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Sperm motility in European hake, Merluccius merluccius, and characterization of its spermatozoa concentration and volume, spermatocrit, osmolality and pH ArchiMer
Groison, Anne-laure; Suquet, Marc; Cosson, Jacky; Mayer, Ian; Severe, Armelle; Bouquet, Jean-marie; Geffen, Audrey J.; Utne-palm, Anne Christine; Kjesbu, Olav Sigurd.
Due to stock declines there is a growing interest in farming of European hake Merluccius merluccius. Thus, knowledge of its sperm biology is of importance not only for purposes of broodstock management, but also for the development of sperm preservation techniques. Hake sperm were collected from mature males caught during the summer-early autumn waters off western Norway and during the winter-early spring in the Bay of Biscay (France). Sperm quality characteristics were assessed after storage at 4 degrees C for 25 +/- 14 h. Average (+/- SD) values for Norwegian and French samples respectively were (i) sperm volume: 3.9 +/- 4.0 and 2.6 +/- 4.0 ml; (ii) spermatozoa concentration: 6.6 +/- 3.2 and 13.9 +/- 5.1 x 10(9) spermatozoa/ml; (iii) spermatocrit: 80.2...
Tipo: Text Palavras-chave: Cryopreservation; Merluccius merluccius; Osmolality; PH; Sperm motility; Spermatocrit.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00003/11393/8021.pdf
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Marine fish spermatozoa: racing ephemeral swimmers ArchiMer
Cosson, J; Groison, A; Suquet, Marc; Fauvel, Christian; Dreanno, Catherine; Billard, R.
After along period of spermatogenesis (several weeks to months), marine fish spermatozoa are delivered at male spawning in seawater (SW) at the same time as ova. In some fish species, as the ova micropyle closes quickly after release, these minute unicells, the spermatozoa, have to accomplish their task of reaching the micropyle within a very brief period (several seconds to minutes), for delivery of the haploid male genetic information to the ova. To achieve this goal, their high-performance motile equipment, the flagellum, must fully activate immediately on contact with the SW and then propel the sperm cell at an unusually high initial velocity. The cost of such 'hyperactivity' is a very rapid consumption of intracellular ATP that outstrips the supply....
Tipo: Text Palavras-chave: Cell activation; Osmolality; Ions; Cell energetics; Motility; Spermatozoon; Marine fish.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-6967.pdf
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