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| Dias,F.S.; Santos,I.F.; Franco,R.M.; Nascimento,E.R.. |
| Pathogenic microorganisms can reside transiently or permanently in the gallbladder of cattle. Thus, during slaughter, more attention should be given to the gastrointestinal tract, especially to the accessory organ, the gallbladder. The main aim of this study was to characterize the bacterial microbiota present in bile and gallbladder epithelium of cattle slaughtered in a slaughtering plant under sanitary conditions and to evaluate the antimicrobial resistance in strains of the genus Staphylococcus. Thirty intact gallbladders were collected and the in bile and epithelium were researched for the presence of Aerobic Mesophilic Heterotrophic Bacteria (AMHB), Staphylococcusspp., total Enterobacteriaceae, Enterococcus spp. and Salmonella spp. The frequency of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cattle gallbladder; Pathogenic microorganisms; Persistent carrier; Public health. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000300641 |
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| MENDONÇA,Juliana França Monteiro de; VIEIRA,Felipe de Oliveira; FONSECA,Isabela; RIBEIRO,João Batista; ARCURI,Edna Froeder; BORGES,Maria de Fátima; BORGES,Cristiano Amâncio Vieira; SÁ,Jaqueline Flaviana Oliveira de; MARTINS,Marta Fonseca. |
| Abstract Real-time PCR (qPCR) has been used for rapid identification of Salmonella Typhimurium and Staphylococcus aureus in dairy foods, but is unable to differentiate viable and unviable pathogens. Ethidium bromide monoazide (EMA), a DNA-intercalating agent, can detect only viable cells because selectively enter cells considered unviable and bind to their DNA, inhibiting its amplification during qPCR. The objective was to establish a protocol for detection of viable Salmonella Typhimurium and S. aureus, experimentally inoculated in coalho cheese, by the use of EMA combined with qPCR. The protocol was effective for the identification of viable Salmonella Typhimurium in coalho cheese but not for the S. aureus cells. Concentrations of viable Salmonella... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cell viability; Dairy products; DNA-intercalating agents; Foodborne diseases; Pathogenic microorganisms. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612019000600690 |
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| SCHUH,Vanessa; SCHUH,Janaína; FRONZA,Nei; FORALOSSO,Fabiana Bortolini; VERRUCK,Silvani; VARGAS JUNIOR,Alvaro; SILVEIRA,Sheila Mello da. |
| Abstract The aim of this study was to evaluate the hygienic-sanitary quality of minimally processed vegetables commercialized in the city of Concórdia, Santa Catarina State, Brazil. Eight distinct products, sold in 3 different supermarkets, totalizing 24 samples were evaluated. Of the analyzed samples, just one was in discordance with the microbiological parameters established by the Brazilian legislation (210 MPN/g of coliforms at 45 °C for Kale 2). Beyond the absence of Salmonella sp. indicated in legislation, all samples also indicated the absence of positive coagulase staphylococci and Listeria monocytogenes. The most expressive microbial counts were obtained when evaluating the aerobic mesophilic microorganisms (ranging from 2.8×102 to 2.7×107), and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Minimally processed vegetables; Hygiene; Pathogenic microorganisms; Food quality. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612020000200290 |
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| Silveira,Débora Rodrigues da; Lopes,Nathalie Almeida; Gonzalez,Helenice de Lima; Timm,Cláudio Dias. |
| Dulce de leche is a food obtained by concentration and heating of milk with the addition of sucrose. The common practice of opening the dulce de leche containers in retail markets can lead to food contamination by Salmonella. The objective of this study was to evaluate the survivability of Salmonellaenterica subsp. enterica serotypes Typhimurium, Enteritidis, Infantis and Derby in pasty dulce de leche. Aliquots of this sweet were experimentally contaminated with these microorganisms and later analyzed to evaluate microorganism viability after storage for 0, 1, 2, 3, 5, 10, and 20 days. Salmonella was recovered up to the 20th day. These results are a warning about the need to adopt proper sanitary-hygienic measures for handling and packaging this food... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Food safety; Foodborne diseases; Pathogenic microorganisms. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612012000400012 |
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| Branco,Paula Maria Pilotto; Fernandes,Aline; Cangani,Max Ternero; Souza-Pollo,Andressa de; Lucas Júnior,Jorge de; Amaral,Luiz Augusto do. |
| ABSTRACT: The objective of this study was to evaluate the effects of the addition of sugarcane juice on the population dynamics of Escherichia coli and the presence of Shiga-toxigenic E. coli (STEC) during the anaerobic codigestion of dairy cattle manure. For the overall analyses at the end of a hydraulic retention time of 90 days, ten two-liter batch-type biodigesters were divided into two treatment groups: biodigester containing manure and water (MW) and the biodigester containing manure, water and sugarcane juice (MSC). For monitoring the population dynamics and presence of microorganisms, pH, and volatile acidity, tests were carried out every ten days, on 36 smaller-scale batch biodigesters made of one-liter plastic bottles (18 for each treatment). The... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Biodigestion anaerobic; Environmental sustainability; Pathogenic microorganisms; Manure treatment. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000300452 |
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