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| Registros recuperados: 82 | |
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| Rohfritsch, Audrey; Bierne, Nicolas; Boudry, Pierre; Heurtebise, Serge; Lapegue, Sylvie. |
| Originating from the north eastern Asia, Crassostrea gigas has been introduced and translocated, mainly for aquaculture purpose, into several European countries (from Norway to Portugal and in the Mediterranean Sea) (1). Although highly variable, the invasiveness pattern of C. gigas has been demonstrated in several countries and therefore considered as a pest or a noxious species in those areas (2 and 3).Our project aims at identifying the characteristics of such a flourishing species: can its success be explained by chance and/or global warming only or does it exhibit a more important potential of adaptation than other species? Therefore we developed a population genomics approach, known as "genome scan". It corresponds to the study of numerous loci... |
| Tipo: Text |
Palavras-chave: Invasive; AFLP; Genome scan; Crassotrea gigas; Pacific oyster. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/acte-4805.pdf |
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| Le Goic, Nelly; Hegaret, Helene; Boulais, Myrina; Beguel, Jean-philippe; Lambert, Christophe; Fabioux, Caroline; Soudant, Philippe. |
| The Pacific oyster Crassostrea gigas accounts for a large part of shellfish aquaculture production worldwide. Aspects of morphological and functional characteristics of oyster oocytes remain poorly documented, and traditional techniques, such as microscopic observations of shape or fertilization rate, are time and space consuming. The purpose of this study was to assess for the first time viability and reactive oxygen species (ROS) production of Pacific oyster oocytes using flow cytometry (FCM) and to apply this method to determine oocyte responses to in vitro exposure to the toxic dinoflagellate Alexandrium minutum. A culture of A. minutum caused a significant increase in oocyte ROS production, which gradually increased with the age of the culture, but... |
| Tipo: Text |
Palavras-chave: Pacific oyster; Gamete quality; ROS production; Viability; Flow cytometry; Ecotoxicology. |
| Ano: 2014 |
URL: https://archimer.ifremer.fr/doc/00216/32760/31321.pdf |
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| Lallias, Delphine; Boudry, Pierre; Batista, Frederico; Beaumont, Andy; King, Jonathan W.; Turner, John R.; Lapegue, Sylvie. |
| The Pacific oyster, Crassostrea gigas, native to northeast Asia, is one of the most important cultured shellfish species. In Europe, Pacific oysters first settled along the Atlantic coasts of France at the end of the 1960s but rapidly spread and are now widely established. Twenty-two sites in the United Kingdom (UK), Ireland, Denmark, France and Spain were sampled to assess genetic diversity and differentiation. Hatchery-propagated stocks from two hatcheries located in the UK also were included. Two main genetic clusters were identified from pairwise genetic differentiation indexes, Bayesian clustering methods or neighbour-joining analysis, based on 7 microsatellite loci: (1) a Northeast cluster (including feral samples from East England, Ireland and... |
| Tipo: Text |
Palavras-chave: Invasion genetics; Marine mollusc; Microsatellites; MtDNA; Pacific oyster; Crassostrea gigas. |
| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00268/37973/36052.pdf |
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| Gagnaire, Beatrice; Thomas Guyon, H; Burgeot, Thierry; Renault, Tristan. |
| The shellfish industry is an important economic activity in France, occurring mostly in estuarine zones subject to pollution due to anthropogenic activities. The harmful effects of pollutants on species inhabiting these estuarine zones are not well known. Among marine species, bivalve mollusks---particularly Pacific oyster, Crassostrea gigas-may serve a model of interest. The species is sedentary and filter-feeding, which favors bioaccumulation of pollutants in their tissues. Oysters may be suitable for studies on disturbance by pollutants of physiological activities, among which defense mechanisms are poorly documented in bivalves. In this study, effects of pollutants on hemocyte functions were monitored in Pacific oyster, C. gigas. Hemocytes were exposed... |
| Tipo: Text |
Palavras-chave: Toxicity; Pesticides; PCBs; PAHs; Crassostrea gigas; Pacific oyster; Hemocytes; Flow cytometry; Cellular activities. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-1695.pdf |
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| Gagnaire, Beatrice; Gay, Melanie; Huvet, Arnaud; Daniel, Jean-yves; Saulnier, Denis; Renault, Tristan. |
| To assess the impact of pollution induced by pesticides on Pacific oyster, Crassostrea gigas, health in France, in vivo effects of combined pesticide exposure and bacterial challenge on cell activities and gene expression in hemocytes were tested using flow cytometry and real-time PCR. As a first step, an in vivo model of experimental contamination was developed. Pacific oysters were exposed to a mixture of eight pesticides (atrazine, glyphosate, alachlor, metolachlor, fosetyl-alumimium, terbuthylazine, diuron and carbaryl) at environmentally relevant concentrations over a 7-day period. Hemocyte parameters (cell mortality, enzyme activities and phagocytosis) were monitored using flow cytometry and gene expression was evaluated by real-time PCR (RT-PCR).... |
| Tipo: Text |
Palavras-chave: Gene expression; Flow cytometry; Vibrio splendidus; Pathogenesis; Phagocytosis; Hemocyte; Pesticides; Bivalve immunity; Crassostrea gigas; Pacific oyster. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-3022.pdf |
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| Ernande, Bruno; Boudry, Pierre; Heurtebise, Serge; Haure, Joel; Martin, Jean-louis. |
| Introduction : Growth and survival are the life-history traits of the most interest for oyster production since they determine biomass output (growth x survival). This study aimed to investigate whether there is genetic basis for growth, survival and their plasticity to know if a selection program is conceivable for these traits. Material and Methods : Nested half-sibs mating design was used to produce families of oysters: 5 males were each mated to three females producing 5 half-sibs families, each containing 3 full-sib families. At 15 months of age (May 1999), each full-sibs family was randomly split into five groups which were given different treatments. Two groups were placed in a constant environment: one with low food resources and another with high... |
| Tipo: Text |
Palavras-chave: Genotyic; Plasticity; Survival; Growth; Genetic; Crassostrea gigas; Pacific oyster. |
| Ano: 2000 |
URL: http://archimer.ifremer.fr/doc/2000/acte-3498.pdf |
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| Le Goic, Nelly; Hegaret, Helene; Fabioux, Caroline; Miner, Philippe; Suquet, Marc; Lambert, Christophe; Soudant, Philippe. |
| The toxic dinoflagellate Alexandrium catenella recurrently blooms on the coasts of France and produces Paralytic Shellfish Toxins (PSTs) that accumulate in bivalves. These toxins can affect various physiological functions including reproduction. The present study aims to validate measurements of sperm viability, DNA content and mitochondrial membrane potential in Pacific oyster Crassostrea gigas using flow cytometry coupled with fluorescent markers, and to use these measurements to assess the cellular parameters of sperm from Pacific oysters exposed to A. catenella. These parameters may influence fertilization, embryogenesis and larval development in free-spawning shellfish. Sperm viability and DNA content estimation were assessed using SYBR-14, which only... |
| Tipo: Text |
Palavras-chave: Harmful algal bloom; Sperm quality; Cellular parameters; Flow cytometry; Pacific oyster; Crassostrea gigas; Alexandrium catenella. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00157/26853/24982.pdf |
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| Samain, Jean-francois; Degremont, Lionel; Soletchnik, Patrick; Ropert, Michel; Bedier, Edouard; Mazurie, Joseph; Mazurie, Joseph; Martin, Jean-louis; Moal, Jeanne; Mathieu, Michel; Pouvreau, Stephane; Lambert, Christophe; Escoubas, Jean-michel; Nicolas, Jean-louis; Le Roux, Frédérique; Renault, Tristan; Burgeot, Thierry; Bacher, Cedric; Boudry, Pierre. |
| Complex interactions between oyster, environment and pathogens were observed during the MOREST (2001-2005) project on summer mortality events of Crassostrea gigas oysters in France. The talk will show how nutrition level increasing reproductive intensity generates a risk for pathogen infection under synergistic effect of temperature and stress. Moreover, a genetic component is associated to specific reproductive performances and spawning behaviour. To better understand origin of such resistance or susceptibility to summer mortality, other characteristics as stress susceptibility, energy management and hemocyte activities of oysters from the two phenotypes were compared. This first comparison suggests interactions between stress, reproduction and defence... |
| Tipo: Text |
Palavras-chave: Genetic; Pathologie; Mortality; Crassostrea gigas; Pacific oyster; Reproduction; Nutrition; Physilogy. |
| Ano: 2006 |
URL: https://archimer.ifremer.fr/doc/2006/acte-3467.pdf |
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| Petton, Bruno; Le Grand, Jacqueline; Riobe, Marion; Mingant, Christian; Ratiskol, Dominique; Petton, Sebastien; Alunno-bruscia, Marianne. |
| Depuis 2008, des mortalités massives d’huîtres creuses âgées de moins d’un an sont relevées sur le littoral français dès que la température de l’eau de mer atteint le seuil de 16°C. Ces mortalités de naissains sont associées à la détection du virus OsHV-1 μVar. Depuis 2010, des travaux de qualification zoo-sanitaire ont montré qu’environ un lot de naissains de captage sur deux était infecté par le virus avant l’apparition des mortalités dans le milieu naturel. En 2014, dans le cadre de la première action QUALIF, les résultats de la qualification zoo-sanitaire des naissains du captage de l’année 2013 à l’échelle nationale ont mis en évidence le caractère infectieux des maladies développées par ces naissains porteurs latents d’OsHV-1 μVar. L’objectif 2015 du... |
| Tipo: Text |
Palavras-chave: Huitre creuse; Naissain; Maladie; OsHV-1 μVar; Epreuve thermique.; Pacific oyster; Seed; Disease; OsHV-1 μVar; Thermal challenge.. |
| Ano: 2016 |
URL: http://archimer.ifremer.fr/doc/00335/44587/44305.pdf |
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| Gagnaire, Beatrice; Soletchnik, Patrick; Faury, Nicole; Kerdudou, Nolwenn; Le Moine, Olivier; Renault, Tristan. |
| Internal defence mechanisms of bivalves include the hemocytes which assume several functions. Relationships exist between environmental factors, physiological state of oysters, defence system and pathogens. Hatchery oysters are more and more produced and reared in the field. The characteristics and particularly the defence system of these animals are poorly documented. In this context, two field trials were carried out from May to September 2002 and from April to July 2003 in order to monitor hemocyte parameters of diploid oysters from natural beds and hatchery-produced diploid oysters using flow cytometry. Several hemocyte parameters (cell mortality, granulocyte percentage, phagocytosis, esterase and peroxidase activities) were monitored. For both oyster... |
| Tipo: Text |
Palavras-chave: Hatchery; Natural beds; Diploids; Flow cytometry; Peroxidase activity; Esterase activity; Phagocytosis; Hemocytes; Crassostrea gigas; Pacific oyster. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-3119.pdf |
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| Barbosa-solomieu, Valerie; Renault, Tristan; Travers, Marie-agnes. |
| Massive mortality outbreaks in cultured bivalves have been reported worldwide and they have been associated with infection by a range of viral and bacterial pathogens. Due to their economic and social impact, these episodes constitute a particularly sensitive issue in Pacific oyster (Crassostrea gigas) production. Since 2008, mortality outbreaks affecting C. gigas have increased in terms of intensity and geographic distribution. Epidemiologic surveys have lead to the incrimination of pathogens, specifically OsHV-1 and bacteria of the Vibrio genus, in particular Vibrio aestuarianus. Pathogen diversity may partially account for the variability in the outcome of infections. Host factors (age, reproductive status…) including their genetic background that has... |
| Tipo: Text |
Palavras-chave: Pacific oyster; Spat; OsHV-1; Vibrio aestuarianus; Mortality. |
| Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00274/38534/37051.pdf |
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| Pouvreau, Stephane. |
| La conchyliculture, et principalement l’élevage de l’huître creuse, Crassostrea gigas, constitue la principale activité aquacole française. Cette activité repose, en grande partie, sur le recrutement naturel de l’espèce qui assure 60 à 70% des besoins en jeunes huîtres (naissain) : cette activité de collecte s’appelle le captage. Les deux principaux centres de captage en France sont les bassins d’Arcachon et de Marennes-Oléron. Or, depuis une dizaine d'années, sur le bassin d'Arcachon, le captage devient très variable: à des années de captage nul (par exemple 2002, 2005, 2007) ou faible (2009, 2010, 2011) succèdent des années excellentes voire pléthoriques (2003, 2006, 2008, 2012, 2014). A Marennes-Oléron, cette variabilité existe, mais s’avère beaucoup... |
| Tipo: Text |
Palavras-chave: Huître creuse; Naissain; Recrutement; Captage; Larves; Cycle de reproduction; Changement climatique; Pacific oyster; Spat; Recruitment; Seed collecting; Larvae; Reproductive cycle; Climate change. |
| Ano: 2016 |
URL: http://archimer.ifremer.fr/doc/00334/44533/44240.pdf |
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| Renault, Tristan; Stokes, Nancy; Chollet, Bruno; Cochennec, Nathalie; Berthe, Franck; Gerard, Andre; Burreson, Eugene. |
| Two cases of haplosporidian infection occurred during 1993 in Pacific oysters Crassostrea gigas from the French Atlantic coast. The localization and ultrastructure of the plasmodia are described. In situ hybridization of infected tissue sections was conducted with DNA probes for oyster-infecting haplosporidians. The Haplosporidium nelsoni-specific DNA probe MSX1347 hybridized with the C. gigas parasite, and the H, costale-specific probe SSO1318 did not hybridize. Total genomic DNA was extracted from the infected tissue sections for polymerase chain reaction (PCR) amplification of the haplosporidian. PCR amplifications with H. nelsoni-specific primers and with 'universal' actin primers did not yield the expected products of 573 and 700 bp, respectively. A... |
| Tipo: Text |
Palavras-chave: Haplosporidium nelsoni; Haplosporidiosis; Crassostrea gigas; Pacific oyster. |
| Ano: 2000 |
URL: http://archimer.ifremer.fr/doc/2000/publication-821.pdf |
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| Registros recuperados: 82 | |
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