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| Registros recuperados: 32 | |
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| Sun,Dan Dan; Ma,Xiao Xue; Hu,Jian; Tian,Yuan; Pang,Long; Shang,Hong; Cui,Long Zhu. |
| In order to obtain adequate information for the treatment of methicillin resistant Staphylococcus aureus (MRSA) infections, it is crucial to identify trends in epidemiological and antimicrobial resistance patterns of local S. aureus strains. Community and hospital acquired S. aureus isolates (n = 202) were characterized using staphylococcal cassette chromosome mec (SCCmec) typing, pulse field gel electrophoresis (PFGE) analysis, spa typing and minimal inhibitory concentration (MIC) determination. The prevalence of the Panton-Valentine leukocidine (pvl) and several antibiotic resistance genes among the isolates were also detected by PCR. All of the S. aureus isolates were susceptible to vancomycin, daptomycin and linezolid. Three hospital isolates were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: CA-and HA-MRSA; SCCmec; PFGE; Spa; Pvl gene. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702013000600010 |
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| Adesida,Solayide A.; Abioye,Olusegun A.; Bamiro,Babajide S.; Brai,Bartholomew I.C.; Smith,Stella I.; Amisu,Kehinde O.; Ehichioya,Deborah U.; Ogunsola,Folasade T.; Coker,Akitoye O.. |
| Staphylococcus aureus infections are growing problems worldwide with important implications in hospitals. The organism is normally present in the nasal vestibule of about 35% of apparently healthy individuals and its carriage varies between different ethnic and age groups. Staphylococcal nasal carriage among health workers is particularly important to establish new clones and track origin of infections during outbreak situations. To determine the carriage rate and compare the pulsed field gel patterns of the strains, nasal swabs were collected from 185 medical students in a teaching hospital in Lagos, Nigeria. Isolates of S. aureus were tested for heamolysin production, methicillin sensitivity and Pulsed Field Gel Electrophoresis (PFGE) was performed. The... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Staphylococcus aureus; PFGE; Nasal isolates; Carriage rate. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000100016 |
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| Pereira,Andrea S.; Andrade,Soraya S.; Monteiro,Jussimara; Sader,Helio S.; Pignatari,Antonio C.C.; Gales,Ana C.. |
| Increasing quinolone resistance has been reported worldwide, mainly among clinical isolates of Escherichia coli. The objectives of this study were to determine the susceptibility profile, the genetic relatedness, and the prevalence of the qnr gene among ciprofloxacin-resistant Escherichia coli isolated from distinct Brazilian hospitals. A total of 144 ciprofloxacin-resistant Escherichia coli were isolated from 17 Brazilian hospitals between January/2002 and June/2003. The antimicrobial susceptibility testing was performed by microdilution according to NCCLS. The presence of the qnr gene was initially screened by colony blotting, and then confirmed by PCR followed by DNA sequencing. Ninety-five urinary ciprofloxacin-resistant Escherichia coli were further... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Quinolone resistance; Escherichia coli; PFGE; Qnr. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000100011 |
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| Seki,Liliane Miyuki; Pereira,Polyana Silva; Conceição,Magda de Souza; Souza,Maria José; Marques,Elizabeth Andrade; Carballido,Jupira Miron; Carvalho,Maria Elisabeth Serqueira de; Assef,Ana Paula D'Alincourt Carvalho; Asensi,Marise Dutra. |
| OBJECTIVE: The present studywas designed to evaluate the molecular epidemiology of CTX-M producing Klebsiella pneumoniae, Enterobacter cloacae and Escherichia coli isolated from bloodstream infections at tertiary care hospitals in the State of Rio de Janeiro, Brazil. MATERIAL AND METHODS: A total of 231 nonduplicate Enterobacteriaceae were isolated from five Brazilian hospitals between September 2007 and September 2008. The antimicrobial susceptibility testing was performed by disk diffusion method according to the Clinical Laboratory Standard Institute. Isolates showing resistance to third-generation cephalosporins were screened for ESBL activity by the double-disk synergy test. The presence of blaCTX-M , blaCTX-M-15 and blaKPC genes was determined by... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bloodstream infections; Enterobacteriaceae; CTX-M-15; PFGE; KPC; Brazil. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702013000600003 |
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| Gales,Ana C.; Torres,Patrícia L.; Vilarinho,Dominique S. O.; Melo,Renata S.; Silva,Claudinéia F. L.; Cereda,Rosângela F.. |
| The genetic similarity of carbapenem-resistant Pseudomonas aeruginosa strains isolated in the Hospital Universitário São Francisco, Bragança Paulista, São Paulo, Brasil, was evaluated by pulsed field gel electrophoresis (PFGE). A unique clone was detected among 5 of 7 isolates, suggesting that cross-contamination might have played a role in the spread of carbapenem-resistant P. aeruginosa strains. Interestingly, a similar PFGE pattern was encountered in a P. aeruginosa strain isolated from Hospital São Paulo that was used as a PFGE control. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pseudomonas aeruginosa; PFGE; Carbapenem. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702004000400001 |
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| Almeida,Rosana Macedo de; Barbosa,André Victor; Lisbôa,Rodrigo de Castro; Santos,André Felipe das Mercês; Hofer,Ernesto; Vallim,Deyse Christina; Hofer,Cristina Barroso. |
| ABSTRACT The herein presented assay provided a bacteriological and molecular characterization of 100 samples of L. monocytogenes isolated from human (43) and food (57) sources, from several regions of Brazil, and collected between 1975 and 2013. Antigenic characterization defined 49% of serotype 4b samples, followed by 28% of serotype 1/2b, 14% of serotype 1/2c, 8% of serotype 1/2a, and 1% of serotype 3b. Both type of samples from human and food origin express the same serotype distribution. Multiplex PCR analysis showed 13 strains of type 4b with the amplification profile 4b-VI (Variant I). Virulence genes hly, inlA, inlB, inlC, inlJ, actA, plcA, and prfA were detected in all samples, highlighting a deletion of 105pb on the actA gene in 23% of serotype 4b... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Listeria monocytogenes; Listeriosis; Virulence; Food; PFGE; Brazil. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000300282 |
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| Lilenbaum,Walter; Kremer,Frederico; Ristow,Paula; Dellagostin,Odir; Bourhy,Pascale; Hartskeerl,Rudy; Vasconcellos,Silvio. |
| Two Leptospira sp. isolates were obtained by the first time from goats in Brazil and characterized by sequencing rrs, rpoB and secY genes, PFGE and typing with monoclonal antibodies. Both isolates are identical and belong to Leptospira santarosai. Analysis of the rrs and the rpoB genes sequences revealed 100% identity between the goat isolates and the Bananal reference strain. When secY sequences of the two isolates were compared to each other, it was observed that they had identical sequences. However, when compared to that of the Bananal reference strain, there were 15 mismatches along the 549 bp secY sequence. In conclusion, molecular methods are increasingly useful for the characterization of leptospires and allowed to identify those isolates of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Leptospira; Serogrouping; Sequencing; PFGE; Rrs. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400050 |
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| Bender,Eduardo André; Freitas,Ana Lúcia Peixoto de; Reiter,Keli Cristine; Lutz,Larissa; Barth,Afonso Luís. |
| In the past two decades the members of the genus Enterococcus have emerged as important nosocomial pathogens worldwide. In the present study, we evaluated the antimicrobial resistance and genotypic characteristics of 203 Enterococcus spp. recovered from different clinical sources from two hospitals in Porto Alegre, Rio Grande do Sul, Brazil. The species were identified by conventional biochemical tests and by an automated system. The genetic diversity of E. faecalis presenting high-level aminoglycoside resistance (HLAR) was assessed by pulsed-field gel electrophoresis of chromosomal DNA after SmaI digestion. The E. faecalis was the most frequent specie (93.6%), followed by E. faecium (4.4%). The antimicrobial resistance profile was: 2.5% to ampicillin,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Enterococcus; Resistance; HLAR; PFGE. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000300035 |
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| Lü,Hui; Yuan,Yuqi; Sun,Na; Bi,Zhenwang; Guan,Bing; Shao,Kun; Wang,Tongzhan; Bi,Zhenqiang. |
| Abstract Cholera continues to be a serious public health issue in developing countries. We analyzed the epidemiological data of cholera from 1976 to 2013 in Shandong Province, an eastern coastal area of China. A total of 250 Vibrio cholerae isolates were selected for PCR analysis of virulence genes and pulsed-field gel electrophoresis (PFGE). The analysis of the virulence genes showed that the positive rates for tcpA and tcpI were the highest among strains from the southwest region, which had the highest incidence rate of cholera. Low positive rates for tcpA, tcpI and ctxAB among isolates from after 2000 may be an influencing factor contributing to the contemporary decline in cholera incidence rates. Spatiotemporal serotype shifts (Ogawa, Inaba, Ogawa,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Vibrio cholerae; Epidemiology; PFGE; Virulence genes. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000100173 |
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| D'Azevedo,P.A.; Secchi,C.; Antunes,A.L.S.; Sales,T.; Silva,F.M.; Tranchesi,R.; Pignatari,A.C.C.. |
| In the last decades, coagulase-negative staphylococci (CoNS), especially Staphylococcus epidermidis have become an important cause of bloodstream infections. In addition, rates of methicillin-resistance among CoNS have increased substantially, leading to the use of glicopeptides for therapy. The objective of this study was to evaluate eleven consecutives clinically relevant cases of oxacillin-resistant CoNS bacteremia in a general hospital localized in São Paulo city, Brazil. Five different species were identified by different phenotypic methods, including S. epidermidis (5), S. haemolyticus (3), S. hominis (1), S. warneri (1) and S. cohnii subsp urealyticus (1). A variety of Pulsed Field Gel Electrophoresis profiles was observed by macrorestriction DNA... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Coagulase-negative staphylococci; Bacteremia; Oxacillin; PFGE. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000400006 |
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| Moemen,Dalia; Tawfeek,Doaa; Badawy,Wafaa. |
| Vancomycin resistant Enterococcus faecium (VREF) ia an emerging and challenging nosocomial pathogen. This study aimed to determine the prevalence, risk factors and clonal relationships between different VREF isolates in the intensive care units (ICUs) of the university hospitals in our geographic location. This prospective study was conducted from July, 2012 until September, 2013 on 781 patients who were admitted to the ICUs of the Mansoura University Hospitals (MUHs), and fulfilled the healthcare-associated infection (HAI) criteria. Susceptibility testing was determined using the disk diffusion method. The clonal relationships were evaluated with pulsed field gel electrophoresis (PFGE). Out of 52 E. faecium isolates, 12 (23.1%) were vancomycin resistant.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Enterococcus faecium; ICU; Vancomycin; Risk factors; PFGE. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000300777 |
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| Souza,Viviane; Nader Filho,Antonio; Melo,Poliana de Castro; Ferraudo,Guilherme Moraes; Ferraudo,Antônio Sérgio; Conde,Sandra de Oliveira; Fogaça Junior,Flavio Augusto. |
| The epidemiological relationships between isolated Staphylococcus aureus strains in milk samples of dairy cows, reagent to California Mastitis Test, individual and group milk was demonstrated in different sites of the production fluxogram, in 12 milk-producing farms in the Gameleira region, municipality of Sacramento MG Brazil, so that localization and transmission modes may be identified. Two hundred and forty-four strains out of 446 samples collected at several sites were isolated and bio-chemically characterized as coagulase-positive staphylococcus. Specific chromosome DNA fragment of the species Staphylococcus aureus was amplified to 106 strains and 103 underwent (PFGE). Samples' collection sites with the highest isolation frequency of Staphylococcus... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mastitis; Staphylococcus aureus; Bulk tank; PCR; PFGE. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400050 |
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| Angelini,Michelle; Stehling,Eliana Guedes; Moretti,Maria Luiza; Silveira,Wanderley Dias da. |
| Shigella spp., the human pathogen responsible for shigellosis, is highly infectious even at low levels. The incidence rate of shigellosis varies with geographical distribution, location human development index, and age groups, being higher among children aged under 5 years. In Brazil, a few works indicate that shigellosis cases are underestimated, with S. flexneri and S. sonnei strains being the major agents responsible for the shigellosis cases. The present study used pulsed field gel electrophoresis (PFGE) to investigate the molecular epidemiology of 119 strains of S. sonnei and S. flexneri isolated from shigellosis cases that occurred in the metropolitan areas of Ribeirão Preto and Campinas Cities, São Paulo Sate, southeast Brazil. The results indicated... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Shigella sonnei; Shigella flexneri; Epidemiology; Characterization; PFGE. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000300034 |
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| Almeida,Margarete Teresa Gottardo; Rubio,Fernando Gôngora; Garcia,Doroti Oliveira; Pavarino-Bertelli,Érika Cristina; Rossit,Andrea Regina Baptista; Bando,Silvia Yano; Silbert,Suzane; Goloni-Bertollo,Eny Maria; Soares,Márcia Maria Costa Nunes; Martinez,Marina Baquerizo. |
| Stenotrophomonas maltophilia is a Gram-negative bacillus, which is becoming widely recognized as an important nosocomial pathogen. The main objective of this study was to evaluate the genetic relatedness, by random amplified polymorphic DNA (RAPD) and pulsed-field gel electrophoresis (PFGE) of 86 clinical isolates of S. maltophilia (colonization 22, infection 64) obtained from 79 hospitalized patients, from different geographic regions of São Paulo State. The genotypic analysis performed by RAPD and PFGE was used in 24 isolates for genetic identity confirmation. The results were congruent between the two methods but it was not possible to link genetic profiles with the studied variables, clinical state and geographic area, probably due to the great... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Stenotrophomonas maltophilia; RAPD; PFGE. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000200017 |
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| Registros recuperados: 32 | |
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