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Bone resorptive activity of osteoclast-like cells generated in vitro by PEG-induced macrophage fusion Biol. Res.
Murillo,Andrea; Guerrero,Carlos A; Acosta,Orlando; Cardozo,Carmen A.
Normal bone remodeling is maintained by a balance between osteoclast and osteoblast activity, whereas defects in osteoclast activity affecting such balance result in metabolic bone disease. Macrophage-macrophage fusion leading to multinucleated osteoclasts being formed is still not well understood. Here we present PEG-induced fusion of macrophages from both U937/A and J774 cell lines and the induced differentiation and activation of osteoclast-like cells according to the expression of osteoclast markers such as tartrate resistant acid phosphatase (TRAP) and bone resorptive activity. PEG-induced macrophage fusion, during the non-confuent stage, signifcantly increased the osteoclastogenic activity of macrophages from cell lines compared to that of...
Tipo: Journal article Palavras-chave: Bone resorption; Macrophage fusion; Osteoclast activity; Osteoclast differentiation; PEG; RANKL.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000200009
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Flavonoids isolated from Tridax procumbens (TPF) inhibit osteoclasts differentiation and bone resorption Biol. Res.
Mamun,Md. Abdullah Al; Islam,Kamrul; Alam,Md. Jahangir; Khatun,Amina; Masihul Alam,M; Al-Bari,Md. Abdul Alim; Alam,Md. Jahangir.
BACKGROUND: The Tridax procumbens flavonoids (TPF), are well known for their medicinal properties among local natives. The TPF are traditionally used for dropsy, anaemia, arthritis, gout, asthma, ulcer, piles, and urinary problems. It also used in treating gastric problems, body pain, and rheumatic pains of joints. The TPF have been reported to increase osteogenic functioning in mesenchymal stem cells. However, their effects on osteoclastogenesis remain unclear. The TPF isolated from T. procumbens and investigated the effects of the TPF inhibit on osteoclast differentiation and bone resorption activities using primary osteoclastic cells. Osteoclast formation was assessed by counting the number of tartrate resistant acid phosphatase (TRAP) positive...
Tipo: Journal article Palavras-chave: Bone resorption; Osteoclast differentiation; Pit formation; TRAP positive cells.
Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100051
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