temperature was -2.748 kJmol-1 . Activation entropy (ΔS*) and free energy of activation (ΔG*) of the enzyme were found to be... Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacillus sp; Kinetic study; Lipases; P-Nitrophenyl laurate; Tannery wastes; Organic solvents. Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100003 Thermodynamic study of the solubility of ibuprofen in acetone and dichloromethane BJPS Aragón,Diana Marcela; Rosas,Jaiver Eduardo; Martínez,Fleming. Thermodynamic functions, Gibbs energy, enthalpy and entropy for the solution processes of ibuprofen (IBP) in acetone and dichloromethane (DCM) were calculated from solubility values obtained at temperatures ranging from 293.15 K to 313.15 K. The respective thermodynamic functions for mixing and solvation processes as well as the activity coefficients for the solute were calculated. IBP solubility was high and proved similar in both solvents but was greater in DCM than acetone. In addition, the thermodynamic quantities for the transfer process of this drug from cyclohexane to the organic solvents were also calculated in order to estimate the contributions of hydrogen-bonds or of other dipolar interactions. The results were discussed in terms of... Tipo: Info:eu-repo/semantics/article Palavras-chave: Ibuprofen; Solubility; Transfer; Solution thermodynamics; Organic solvents. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000200009 Behavior of Araujiain, a new cysteine phytoprotease, in organic media with low water content Electron. J. Biotechnol. Quiroga,Evelina; Priolo,Nora; Marchese,José; Barberis,Sonia. In this paper we studied the effect of different organic solvents (1-octanol, trichloroethylene, ethanol, ethyl acetate, tetrahydrofuran, cyclohexane, propanone, acetonitrile, dichloromethane, chlorobenzene, N,N-dimethylformamide, acetophenone, diethyl ether, methanol, ethylene glycol and toluene) with low and constant water content on substrate preferences, thermostability and stability (caseinolytic activity retention after 4 h) of proteases of Araujia hortorum Fourn. (Asclepiadaceae). The stability of araujiain was high in N,N-dimethylformamide and ethanol at 40ºC, but decreased at higher temperature. Araujiain substrates preferences in buffer Tris-HCl (pH 8), ethylene glycol and N,N-dimethylformamide exhibited different patterns, but the... Tipo: Journal article Palavras-chave: Araujia hortorum Fourn; Organic solvents; Plant protease; Substrate preferences; Thermostability. Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000100004 Registros recuperados: 6 Primeira ... 1 ... Última

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