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	Provedor de dados Ciênc. Tecnol. Aliment. (2) Acta Botanica (1) Autor AMAYA-LLANO,Silvia Lorena (1) DÍAZ-CRUZ,Claudio Alonso (1) GONZÁLEZ-JASSO,Eva (1) Gonzalez,Samantha Lemke (1) MORALES-SÁNCHEZ,Eduardo (1) REGALADO-GONZÁLEZ,Carlos (1) Rosso,Neiva Deliberali (1) Surgun-Acar,Yonca (1) VELAZQUEZ,Gonzalo (1) İşkil,Rabia (1) Mais... Palavra-chave Pectin methylesterase (3) 24-Epibrassinolide (1) Arabidopsis thaliana (1) Ataulfo mango (1) Boron (1) Cellulose synthase (1) Expansin (1) Gene expression (1) Pectin (1) Potentiometric procedure (1) Residual activity (1) Thermal inactivation (1) Xyloglucan endotransglucosylase/hydrolase (1) Mais... Tipo do documento Info:eu-repo/semantics/article (3) Ano 2018 (1) 2016 (1) 2011 (1) País Brazil (3) Idioma Inglês (3)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 3 Primeira ... 1 ... Última Expression analysis of cell wall assembly and remodelling-related genes in Arabidopsis roots subjected to boron stress and brassinosteroid at different developmental stages Acta Botanica İşkil,Rabia; Surgun-Acar,Yonca. ABSTRACT Plant cell walls are affected by many biotic and abiotic stress conditions. The aim of this study is to determine the effects of 24-Epibrassinolide (EBL) on some cell wall-related genes in root tissue of five- and ten-week-old Arabidopsis thaliana plants exposed to boron (B) deficiency (0 µM) or toxicity (3000 µM) at the transcriptional level. Expressions of the genes that encode cellulose synthase (CESA1, CESA4, CESA6 and CESA8), cellulose synthase-like (CSLB5), expansin (EXPA5, EXPA8 and EXPA14) and cell wall protein (SEB1) decreased under conditions of B deficiency and toxicity. EBL treatments, in general, led the expressions of these genes to reduce significantly. Expressions of xyloglucan endotransglucosylase/hydrolase genes (XTH21 and XTH23)... Tipo: Info:eu-repo/semantics/article Palavras-chave: 24-Epibrassinolide; Arabidopsis thaliana; Boron; Cellulose synthase; Gene expression; Expansin; Pectin methylesterase; Xyloglucan endotransglucosylase/hydrolase. Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062018000400546 Determination of pectin methylesterase activity in commercial pectinases and study of the inactivation kinetics through two potentiometric procedures Ciênc. Tecnol. Aliment. Gonzalez,Samantha Lemke; Rosso,Neiva Deliberali. Pectinases are enzymes that degrade pectic substances and are widely used in juice and fruit beverages to improve the quality of the process. The objective of this study was to determine the optimum pH and temperature of two samples of commercial pectinases and propose an alternative procedure to determine the residual activity comparing the data with those of the traditional procedure. The pectin methylesterase (PME) activity in Pectinex 100 L Plus and Panzyn Clears was determined by potentiometry. The reaction consisted of 5.00 mg.mL-1 apple pectin, 0.100 mol.L-1 NaCl, and 50 µL enzyme to a total volume of 30 mL. The pectin reaction in the presence of PME in all experiments revealed a first order kinetics. The PME in the two enzyme preparations showed... Tipo: Info:eu-repo/semantics/article Palavras-chave: Pectin; Pectin methylesterase; Potentiometric procedure; Residual activity. Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612011000200020 Thermal inactivation kinetics of partially purified mango pectin methylesterase Ciênc. Tecnol. Aliment. DÍAZ-CRUZ,Claudio Alonso; REGALADO-GONZÁLEZ,Carlos; MORALES-SÁNCHEZ,Eduardo; VELAZQUEZ,Gonzalo; GONZÁLEZ-JASSO,Eva; AMAYA-LLANO,Silvia Lorena. Abstract Kinetic parameters of thermal inactivation of pectin methylesterase (PME) in a partially purified mango enzyme extract were determined. The PME of mango partially purified by salting out showed different patterns of thermal inactivation, indicating the presence of a thermostable fraction at 70 °C and a thermolabile fraction at lower temperatures. The inactivation of the thermostable fraction exhibited a linear behavior that yielded a z-value of 9.44 °C and an activation energy (Ea) of 245.6 kJ mol-1 K-1 using the Arrhenius model. The thermostable mango PME fraction represented 17% of total crude enzyme extract, which emphasizes the importance of residual enzyme activity after heat treatment. Tipo: Info:eu-repo/semantics/article Palavras-chave: Thermal inactivation; Pectin methylesterase; Ataulfo mango. Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612016000200282 Registros recuperados: 3 Primeira ... 1 ... Última

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