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	Provedor de dados Anais da ABC (AABC) (1) Autor ALMEIDA,SINARA M. (1) BELTRÃO,EDUARDO I.C. (1) BEZERRA,SÉRGIO A. (1) CARVALHO JÚNIOR,LUIZ B. (1) RÊGO,MOACYR J.B.M. (1) Palavra-chave Concanavalin A (1) Immobilization (1) Magnetization (1) Parkia pendula seed gum (1) Tipo do documento Info:eu-repo/semantics/article (1) Ano 2014 (1) País Brazil (1) Idioma Inglês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 1 Primeira ... 1 ... Última Magnetic Parkia pendula seed gum as matrix for Concanavalin A lectin immobilization and its application in affinity purification Anais da ABC (AABC) RÊGO,MOACYR J.B.M.; ALMEIDA,SINARA M.; BEZERRA,SÉRGIO A.; CARVALHO JÚNIOR,LUIZ B.; BELTRÃO,EDUARDO I.C.. The present work aimed to magnetize Parkia pendula seeds gum and use it as a matrix for Concanavalin A covalent immobilization. This composite was applied in affinity purification of glycoconjugates. Parkia pendula seeds were hydrated and the gum provenient from the supernatant was precipitated and washed with ethanol and dried. The gum was magnetized in co-precipitation using solutions of Fe+2 and Fe+3. Matrix activation was accomplished with NaIO4. Magnetized Parkia pendula seeds gum with covalently immobilized Concanavalin A was used as an affinity matrix for the recognition of bovine serum fetuin glycoprotein. Fetuin elution was carried out with a solution of glucose (300mM) and evaluated through SDS-PAGE. The efficiency of lectin immobilization and... Tipo: Info:eu-repo/semantics/article Palavras-chave: Immobilization; Magnetization; Parkia pendula seed gum; Concanavalin A. Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652014000301351 Registros recuperados: 1 Primeira ... 1 ... Última

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