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Modulation of in vitro Osteoclast Activity Mediated by Different Doses of Parathyroid Hormone International Journal of Morphology
Ginani,Fernanda; Mota-Filho,Haroldo Gurgel; Vasconcelos Barreto,Mardem Portela e; Barboza,Carlos Augusto Galvão.
Bone remodeling is a process regulated by the interaction between cells and various molecules such as parathyroid hormone (PTH). The aim of the study was to evaluate the effect of different doses of PTH on osteoclast activity in a culture model of bone organs. Six-day-old male C57BL/6 mice (n=14) were euthanized and the calvariae were dissected and sectioned in the middle, keeping the periosteal and endosteal. The bone fragments were divided into three groups: Group I (control - without adding PTH), Group II (addition of 3 nM PTH) and Group III (30 nM PTH), all cultured in aMEM for up to 72 h osteoclast activity was evaluated by biochemical quantification of calcium released in the culture medium at intervals of 24, 48, and 72 h and by histomorphometric...
Tipo: Journal article Palavras-chave: Parathyroid hormone; Osteoclast; Bone remodeling; Organ culture techniques.
Ano: 2017 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022017000200033
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Tartrate-resistant acid phosphatase as a biomarker of bone turnover in dog Arq. Bras. Med. Vet. Zootec.
Sousa,C.P; Nery,F; Azevedo,J.T; Viegas,C.A; Gomes,M.E; Dias,I.R.
Values of serum tartrate-resistant acid phosphatase ( TRAP) activity were obtained in adult dogs and its biological variability was assessed. Nine healthy skeletally mature Portuguese Podengo dogs were used for the determination of TRAP, total and bone alkaline phosphatase serum activities, and also to study their relationship with serum minerals, namely calcium (Ca), phosphorous (P), and magnesium (Mg). The serum TRAP activity was 2.19±0.56IU/mL, with intra-individual variation of 18.3% and inter-individual variation of 25.6%. Significant correlations were observed between serum TRAP activity and Ca (r=-0.3431; P<0.05), Ca and Mg (r=-0.787; P<0.01), and TRAP and Mg (r=0.397; P<0.05). The results indicate that serum TRAP activity in dog could be...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Dog; Bone metabolism; Bone resorption; Osteoclast.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352011000100007
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