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Limited vegetative compatibility as a cause of somatic recombination in Trichoderma pseudokoningii BJM
Barcellos,Fernando Gomes; Hungria,Mariangela; Pizzirani-Kleiner,Aline Aparecida.
With the aim of a better characterization of the somatic recombination process in Trichoderma pseudokoningii, a progeny from crossings between T. pseudokoningii strains contrasting for auxotroph markers was characterized by RAPD markers and PFGE (electrophoretic karyotype). Cytological studies of the conidia, conidiogenesis and heterokaryotic colonies were also performed. The genotypes of the majority of the recombinant strains analyzed were similar to only one of the parental strains and the low frequency of polymorphic RAPD bands suggested that the nuclear fusions may not occur into the heterokaryon. In some heterokaryotic regions the existence of intensely staining hyphae might be related to cell death. We proposed that a mechanism of somatic...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Somatic recombination; Limited vegetative compatibility; Trichoderma pseudokoningii; Parasexuality.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400050
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