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| Registros recuperados: 67 | |
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| Cruz,A.C.F.G.; Madruga,C.R.M.; Mallmann,C.A.; Moreira,E.L.T.; Botura,M.B.B.; Silva,G.D.S.; Batatinha,M.J.M.. |
| Identificou-se o efeito das aflatoxinas (AFs) sobre o gene p53 de frangos de corte, de linhagem comercial, separados em: grupo experimental, tratado (GT) com ração comercial contendo 2,8ppm de AFs totais durante 21 dias consecutivos, e grupo-controle (GC), sem exposição às AFs. Macroscopicamente, as alterações caracterizaram-se por hepatomegalia e aspecto pálido-amarelado com alguns focos hemorrágicos e, histologicamente, por desarranjo trabecular, pleomorfismo hepatocítico com cariomegalia, degeneração vacuolar intracitoplasmática, necrose com infiltração linfocítica e hiperplasia de ductos biliares. A PCR com os primers GSPT53c-1 com base no gene candidato a p53 (GenBank XM_424937.2) gerou um produto de aproximadamente 350 pares de base. O amplicon... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Frango de corte; Aflatoxinas; Gene p53; PCR-RFLP. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352012000600036 |
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| Machado,M.A.; Nascimento,C.S.; Martinez,M.L.; Silva,M.V.G.B.; Campos,A.L.; Teodoro,R.L.; Verneque,R.S.; Guimarães,S.E.F.. |
| A associação entre os alelos do loco BoLA-DRB3.2, identificados pela técnica de PCR-RFLP, e a produção de leite na raça Gir foi estudada por meio da análise de dados moleculares e fenotípicos de 424 vacas Gir, utilizando um modelo misto, sob modelo animal. Os dados moleculares consistiam dos genótipos dos animais para os alelos do loco BoLA-DRB3.2 e os dados fenotípicos eram referentes à produção de leite em até 305 dias de lactação. O loco é altamente polimórfico nesta raça, sendo identificados sete alelos (BoLA-DRB3.2*4, *8, *11, *19, *28, *41 e *48) que não haviam sido encontrados em animais zebuínos. Dois alelos (*16 e *29) estavam significativamente associados com maiores produções de leite, sugerindo que o próprio loco BoLA-DRB3.2 ou um QTL a ele... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: BoLA-DRB3; PCR-RFLP; Gene candidato; Bovinos; Gir. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352005000300017 |
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| Golfetto,L.; Alves,E.V.; Martins,T.R.; Sincero,T.C.M.; Castro,J.B.S.; Dannebrock,C.; Oliveira,J.G.; Levi,J.E.; Onofre,A.S.C.; Bazzo,M.L.. |
| Persistent human papillomavirus (HPV) infection is an essential factor of cervical cancer. This study evaluated the analytical performance of restriction fragment length polymorphism polymerase chain reaction (PCR-RFLP) assay compared to PapilloCheck® microarray to identify human papilloma virus (HPV) in cervical cells. Three hundred and twenty-five women were analyzed. One sample was used for conventional cytology and another sample was collected using BD SurePath™ kit for HPV tests. Eighty samples (24.6%) were positive for HPV gene by PCR-Multiplex and were then submitted to PCR-RFLP and PapilloCheck® microarray. There was a genotyping agreement in 71.25% (57/80) on at least one HPV type between PCR-RFLP and PapilloCheck® microarray. In 22 samples... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cervical cancer; HPV; Screening; PCR-RFLP; PapilloCheck® microarray. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000500619 |
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| Gibotti,Adriana; Tanaka,Tânia L.; Oliveira,Valéria R.; Taddei,Carla R.; Martinez,Marina B.. |
| In this study, polymorphism in ipa genes was found in five out of nine EIEC serotypes studied. When SalI and HindII were used in RFLP-PCR assays many EIEC serotypes showed polymorphism in ipaB and ipaD. On the other hand, no polymorphism was observed in ipaA and ipaC in these strains. The polymorphism present in EIEC strains is serotype-dependent, since restriction patterns were conserved amongst strains belonging to the same serotype. When IpaB deduced amino acid sequences of S. flexneri M90T and FBC124-13 were compared, ten amino acids changes could be observed mainly in the amino-terminal region. The deduced EIEC IpaD amino-acid sequence presented 91% similarity with the Shigella strain. In this case, amino acid changes were spread out through the whole... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ipa; Enteroinvasive Escherichia coli; Polymorphism; PCR-RFLP. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000100012 |
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| Liang,Hongxia; Liu,Yao; Zhu,Jun; Guan,Peng; Li,Shuangcheng; Wang,Shiquan; Zheng,Aiping; Liu,Huainian; Li,Ping. |
| Sichuan basin, situated in the west of China, is the fourth biggest basin in China. In order to describe a systematic study of the cry2-type genes resources from Bacillus thuringiensis strains of Sichuan basin, a total of 791 Bacillus thuringiensis strains have been screened from 2650 soil samples in different ecological regions. The method of PCR-restriction fragment length polymorphism (PCR-RFLP) was used to identify the type of cry2 genes. The results showed that 322 Bacillus thuringiensis strains harbored cry2-type genes and four different RFLP patterns were found. The combination of cry2Aa/cry2Ab genes was the most frequent (90.4%), followed by cry2Aa (6.8%) and cry2Ab alone (2.5%), and only one novel type of cry2 gene was cloned from one isolate... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bacillus thuringiensis; PCR-RFLP; SDS-PAGE; Novel cry2-type gene. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100018 |
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| Zarrinfar,Hossein; Kaboli,Saeed; Dolatabadi,Somayeh; Mohammadi,Rasoul. |
| Abstract Candida species, especially C. albicans, are commensals on human mucosal surfaces, but are increasingly becoming one of the important invasive pathogens as seen by a rise in its prevalence in immunocompromised patients and in antibiotic consumption. Thus, an accurate identification of Candida species in patients with pulmonary symptoms can provide important information for effective treatment. A total of 75 clinical isolates of Candida species were obtained from the bronchoalveolar lavage fluid of both immunocompromised and immunocompetent patients with pulmonary symptoms. Candida cultures were identified based on nuclear ribosomal Internal Transcribed Spacer (ITS1-ITS2 rDNA) sequence analysis by polymerase chain reaction–restriction fragment... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Candida; Pulmonary; PCR-RFLP. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000100172 |
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| Scarcelli,E.; Piatti,R.M.; Harakava,R.; Miyashiro,S.; Campos,F.R.; Souza,M.C.A.; Cardoso,M.V.; Teixeira,S.R.; Genovez,M.E.. |
| The objectives of the present study were the subtyping of Campylobacter jejuni subsp. jejuni strains obtained from humans and different animal species using PCR-RFLP, and the detection, by means of the same technique, of strains related to serotype PEN O19:LIO 7, the main C. jejuni serotype linked to Guillain-Barré Syndrome (GBS). Seventy C. jejuni strains isolated from human feces (n=33), primates (n=15), dogs (n=5), swine (n=2), bovines (n=1), abortion material from goats (n=2) and poultry carcasses (n=12), all collected in the state of São Paulo, were subtyped by means of PCR-RFLP of fla A gene, using restriction endonucleases Hae III, Afa I and Mbo I. Seven subtypes were observed when using the enzyme Hae III; eight when using Mbo I; and seven when... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Campylobacter jejuni; PCR-RFLP; Subtyping; Guillain-Barré Syndrome. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000400029 |
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| Ozbey,Gokben; Dogan,Yasar; Demiroren,Kaan; Ozercan,Ibrahim Hanifi. |
| The objectives of the present study were to determine Helicobacter pylori via culture, polymerase chain reaction and histopathological diagnosis in 101 children ranging in age from 4 to 18 years, to identify the association among restriction fragment length polymorphism types and clinical disease and to investigate the relationships among different isolates of H. pylori in different age groups. We observed a high prevalence of H. pylori infections in children between the ages of 13 and 18 (75.8%), while children aged 4 to 6 years had the lowest prevalence of infection (40%). H. pylori was detected in 30.7% (31 of 101), 66.3% (67 of 101) and 63.2% (60 of 95) of children as determined by culture methods, PCR and histological examination, respectively. H.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Children; Culture; Helicobacter pylori; PCR-RFLP. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200505 |
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| Becerra V,Viviana; Paredes C,Mario; Debouck,Daniel. |
| The Chilean common bean (Phaseolus vulgaris L.) belongs to the cultivated race Chile and its origin is presumably Andean. The objective of this study was to identify the origin of a group of Chilean accessions based on their genetic relationship with wild material from the Mesoamerican and Andean common bean gene pool. To achieve this objective, universal primers of chloroplast DNA (cpDNA) and mitochondrial DNA (mtDNA) were used to detect polymorphism using Polymerase Chain Reaction - Restriction Fragment Length Polymorphism (PCR-RFLP). Thirty-two genotypes were analyzed, including wild material from Mexico, Ecuador, Peru, Bolivia, and Argentina, as well as Chilean cultivated genotypes belonging to endemic Chilean accession types (Tórtola, Coscorrón, and... |
| Tipo: Journal article |
Palavras-chave: Chilean common bean germplasm; Chloroplast DNA; Mitochondrial DNA; PCR-RFLP. |
| Ano: 2011 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392011000100001 |
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| Dolores Ramos Eva. |
| Con el objeto de estandarizar y perfeccionar una técnica de extracción y purificación de ADN a partir de sangre, semen y pelo de porcinos, se obtuvieron muestras de 35 sementales de línea materna Yorkshire y Landrace, en los cuales se valoró la frecuencia de los genes ESR y PRLR mediante PCR-RFLP. La extracción de ADN a partir de sangre y semen únicamente se estandarizaron. El pelo de sementales fue la mejor opción para obtener una alta cantidad y calidad de ADN. Se identificó la frecuencia de dos genes relacionados con prolificidad: Receptor de Prolactina (PRLR) y Receptor de Estrógeno (ESR); en su amplificación se utilizó PCR-RFLP´s y se identificaron los genotipos. En esta identificación se utilizó la enzima Alu I para PRLR y Pvu II para... |
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Palavras-chave: ADN; Pelo; Sangre; Semen; Cerdos; PCR-RFLP hair; Blood and semen DNA; Boars; PCR-RFLP. |
| Ano: 2012 |
URL: http://hdl.handle.net/10521/888 |
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| Dolores Ramos Eva. |
| Con el objeto de estandarizar y perfeccionar una técnica de extracción y purificación de ADN a partir de sangre, semen y pelo de porcinos, se obtuvieron muestras de 35 sementales de línea materna Yorkshire y Landrace, en los cuales se valoró la frecuencia de los genes ESR y PRLR mediante PCR-RFLP. La extracción de ADN a partir de sangre y semen únicamente se estandarizaron. El pelo de sementales fue la mejor opción para obtener una alta cantidad y calidad de ADN. Se identificó la frecuencia de dos genes relacionados con prolificidad: Receptor de Prolactina (PRLR) y Receptor de Estrógeno (ESR); en su amplificación se utilizó PCR-RFLP´s y se identificaron los genotipos. En esta identificación se utilizó la enzima Alu I para PRLR y Pvu II para... |
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Palavras-chave: ADN; Pelo; Sangre; Semen; Cerdos; PCR-RFLP hair; Blood and semen DNA; Boars; PCR-RFLP. |
| Ano: 2012 |
URL: http://hdl.handle.net/10521/911 |
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| Dolores Ramos Eva. |
| Con el objeto de estandarizar y perfeccionar una técnica de extracción y purificación de ADN a partir de sangre, semen y pelo de porcinos, se obtuvieron muestras de 35 sementales de línea materna Yorkshire y Landrace, en los cuales se valoró la frecuencia de los genes ESR y PRLR mediante PCR-RFLP. La extracción de ADN a partir de sangre y semen únicamente se estandarizaron. El pelo de sementales fue la mejor opción para obtener una alta cantidad y calidad de ADN. Se identificó la frecuencia de dos genes relacionados con prolificidad: Receptor de Prolactina (PRLR) y Receptor de Estrógeno (ESR); en su amplificación se utilizó PCR-RFLP´s y se identificaron los genotipos. En esta identificación se utilizó la enzima Alu I para PRLR y Pvu II para... |
| Tipo: Tesis |
Palavras-chave: ADN; Pelo; Sangre; Semen; Cerdos; PCR-RFLP; Hair; Blood and semen DNA; Boars. |
| Ano: 2007 |
URL: http://hdl.handle.net/10521/1202 |
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| Benítez Alemán, Héctor Eduardo. |
| La identificación precisa de heces de carnívoros silvestres es importante para describir sus hábitos alimenticios, sobre todo en aquellas especies elusivas o en riesgo. Sin embargo Identificar las heces de especies de carnívoros simpátricos no es una tarea sencilla. Por ello, las técnicas moleculares, son herramientas que brindan mayor precisión en la identificación de heces. El análisis de hábitos alimenticios aporta información valiosa para entender la estructura poblacional de los depredadores y sus presas, así como su importancia ecológica para generar estrategias de manejo y conservación de carnívoros y sus presas. Los objetivos de este estudio fueron identificar las heces de carnívoros extrayendo ADN mitocondrial de estas y amplificando un fragmento... |
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Palavras-chave: ADN mitocrondrial; Citocromo b; PCR-RFLP; Hábitos alimenticios; Carnívoros; Mitochondrial DNA; Cytochrome b; Food habits; Carnivores; Ganadería; Maestría. |
| Ano: 2014 |
URL: http://hdl.handle.net/10521/2291 |
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| Fu,Changzhen; Wang,Hong; Li,Yaokun; Wang,Jiali; Cheng,Gong; Wang,Hongbao; Yang,Wucai; Zan,Linsen. |
| Background CDIPT (CDP-diacylglycerol-inositol 3-phosphatidyltransferase, EC 2.7.8.11) was found on the cytoplasmic side of the Golgi apparatus and the endoplasmic reticulum. It was an integral membrane protein performing the last step in the de novo biosynthesis of phosphatidylinositol (PtdIns). In recent years, PtdIns has been considered to play an essential role in energy metabolism, fatty acid metabolic pathway and intracellular signal transduction in eukaryotic cells. Results In this study, the results of real-time polymerase chain reaction (PCR) showed that the expression of CDIPT gene was remarkably different in diverse tissues. We also detected the polymorphism of bovine CDIPT gene and analyzed its association with body measurement and meat quality... |
| Tipo: Journal article |
Palavras-chave: Body measurement traits; Gene expression; Meat quality traits; PCR-RFLP; SNP. |
| Ano: 2014 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000400004 |
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| Lee,Youngsub; Park,Sairom; Kim,Hun; Lee,Seung Kyu; Kim,Jin Woo; Lee,Hak Kyo; Jeong,Dong Kee; Lee,Sung Jin. |
| Background: The melanocortin-4 receptor gene (MC4R) plays an important role in regulating food intake and body weight in mammals. In the present study, we identified the MC4R gene in native Korean brown, brindle, and black cattle by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP), and investigated its association with economic traits. A total of 413 cattle from the three breeds were tested for backfat thickness, carcass weight, longissimus muscle area, and marbling score, and statistical data were analyzed using the SAS program. Results: The C allele had the highest frequency in brown and brindle cattle, and the G allele frequency was highest in black cattle. The C1069G SNP was significantly (p < 0.05) associated with only... |
| Tipo: Journal article |
Palavras-chave: Economic trait; Marbling scores; MC4R gene; PCR-RFLP; SNP. |
| Ano: 2013 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000500014 |
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| Zhan,Xiaoli; Jiang,Bijie; Ma,Xianghui; Fu,Changzhen; Zan,Linsen. |
| Background: We investigated the polymorphisms of the bovine chemokine receptor-like 1(CMKLR1) gene. The coding region of CMKLR1 was screened in Qinchuan cattle by PCR-RFLP technology. Results: In this study, we discovered two single nucleotide polymorphisms (SNPs) (264G > C and 762C > T) in the coding region of the CMKLR1 gene. Hence, we described the BmgT120l and Pdm1 PCR-RFLP methods for detecting the 64G > C and 762C > T mutations, respectively. PCR-RFLP and sequencing were used to analyze the two loci of CMKLR1 gene in 324 individuals, which were randomly selected from breeding populations. Furthermore, meat quality traits in another 80 Qinchuan individuals were analyzed by the comparison between the genotypes and their phenotypic data.... |
| Tipo: Journal article |
Palavras-chave: CMKLR1 gene; Meat quality traits; PCR-RFLP; Qinchuan cattle; SNP. |
| Ano: 2013 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000100003 |
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| Torres-Vázquez,José Antonio; Vázquez Flores,Felícitas; Montaldo,Hugo H; Ulloa-Arvizu,Raúl; Valencia Posadas,Mauricio; Gayosso Vázquez,Amanda; Alonso Morales,Rogelio Alejandro. |
| With the objective of estimating allele frequencies, and testing for population divergence for the CSN1S1 locus, genotypes of animals from five goat populations; Saanen (n = 97), Alpine (n = 81) Toggenburg (n = 92), local goats with external appearance similar to the Murciana-Granadina breed from Central Mexico (n = 26) and heterogeneous local animals denominated Mosaico Lagunero (n = 30), from Northern Mexico, were identified using PCR and Xmn1 PCR-RFLP methodology. For Saanen, Alpine and Toggenburg, the sum of E and F alleles had the largest frequencies (from 0.468 to 0.789), while for the groups local Murciana-Granadina and Mosaico Lagunero the sum of the most frequent allelic groups (A* and B*), were 0.385 and 0.533 respectively. Both local... |
| Tipo: Journal article |
Palavras-chave: CSN1S1; Gene frequencies; Genetic diversity; Goat milk; PCR-RFLP. |
| Ano: 2008 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000300007 |
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| Registros recuperados: 67 | |
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