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| Santerre, Christelle; Sourdaine, Pascal; Marc, Nicolas; Mingant, Christian; Robert, Rene; Martinez, Anne-sophie. |
| The sex-determining system of Crassostrea gigas is still poorly known, especially regarding the potential influence of temperature. In order to address this question, mRNA expressions of actors of the molecular cascade (Cg-DMI, Cg-SoxE, Cg-beta-catenin, Cg-Foxl2/Cg-FoxI2os) and of Oyvlg, a germ cell marker, were investigated by real-time PCR in spat grown at different temperatures (18, 22, 25 and 28 degrees C). In parallel, gonadic differentiation, gametogenesis and sex ratios were assessed by histology at each of these temperatures. Whatever the temperature, Cg-DMI, Cg-SoxE, Cg-beta-catenin and Oyvlg expressions peaked at the same developmental stage, always after Cg-Foxl2/Cg-Foxl2os (around 40-44 dpf for spat grown at 18 degrees C). Temperatures... |
| Tipo: Text |
Palavras-chave: Oyster spat; Temperature; Sex ratio; TSD; Sex determination pathway. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00136/24767/22874.pdf |
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| Vignier, Julien; Rolton, Anne; Soudant, Philippe; Chu, Fu-lin E.; Robert, Rene; Volety, Aswani K.. |
| The 2010 explosion of the Deepwater Horizon (DWH) oil rig generated the largest marine oil spill in US history with millions of barrels of crude oil released in the Gulf of Mexico (GoM). The eastern oyster, Crassostrea virginica, is an ecologically and economically important species in the northern GoM. Due to its biological characteristics (sessile, filter feeding), juvenile oysters may have been affected. This study investigated the effects of surface-collected DWH oil prepared as high-energy water-accommodated fraction (HEWAF) on the survival of 2-month-old oyster spat, and evaluated the potential impacts of HEWAF on particle clearance rate and spat tissue. Exposure of oysters to a range of oil/HEWAF (0-7-66-147-908-3450 mu g tPAH50 (sum of 50... |
| Tipo: Text |
Palavras-chave: Oyster spat; Deepwater Horizon oil; Sublethal; Tissue; Inflammatory response; Histopathology. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00425/53615/54548.pdf |
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| Normand, Julien; Blin, Jean-louis; Jouaux, Aude. |
| Early detection of Pacific oyster spat infected with ostreid herpesvirus 1 (OsHV-1) could prevent introduction of OsHV-1-infected individuals into farming areas or onshore rearing facilities, thus reducing the risk of infection of naïve oysters in such production systems. Experiments were conducted on several hundred oyster spat provided by producers in order to examine whether early rearing practices could be considered as potential risk factors for (1) OsHV-1 infection as detected by molecular methods and (2) spat mortality experimentally induced through thermal challenge. Spat groups collected on oyster beds and hatchery spat reared in growout areas during summer exhibited higher viral DNA contamination and mortalities during the trial than spat kept in... |
| Tipo: Text |
Palavras-chave: Oyster spat; Epidemiology; OsHV-1; QPCR; Thermal challenge. |
| Ano: 2014 |
URL: https://archimer.ifremer.fr/doc/00203/31407/29797.pdf |
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| Lagarde, Franck; Roque D'Orbcastel, Emmanuelle; Ubertini, Martin; Mortreux, Serge; Bernard, Ismael; Fiandrino, Annie; Chiantella, Claude; Bec, Beatrice; Roques, Cecile; Bonnet, Delphine; Miron, Gilles; Richard, Marion; Pouvreau, Stephane; Lett, Christophe. |
| In the context of increasing demand for environmental recovery, aquatic systems may face the challenge of evolving under oligotrophication. This is the case in Mediterranean lagoons, in particular the shellfish-farmed Thau lagoon in France, where we studied recruitment of the Pacific oyster Crassostrea gigas. Oyster spat and environmental parameters were monitored at several sampling sites for 3 yr (2012 to 2014) using an original method with a temporal overlap deployment of collectors to study pre- and post-settlement processes and to identify the best conditions for recruitment. Contrary to the ‘no Pacific oyster reproduction’ paradigm in Mediterranean lagoons, our study showed that recruitment of this introduced species is possible in the Thau lagoon at... |
| Tipo: Text |
Palavras-chave: Crassostrea gigas; Oyster spat; Pediveliger; Metamorphosis; Recruitment; Oligo trophication; Larval ecology. |
| Ano: 2017 |
URL: https://archimer.ifremer.fr/doc/00398/50899/51672.pdf |
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