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| Silva,Rodrigo Martins da; Souto,Eliezer Rodrigues de; Pedroso,Júlio Cézar; Arakava,Ricardo; Almeida,Álvaro Manuel Rodrigues; Barboza,Antônio Augusto Lazarini; Vida,João Batista. |
| During an inspection in plastic houses in Sapopema, Paraná, 90% of tomato plants showed leaf abnormalities, probably associated with herbicide toxity. However, virus like symptoms developed in selected hosts after mechanical inoculatation. RT-PCR reactions using primers for an internal region within the movement protein gene of TMV and ToMV resulted in the amplification of a 409 bp cDNA fragment only by TMV primers. Deduced amino acids showed 100% identity when compared to TMV movement protein and 94% with ToMV. The RT-PCR protocol was efficient for quick and conclusive determination of virus species. The virus was purified and a polyclonal antiserum was raised for future surveys in tomato crops of Paraná. The partial genomic sequence obtained for... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: TMV; P30 protein; Plasticulture. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000500005 |
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