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	Provedor de dados Genet. Mol. Biol. (2) Electron. J. Biotechnol. (1) Autor Asurmendi,Sebastián (1) Bazzini,Ariel A (1) Benedito,Vagner Augusto (1) Campos-Pereira,Tiago (1) Faria,Laura (1) Figueira,Antonio (1) Freitas-Astúa,Juliana (1) Hopp,H. Esteban (1) Maia,Ivan de Godoy (1) Mongelli,Vanesa C (1) Sassaki,Flávio Tetsuo (1) del Vas,Mariana (1) Mais... Palavra-chave Gene silencing (3) PTGS (3) CitEST (1) EST (1) Eucalyptus (1) GFP (1) HC-Pro (1) PDS (1) Protocols (1) RNA silencing (1) TRV (1) Teaching (1) Transgenic (1) Transient expression (1) VIGS (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Journal article (1) Ano 2007 (2) 2005 (1) País Brazil (2) Chile (1) Idioma Inglês (3)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 3 Primeira ... 1 ... Última A practical approach to the understanding and teaching of RNA silencing in plants Electron. J. Biotechnol. Bazzini,Ariel A; Mongelli,Vanesa C; Hopp,H. Esteban; del Vas,Mariana; Asurmendi,Sebastián. Gene silencing, also called RNA interference (RNAi) is a specific mechanism of RNA degradation involved in gene regulation, development and defense in eukaryotic organisms. It became an important subject in the teaching programs of molecular biology, genetics and biotechnology courses in the last years. The aim of this work is to provide simple and inexpensive assays to understand and teach gene silencing using plants as model systems. The use of transient and permanent transgenic plants for expressing reporter genes, like those derived from jellyfish green fluorescent protein (gfp) encoding gene, provides a nice, colorful and conclusive image of gene silencing. Three experimental approaches to evidence RNA silencing are depicted. In the first approach... Tipo: Journal article Palavras-chave: Gene silencing; GFP; HC-Pro; PDS; Protocols; PTGS; Teaching; Transgenic; Transient expression; TRV; VIGS. Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000200002 Genetic machinery for RNA silencing and defense against viruses in Citrus Genet. Mol. Biol. Benedito,Vagner Augusto; Faria,Laura; Freitas-Astúa,Juliana; Figueira,Antonio. RNA silencing mechanisms are conserved throughout eukaryotic evolution, possibly due to their importance in viral resistance and other aspects of cell biology. Here, we explored the Citrus EST (CitEST) database in search of sequences related to the most important known genes involved in RNA silencing. Transcripts strongly matching Argonaute (AGO), Dicer-like (DCL), Hua enhancer (HEN), and RNA-dependent RNA Polymerase (RdRP) were found in many of the citrus libraries. The reads were clustered and quantified. This shows that post-transcriptional gene silencing apparatus is active in citrus. It seems plausible that a better understanding of the players of RNA silencing in Citrus spp. and related genera may help create new tools to defeat the viral diseases... Tipo: Info:eu-repo/semantics/article Palavras-chave: CitEST; Gene silencing; PTGS. Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500027 The post-transcriptional gene silencing pathway in Eucalyptus Genet. Mol. Biol. Sassaki,Flávio Tetsuo; Campos-Pereira,Tiago; Maia,Ivan de Godoy. Post-transcriptional gene silencing (PTGS) is a conserved surveillance mechanism that identifies and cleaves double-stranded RNA molecules and their cellular cognate transcripts. The RNA silencing response is actually used as a powerful technique (named RNA interference) for potent and specific inhibition of gene expression in several organisms. To identify gene products in Eucalyptus sharing similarities with enzymes involved in the PTGS pathway, we queried the expressed sequence tag database of the Brazilian Eucalyptus Genome Sequence Project Consortium (FORESTs) with the amino acid sequences of known PTGS-related proteins. Among twenty-six prospected genes, our search detected fifteen assembled sequences encoding products presenting high level of... Tipo: Info:eu-repo/semantics/article Palavras-chave: Gene silencing; PTGS; RNA silencing; EST; Eucalyptus. Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000400003 Registros recuperados: 3 Primeira ... 1 ... Última

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