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| Gonçalves-de-Albuquerque,Suênia da Cunha; Silva,Rômulo Pessoa e; Morais,Rayana Carla Silva de; Trajano-Silva,Lays Adrianne Mendonça; Régis-da-Silva,Carlos Gustavo; Brandão Filho,Sinval Pinto; Paiva-Cavalcanti,Milena de. |
| Background : Molecular biological methods have become increasingly relevant to the diagnosis and control of infectious diseases, such as leishmaniasis. Since various factors may affect the sensitivity of PCR assays, including DNA yield and purity, an optimal extraction method is pivotal. Losses of a parasite’s DNA during extraction may significantly impair its detection by PCR and lead to false-negative results. This study proposes a triplex PCR assay targeting the parasite’s DNA, an external control (pUC18) and an internal control (G3PD) for accurate diagnosis of leishmaniasis.Results : Two primer pairs were designed to detect the plasmid pUC18 and a triplex PCR assay targeting theLeishmania braziliensiskinetoplast DNA, the external control and the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Extraction control; Multiplex PCR; PUC18; Leishmaniasis; Diagnosis; False-negative result. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200326 |
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