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Downstream processing of pectinase produced by Aspergillus niger in solid state cultivation and its application to fruit juices clarification 116
Poletto,Patrícia; Borsói,Caroline; Zeni,Mara; Silveira,Mauricio Moura da.
In this work, a protocol for the formulation of an enzyme concentrated product to be applied in fruit juice treatment is described. Downstream processing conditions for the recovery and concentration of pectinases produced by the new strain Aspergillus niger LB-02-SF in solid state cultivation were assessed. The solid-liquid ratio in the extraction step of pectinases recovery from the cultivated media was evaluated and the highest activity was obtained with a solid-liquid ratio of 1:10. The crude extract was concentrated by ultrafiltration and the total pectinase (TP) activity was 73.6-fold concentrated in relation to the crude extract, and a final TP titer of 663 U mL–1 was obtained with 73.7% of recovery yield. KCl and different glycerol concentrations...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Solid-state cultivation; Downstream processing; Pectinase stability; Pectinase formulation; Juice clarification.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612015000200391
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